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-Structure paper
タイトル | High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 4052, Year 2023 |
掲載日 | 2023年7月8日 |
著者 | Fenglin Li / Chun-Feng David Hou / Ravi K Lokareddy / Ruoyu Yang / Francesca Forti / Federica Briani / Gino Cingolani / |
PubMed 要旨 | E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after ...E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4. |
リンク | Nat Commun / PubMed:37422479 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 3.96 Å |
構造データ | EMDB-28280, PDB-8env: EMDB-28405, PDB-8eon: EMDB-29406, PDB-8frs: EMDB-29481, PDB-8fuv: EMDB-29486, PDB-8fvg: EMDB-29487, PDB-8fvh: |
由来 |
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キーワード | VIRUS / Pseudomonas / phage / baseplate / E217 / capsid / decorating proteins |