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-Structure paper
| タイトル | Tail proteins of phage SU10 reorganize into the nozzle for genome delivery. |
|---|---|
| ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 5622, Year 2022 |
| 掲載日 | 2022年9月24日 |
 著者 | Marta Šiborová / Tibor Füzik / Michaela Procházková / Jiří Nováček / Martin Benešík / Anders S Nilsson / Pavel Plevka /   |
| PubMed 要旨 | Escherichia coli phage SU10 belongs to the genus Kuravirus from the class Caudoviricetes of phages with short non-contractile tails. In contrast to other short-tailed phages, the tails of Kuraviruses ...Escherichia coli phage SU10 belongs to the genus Kuravirus from the class Caudoviricetes of phages with short non-contractile tails. In contrast to other short-tailed phages, the tails of Kuraviruses elongate upon cell attachment. Here we show that the virion of SU10 has a prolate head, containing genome and ejection proteins, and a tail, which is formed of portal, adaptor, nozzle, and tail needle proteins and decorated with long and short fibers. The binding of the long tail fibers to the receptors in the outer bacterial membrane induces the straightening of nozzle proteins and rotation of short tail fibers. After the re-arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that extends the tail by 28 nm. Subsequently, the tail needle detaches from the nozzle proteins and five types of ejection proteins are released from the SU10 head. The nozzle with the putative extension formed by the ejection proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. It is likely that this mechanism of genome delivery, involving the formation of the tail nozzle, is employed by all Kuraviruses. |
 リンク | Nat Commun / PubMed:36153309 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.4 - 10.0 Å |
| 構造データ | EMDB-14483, PDB-7z44: EMDB-14484, PDB-7z45: EMDB-14485, PDB-7z46: EMDB-14486, PDB-7z47: EMDB-14487, PDB-7z48: EMDB-14488, PDB-7z49: EMDB-14489, PDB-7z4a:  EMDB-14490: Capsid of genome release intermediate of bacteriophage SU10  EMDB-14491: Assymetric reconstruction of bacteriophage SU10 genome release intermediate capsid cap EMDB-14492, PDB-7z4b: EMDB-14495, PDB-7z4f:  EMDB-14909: Tail needle of bacteriophage SU10  EMDB-14920: Bacteriophage SU10 - genome release intermediate, composite map |
| 由来 |
|
 キーワード | VIRUS / bacteriophage / portal / tail / base plate / nozzle / tail fibers / capsid / major capsid protein / asymmetric |
escherichia phage vb_ecop_su10 (ファージ)
Escherichia coli (大腸菌)