+検索条件
-Structure paper
タイトル | Shulin packages axonemal outer dynein arms for ciliary targeting. |
---|---|
ジャーナル・号・ページ | Science, Vol. 371, Issue 6532, Page 910-916, Year 2021 |
掲載日 | 2021年2月26日 |
著者 | Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter / |
PubMed 要旨 | The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia. |
リンク | Science / PubMed:33632841 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.3 - 9.32 Å |
構造データ | EMDB-11576, PDB-6zyw: EMDB-11577: EMDB-11578: EMDB-11579, PDB-6zyx: EMDB-11580: EMDB-11581, PDB-6zyy: EMDB-11582: EMDB-11583: EMDB-11584: |
化合物 | ChemComp-ADP: ChemComp-ATP: ChemComp-GTP: |
由来 |
|
キーワード | MOTOR PROTEIN / Cilia / dynein / microtubules / motor / Complex |