+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-34473 | |||||||||
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Title | Cryo-EM Structure of the CAND1-Cul3-Rbx1 complex | |||||||||
Map data | ||||||||||
Sample |
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Keywords | ligase / complex | |||||||||
Function / homology | Function and homology information SCF complex assembly / liver morphogenesis / positive regulation of mitotic cell cycle phase transition / trophectodermal cellular morphogenesis / POZ domain binding / nuclear protein quality control by the ubiquitin-proteasome system / regulation protein catabolic process at postsynapse / polar microtubule / negative regulation of catalytic activity / COPII vesicle coating ...SCF complex assembly / liver morphogenesis / positive regulation of mitotic cell cycle phase transition / trophectodermal cellular morphogenesis / POZ domain binding / nuclear protein quality control by the ubiquitin-proteasome system / regulation protein catabolic process at postsynapse / polar microtubule / negative regulation of catalytic activity / COPII vesicle coating / anaphase-promoting complex-dependent catabolic process / stem cell division / cullin-RING-type E3 NEDD8 transferase / cellular response to chemical stress / NEDD8 transferase activity / cullin-RING ubiquitin ligase complex / RHOBTB3 ATPase cycle / embryonic cleavage / cell projection organization / positive regulation of mitotic metaphase/anaphase transition / Cul7-RING ubiquitin ligase complex / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling / positive regulation of protein autoubiquitination / protein neddylation / fibroblast apoptotic process / Notch binding / NEDD8 ligase activity / RHOBTB1 GTPase cycle / Cul5-RING ubiquitin ligase complex / negative regulation of response to oxidative stress / ubiquitin-ubiquitin ligase activity / negative regulation of Rho protein signal transduction / SCF ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / Cul4A-RING E3 ubiquitin ligase complex / mitotic metaphase chromosome alignment / negative regulation of type I interferon production / ubiquitin ligase complex scaffold activity / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / Cul4B-RING E3 ubiquitin ligase complex / Cul3-RING ubiquitin ligase complex / stress fiber assembly / positive regulation of cytokinesis / Prolactin receptor signaling / protein monoubiquitination / cullin family protein binding / positive regulation of RNA polymerase II transcription preinitiation complex assembly / sperm flagellum / endoplasmic reticulum to Golgi vesicle-mediated transport / RHOBTB2 GTPase cycle / protein autoubiquitination / protein K48-linked ubiquitination / Nuclear events stimulated by ALK signaling in cancer / ubiquitin ligase complex / gastrulation / TBP-class protein binding / positive regulation of TORC1 signaling / intrinsic apoptotic signaling pathway / post-translational protein modification / regulation of cellular response to insulin stimulus / cyclin binding / Regulation of BACH1 activity / T cell activation / positive regulation of protein ubiquitination / integrin-mediated signaling pathway / Degradation of DVL / cellular response to amino acid stimulus / Iron uptake and transport / Degradation of GLI1 by the proteasome / Recognition of DNA damage by PCNA-containing replication complex / Negative regulation of NOTCH4 signaling / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / protein destabilization / Vif-mediated degradation of APOBEC3G / Hedgehog 'on' state / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / DNA Damage Recognition in GG-NER / RING-type E3 ubiquitin transferase / negative regulation of canonical Wnt signaling pathway / Degradation of beta-catenin by the destruction complex / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / Dual Incision in GG-NER / Evasion by RSV of host interferon responses / mitotic spindle / Wnt signaling pathway / NOTCH1 Intracellular Domain Regulates Transcription / Regulation of expression of SLITs and ROBOs / spindle pole / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / Formation of Incision Complex in GG-NER / Interleukin-1 signaling / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Orc1 removal from chromatin Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.76 Å | |||||||||
Authors | Hu Y / Mao Q / Chen Z / Sun L | |||||||||
Funding support | China, 1 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2024 Title: Dynamic molecular architecture and substrate recruitment of cullin3-RING E3 ligase CRL3. Authors: Yuxia Hu / Zhao Zhang / Qiyu Mao / Xiang Zhang / Aihua Hao / Yu Xun / Yeda Wang / Lin Han / Wuqiang Zhan / Qianying Liu / Yue Yin / Chao Peng / Eva Marie Y Moresco / Zhenguo Chen / Bruce Beutler / Lei Sun / Abstract: Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth factor-signaling and, frequently, oncogenesis. ...Phosphatidylinositol 3-kinase α, a heterodimer of catalytic p110α and one of five regulatory subunits, mediates insulin- and insulin like growth factor-signaling and, frequently, oncogenesis. Cellular levels of the regulatory p85α subunit are tightly controlled by regulated proteasomal degradation. In adipose tissue and growth plates, failure of K48-linked p85α ubiquitination causes diabetes, lipodystrophy and dwarfism in mice, as in humans with SHORT syndrome. Here we elucidated the structures of the key ubiquitin ligase complexes regulating p85α availability. Specificity is provided by the substrate receptor KBTBD2, which recruits p85α to the cullin3-RING E3 ubiquitin ligase (CRL3). CRL3 forms multimers, which disassemble into dimers upon substrate binding (CRL3-p85α) and/or neddylation by the activator NEDD8 (CRL3~N8), leading to p85α ubiquitination and degradation. Deactivation involves dissociation of NEDD8 mediated by the COP9 signalosome and displacement of KBTBD2 by the inhibitor CAND1. The hereby identified structural basis of p85α regulation opens the way to better understanding disturbances of glucose regulation, growth and cancer. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_34473.map.gz | 57.1 MB | EMDB map data format | |
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Header (meta data) | emd-34473-v30.xml emd-34473.xml | 19.4 KB 19.4 KB | Display Display | EMDB header |
Images | emd_34473.png | 81 KB | ||
Filedesc metadata | emd-34473.cif.gz | 7 KB | ||
Others | emd_34473_half_map_1.map.gz emd_34473_half_map_2.map.gz | 49.7 MB 49.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-34473 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34473 | HTTPS FTP |
-Validation report
Summary document | emd_34473_validation.pdf.gz | 675.8 KB | Display | EMDB validaton report |
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Full document | emd_34473_full_validation.pdf.gz | 675.4 KB | Display | |
Data in XML | emd_34473_validation.xml.gz | 12.2 KB | Display | |
Data in CIF | emd_34473_validation.cif.gz | 14.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-34473 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-34473 | HTTPS FTP |
-Related structure data
Related structure data | 8h3qMC 8gq6C 8h33C 8h34C 8h35C 8h36C 8h37C 8h38C 8h3aC 8h3fC 8h3rC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_34473.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.044 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34473_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34473_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : CAND1-Cul3-Rbx1 complex
Entire | Name: CAND1-Cul3-Rbx1 complex |
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Components |
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-Supramolecule #1: CAND1-Cul3-Rbx1 complex
Supramolecule | Name: CAND1-Cul3-Rbx1 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: Homo sapiens (human) |
-Supramolecule #2: CAND1
Supramolecule | Name: CAND1 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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-Supramolecule #3: Cul3, Rbx1
Supramolecule | Name: Cul3, Rbx1 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#3 |
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-Macromolecule #1: Cullin-associated NEDD8-dissociated protein 1
Macromolecule | Name: Cullin-associated NEDD8-dissociated protein 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 136.529297 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MASASYHISN LLEKMTSSDK DFRFMATNDL MTELQKDSIK LDDDSERKVV KMILKLLEDK NGEVQNLAVK CLGPLVSKVK EYQVETIVD TLCTNMLSDK EQLRDISSIG LKTVIGELPP ASSGSALAAN VCKKITGRLT SAIAKQEDVS VQLEALDIMA D MLSRQGGL ...String: MASASYHISN LLEKMTSSDK DFRFMATNDL MTELQKDSIK LDDDSERKVV KMILKLLEDK NGEVQNLAVK CLGPLVSKVK EYQVETIVD TLCTNMLSDK EQLRDISSIG LKTVIGELPP ASSGSALAAN VCKKITGRLT SAIAKQEDVS VQLEALDIMA D MLSRQGGL LVNFHPSILT CLLPQLTSPR LAVRKRTIIA LGHLVMSCGN IVFVDLIEHL LSELSKNDSM STTRTYIQCI AA ISRQAGH RIGEYLEKII PLVVKFCNVD DDELREYCIQ AFESFVRRCP KEVYPHVSTI INICLKYLTY DPNYNYDDED EDE NAMDAD GGDDDDQGSD DEYSDDDDMS WKVRRAAAKC LDAVVSTRHE MLPEFYKTVS PALISRFKER EENVKADVFH AYLS LLKQT RPVQSWLCDP DAMEQGETPL TMLQSQVPNI VKALHKQMKE KSVKTRQCCF NMLTELVNVL PGALTQHIPV LVPGI IFSL NDKSSSSNLK IDALSCLYVI LCNHSPQVFH PHVQALVPPV VACVGDPFYK ITSEALLVTQ QLVKVIRPLD QPSSFD ATP YIKDLFTCTI KRLKAADIDQ EVKERAISCM GQIICNLGDN LGSDLPNTLQ IFLERLKNEI TRLTTVKALT LIAGSPL KI DLRPVLGEGV PILASFLRKN QRALKLGTLS ALDILIKNYS DSLTAAMIDA VLDELPPLIS ESDMHVSQMA ISFLTTLA K VYPSSLSKIS GSILNELIGL VRSPLLQGGA LSAMLDFFQA LVVTGTNNLG YMDLLRMLTG PVYSQSTALT HKQSYYSIA KCVAALTRAC PKEGPAVVGQ FIQDVKNSRS TDSIRLLALL SLGEVGHHID LSGQLELKSV ILEAFSSPSE EVKSAASYAL GSISVGNLP EYLPFVLQEI TSQPKRQYLL LHSLKEIISS ASVVGLKPYV ENIWALLLKH CECAEEGTRN VVAECLGKLT L IDPETLLP RLKGYLISGS SYARSSVVTA VKFTISDHPQ PIDPLLKNCI GDFLKTLEDP DLNVRRVALV TFNSAAHNKP SL IRDLLDT VLPHLYNETK VRKELIREVE MGPFKHTVDD GLDIRKAAFE CMYTLLDSCL DRLDIFEFLN HVEDGLKDHY DIK MLTFLM LVRLSTLCPS AVLQRLDRLV EPLRATCTTK VKANSVKQEF EKQDELKRSA MRAVAALLTI PEAEKSPLMS EFQS QISSN PELAAIFESI QKDSSSTNLE SMDTS UniProtKB: Cullin-associated NEDD8-dissociated protein 1 |
-Macromolecule #2: Cullin-3
Macromolecule | Name: Cullin-3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 89.063328 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MSNLSKGTGS RKDTKMRIRA FPMTMDEKYV NSIWDLLKNA IQEIQRKNNS GLSFEELYRN AYTMVLHKHG EKLYTGLREV VTEHLINKV REDVLNSLNN NFLQTLNQAW NDHQTAMVMI RDILMYMDRV YVQQNNVENV YNLGLIIFRD QVVRYGCIRD H LRQTLLDM ...String: MSNLSKGTGS RKDTKMRIRA FPMTMDEKYV NSIWDLLKNA IQEIQRKNNS GLSFEELYRN AYTMVLHKHG EKLYTGLREV VTEHLINKV REDVLNSLNN NFLQTLNQAW NDHQTAMVMI RDILMYMDRV YVQQNNVENV YNLGLIIFRD QVVRYGCIRD H LRQTLLDM IARERKGEVV DRGAIRNACQ MLMILGLEGR SVYEEDFEAP FLEMSAEFFQ MESQKFLAEN SASVYIKKVE AR INEEIER VMHCLDKSTE EPIVKVVERE LISKHMKTIV EMENSGLVHM LKNGKTEDLG CMYKLFSRVP NGLKTMCECM SSY LREQGK ALVSEEGEGK NPVDYIQGLL DLKSRFDRFL LESFNNDRLF KQTIAGDFEY FLNLNSRSPE YLSLFIDDKL KKGV KGLTE QEVETILDKA MVLFRFMQEK DVFERYYKQH LARRLLTNKS VSDDSEKNMI SKLKTECGCQ FTSKLEGMFR DMSIS NTTM DEFRQHLQAT GVSLGGVDLT VRVLTTGYWP TQSATPKCNI PPAPRHAFEI FRRFYLAKHS GRQLTLQHHM GSADLN ATF YGPVKKEDGS EVGVGGAQVT GSNTRKHILQ VSTFQMTILM LFNNREKYTF EEIQQETDIP ERELVRALQS LACGKPT QR VLTKEPKSKE IENGHIFTVN DQFTSKLHRV KIQTVAAKQG ESDPERKETR QKVDDDRKHE IEAAIVRIMK SRKKMQHN V LVAEVTQQLK ARFLPSPVVI KKRIEGLIER EYLARTPEDR KVYTYVA UniProtKB: Cullin-3 |
-Macromolecule #3: E3 ubiquitin-protein ligase RBX1
Macromolecule | Name: E3 ubiquitin-protein ligase RBX1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 12.289977 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MAAAMDVDTP SGTNSGAGKK RFEVKKWNAV ALWAWDIVVD NCAICRNHIM DLCIECQANQ ASATSEECTV AWGVCNHAFH FHCISRWLK TRQVCPLDNR EWEFQKYGH UniProtKB: E3 ubiquitin-protein ligase RBX1 |
-Macromolecule #4: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 4 / Number of copies: 3 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 53.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.76 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 644994 |
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |