EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dynamic molecular architecture and substrate recruitment of cullin3-RING E3 ligase CRL3.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 2, Page 336-350, Year 2024
掲載日	2024年2月8日
著者	Yuxia Hu / Zhao Zhang / Qiyu Mao / Xiang Zhang / Aihua Hao / Yu Xun / Yeda Wang / Lin Han / Wuqiang Zhan / Qianying Liu / Yue Yin / Chao Peng / Eva Marie Y Moresco / Zhenguo Chen / Bruce Beutler / Lei Sun /
PubMed 要旨	Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth factor-signaling and, frequently, oncogenesis. ...Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth factor-signaling and, frequently, oncogenesis. Cellular levels of the regulatory p85α subunit are tightly controlled by regulated proteasomal degradation. In adipose tissue and growth plates, failure of K48-linked p85α ubiquitination causes diabetes, lipodystrophy and dwarfism in mice, as in humans with SHORT syndrome. Here we elucidated the structures of the key ubiquitin ligase complexes regulating p85α availability. Specificity is provided by the substrate receptor KBTBD2, which recruits p85α to the cullin3-RING E3 ubiquitin ligase (CRL3). CRL3 forms multimers, which disassemble into dimers upon substrate binding (CRL3-p85α) and/or neddylation by the activator NEDD8 (CRL3~N8), leading to p85α ubiquitination and degradation. Deactivation involves dissociation of NEDD8 mediated by the COP9 signalosome and displacement of KBTBD2 by the inhibitor CAND1. The hereby identified structural basis of p85α regulation opens the way to better understanding disturbances of glucose regulation, growth and cancer.
リンク	Nat Struct Mol Biol / PubMed:38332366
手法	EM (単粒子)
解像度	3.76 - 12.18 Å
構造データ	34199 8gq6 EMDB-34199, PDB-8gq6: Cryo-EM Structure of the KBTBD2-CUL3-Rbx1 dimeric complex 手法: EM (単粒子) / 解像度: 3.96 Å 34449 8h33 EMDB-34449, PDB-8h33: Cryo-EM Structure of the KBTBD2-Cul3-Rbx1 tetrameric complex 手法: EM (単粒子) / 解像度: 7.86 Å 34450 8h34 EMDB-34450, PDB-8h34: Cryo-EM Structure of the KBTBD2-Cul3-Rbx1 hexameric complex 手法: EM (単粒子) / 解像度: 7.99 Å 34451 8h35 EMDB-34451, PDB-8h35: Cryo-EM Structure of the KBTBD2-Cul3-Rbx1 octameric complex 手法: EM (単粒子) / 解像度: 7.41 Å 34452 8h36 EMDB-34452, PDB-8h36: Cryo-EM Structure of the KBTBD2-CUL3-Rbx1-p85a dimeric complex 手法: EM (単粒子) / 解像度: 4.6 Å 34453 8h37 EMDB-34453, PDB-8h37: Cryo-EM Structure of the KBTBD2-CUL3-Rbx1-p85a tetrameric complex 手法: EM (単粒子) / 解像度: 7.52 Å EMDB-34454: local refinement of KBTBD2-p85a: KBTBD2-CRL3-p85a complex 手法: EM (単粒子) / 解像度: 4.11 Å 34455 8h38 EMDB-34455, PDB-8h38: Cryo-EM Structure of the KBTBD2-CRL3~N8-CSN(mutate) complex 手法: EM (単粒子) / 解像度: 4.25 Å EMDB-34456: local refinement of CSN1/2/4/5/6-CRL3/CTD-N8: KBTBD2-CRL3-CSN(mutate) complex 手法: EM (単粒子) / 解像度: 4.84 Å EMDB-34457: local refinement of CSN1/3/5/6/7/8: KBTBD2-CRL3-CSN(mutate) complex 手法: EM (単粒子) / 解像度: 4.21 Å EMDB-34458: local refinement of CSN5/6-CRL3/CTD-N8: KBTBD2-CRL3-CSN(mutate) complex 手法: EM (単粒子) / 解像度: 4.29 Å EMDB-34459: local refinement of KBTBD2-CRL3-CSN(mutate) complex 手法: EM (単粒子) / 解像度: 4.25 Å EMDB-34460: Cryo-EM Structure of the KBTBD2-CRL3~N8-CSN(mutate,no CSN3/8) complex 手法: EM (単粒子) / 解像度: 6.83 Å EMDB-34461: Cryo-EM Structure of the KBTBD2-CRL3~N8-CSN(mutate)-CSN(mutate,no CSN3/8) complex 手法: EM (単粒子) / 解像度: 8.05 Å 34462 8h3a EMDB-34462, PDB-8h3a: Cryo-EM Structure of the KBTBD2-CRL3~N8(removed)-CSN complex 手法: EM (単粒子) / 解像度: 7.51 Å EMDB-34463: Cryo-EM Structure of the KBTBD2-CRL3~N8(removed)-CSN(no CSN3/8) complex 手法: EM (単粒子) / 解像度: 7.15 Å EMDB-34466: Cryo-EM Structure of the KBTBD2-CRL3-CSN(no CSN3/8) complex 手法: EM (単粒子) / 解像度: 12.18 Å 34467 8h3f EMDB-34467, PDB-8h3f: Cryo-EM Structure of the KBTBD2-CRL3-CSN complex 手法: EM (単粒子) / 解像度: 6.73 Å 34473 8h3q EMDB-34473, PDB-8h3q: Cryo-EM Structure of the CAND1-Cul3-Rbx1 complex 手法: EM (単粒子) / 解像度: 3.76 Å 34474 8h3r EMDB-34474, PDB-8h3r: Cryo-EM Structure of the KBTBD2-CRL3~N8 dimeric complex 手法: EM (単粒子) / 解像度: 6.36 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	LIGASE (リガーゼ) / complex