EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into transcriptional regulation by the helicase RECQL5.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 9, Page 1721-1730, Year 2025
掲載日	2025年7月7日
著者	Alfredo Jose Florez Ariza / Nicholas Z Lue / Patricia Grob / Benjamin Kaeser / Jie Fang / Susanne A Kassube / Eva Nogales /
PubMed 要旨	Transcription poses a major challenge for genome stability. The RECQL5 helicase helps safeguard genome integrity and is the only member of the human RecQ helicase family that directly binds to RNA ...Transcription poses a major challenge for genome stability. The RECQL5 helicase helps safeguard genome integrity and is the only member of the human RecQ helicase family that directly binds to RNA polymerase II (Pol II) and affects its progression. While RECQL5 mitigates transcription stress in cells, the molecular mechanism underlying this phenomenon is unclear. Here, we use cryo-electron microscopy to determine the structures of stalled human Pol II elongation complexes (ECs) bound to RECQL5. Our structures reveal the molecular interactions stabilizing RECQL5 binding to the Pol II EC and highlight its role as a transcriptional roadblock. Additionally, we find that, in its nucleotide-free state, RECQL5 twists the downstream DNA in the EC and, upon nucleotide binding, undergoes a conformational change that allosterically induces Pol II toward a post-translocation state. We propose that this mechanism may help restart Pol II elongation and, therefore, contribute to reducing transcription stress.
リンク	Nat Struct Mol Biol / PubMed:40624164 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.7 Å
構造データ	48071 9ehz EMDB-48071, PDB-9ehz: Cryo-EM structure of Human RNA polymerase II Elongation Complex in an Intermediate Translocation State 手法: EM (単粒子) / 解像度: 2.6 Å 48073 9ei1 EMDB-48073, PDB-9ei1: Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the absence of nucleotide 手法: EM (単粒子) / 解像度: 3.2 Å 48074 9ei2 EMDB-48074, PDB-9ei2: Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to an apo RECQL5 helicase (RECQL5 IRI Module focused-classified) 手法: EM (単粒子) / 解像度: 2.8 Å 48075 9ei3 EMDB-48075, PDB-9ei3: Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of AMPPNP 手法: EM (単粒子) / 解像度: 3.2 Å 48076 9ei4 EMDB-48076, PDB-9ei4: Cryo-EM structure of Human RNA polymerase II Elongation Complex bound to the RECQL5 helicase in the presence of ADP 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM
由来	homo sapiens (ヒト)
キーワード	TRANSCRIPTION / TRANSFERASE/DNA/RNA / translocation / Human RNA polymerase II / TRANSFERASE-DNA-RNA complex / RECQL5 helicase / IRI Module