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-Structure paper
タイトル | Nucleolar maturation of the human small subunit processome. |
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ジャーナル・号・ページ | Science, Vol. 373, Issue 6560, Page eabj5338, Year 2021 |
掲載日 | 2021年9月10日 |
著者 | Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge / |
PubMed 要旨 | The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within. |
リンク | Science / PubMed:34516797 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.23 - 6.37 Å |
構造データ | EMDB-23936, PDB-7mq8: EMDB-23937, PDB-7mq9: EMDB-23938, PDB-7mqa: EMDB-23939: EMDB-23940: EMDB-24149: EMDB-24150: EMDB-24151: EMDB-24152: EMDB-24153: EMDB-24154: EMDB-24155: EMDB-24156: EMDB-24157: EMDB-24158: EMDB-24159: EMDB-24160: EMDB-24161: EMDB-24162: EMDB-24163: EMDB-24164: EMDB-24165: EMDB-24166: EMDB-24167: EMDB-24168: EMDB-24169: EMDB-24170: EMDB-24171: EMDB-24172: EMDB-24173: EMDB-24174: EMDB-24175: EMDB-24176: PDB-7mqj: |
化合物 | ChemComp-MG: ChemComp-ATP: ChemComp-ZN: ChemComp-GTP: ChemComp-SAH: ChemComp-ADP: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / Ribosomal assembly intermediate / HYDROLASE / Helicase / Ribosome Assembly |