EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Columnar structure of human telomeric chromatin.
ジャーナル・号・ページ	Nature, Vol. 609, Issue 7929, Page 1048-1055, Year 2022
掲載日	2022年9月14日
著者	Aghil Soman / Sook Yi Wong / Nikolay Korolev / Wahyu Surya / Simon Lattmann / Vinod K Vogirala / Qinming Chen / Nikolay V Berezhnoy / John van Noort / Daniela Rhodes / Lars Nordenskiöld /
PubMed 要旨	Telomeres, the ends of eukaryotic chromosomes, play pivotal parts in ageing and cancer and are targets of DNA damage and the DNA damage response. Little is known about the structure of telomeric ...Telomeres, the ends of eukaryotic chromosomes, play pivotal parts in ageing and cancer and are targets of DNA damage and the DNA damage response. Little is known about the structure of telomeric chromatin at the molecular level. Here we used negative stain electron microscopy and single-molecule magnetic tweezers to characterize 3-kbp-long telomeric chromatin fibres. We also obtained the cryogenic electron microscopy structure of the condensed telomeric tetranucleosome and its dinucleosome unit. The structure displayed close stacking of nucleosomes with a columnar arrangement, and an unusually short nucleosome repeat length that comprised about 132 bp DNA wound in a continuous superhelix around histone octamers. This columnar structure is primarily stabilized by the H2A carboxy-terminal and histone amino-terminal tails in a synergistic manner. The columnar conformation results in exposure of the DNA helix, which may make it susceptible to both DNA damage and the DNA damage response. The conformation also exists in an alternative open state, in which one nucleosome is unstacked and flipped out, which exposes the acidic patch of the histone surface. The structural features revealed in this work suggest mechanisms by which protein factors involved in telomere maintenance can access telomeric chromatin in its compact form.
リンク	Nature / PubMed:36104563
手法	EM (単粒子)
解像度	3.5 - 14.0 Å
構造データ	31806 7v90 EMDB-31806, PDB-7v90: Telomeric mononucleosome 手法: EM (単粒子) / 解像度: 3.5 Å 31810 7v96 EMDB-31810, PDB-7v96: Telomeric Dinucleosome 手法: EM (単粒子) / 解像度: 3.92 Å 31815 7v9c EMDB-31815, PDB-7v9c: Telomeric Dinucleosome in open state 手法: EM (単粒子) / 解像度: 4.5 Å 31816 7v9j EMDB-31816, PDB-7v9j: Telomeric trinucleosome 手法: EM (単粒子) / 解像度: 8.0 Å 31823 7v9k EMDB-31823, PDB-7v9k: Telomeric tetranucleosome 手法: EM (単粒子) / 解像度: 8.1 Å 31826 7v9s EMDB-31826, PDB-7v9s: Telomeric trinucleosome in open state 手法: EM (単粒子) / 解像度: 11.0 Å 31832 7va4 EMDB-31832, PDB-7va4: Telomeric tetranucleosome in open state 手法: EM (単粒子) / 解像度: 14.0 Å EMDB-31907: Telomeric Dinucleosome at 4.6 angstroms 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-31908: Telomeric Dinucleosome at 5angstroms 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-31909: Open dinucleosome at 6.6 angstroms 手法: EM (単粒子) / 解像度: 6.7 Å
由来	homo sapiens (ヒト)
キーワード	DNA BINDING PROTEIN/DNA / Telomere / nucleosome / chromatin / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex