EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.
ジャーナル・号・ページ	Science, Vol. 375, Issue 6586, Page 1287-1291, Year 2022
掲載日	2022年3月18日
著者	Christopher W Koo / Frank J Tucci / Yuan He / Amy C Rosenzweig /
PubMed 要旨	Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using ...Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.
リンク	Science / PubMed:35298269 / PubMed Central
手法	EM (単粒子)
解像度	2.14 - 3.65 Å
構造データ	24826 7s4h EMDB-24826, PDB-7s4h: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.14 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.14 Å 24827 7s4i EMDB-24827, PDB-7s4i: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.26 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.26 Å 24828 7s4j EMDB-24828, PDB-7s4j: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.16 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.16 Å 24829 7s4k EMDB-24829, PDB-7s4k: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.34 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.36 Å 24830 7s4l EMDB-24830, PDB-7s4l: CryoEM structure of Methylotuvimicrobium alcaliphilum 20Z pMMO in a POPC nanodisc at 2.46 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.46 Å 24831 7s4m EMDB-24831, PDB-7s4m: CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution 手法: EM (単粒子) / 解像度: 2.42 Å 25683 7t4o EMDB-25683, PDB-7t4o: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide in a native lipid nanodisc at 3.65 Angstrom resolution 手法: EM (単粒子) / 解像度: 3.65 Å 25684 7t4p EMDB-25684: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 angstrom resolution PDB-7t4p: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 Angstrom resolution 手法: EM (単粒子) / 解像度: 3.62 Å
化合物	ChemComp-CU: COPPER (II) ION ChemComp-D10: DECANE / デカン ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / O-(1-O,2-O-ジドデカノイル-L-グリセロ-3-ホスホ)コリン / リン脂質YM ChemComp-HXG: 1,2-dihexanoyl-sn-glycero-3-phosphocholine / O-(1-O,2-O-ジヘキサノイル-L-グリセロ-3-ホスホ)コリン ChemComp-P1O: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / ジデカノイルホスファチジルコリン / DDPC, リン脂質YM ChemComp-HOH: WATER ChemComp-6ER: (S)-2,3-bis(hexanoyloxy)propyl(2-(trimethylammonio)ethyl)phosphate ChemComp-D12: DODECANE / ドデカン
由来	Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) (バクテリア) methylococcus capsulatus str. bath (バクテリア) Methylotuvimicrobium alcaliphilum 20Z (バクテリア) methylomicrobium alcaliphilum (strain dsm 19304 / ncimb 14124 / vkm b-2133 / 20z) (バクテリア) methylocystis sp. atcc 49242 (バクテリア)
キーワード	OXIDOREDUCTASE / Complex