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-Structure paper
タイトル | ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 120, Year 2021 |
掲載日 | 2021年1月5日 |
著者 | Alexander Mühleip / Rasmus Kock Flygaard / Jana Ovciarikova / Alice Lacombe / Paula Fernandes / Lilach Sheiner / Alexey Amunts / |
PubMed 要旨 | Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has ...Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has been observed, but its structural basis is unknown. Here, we report that the apicomplexan ATP synthase assembles into cyclic hexamers, essential to shape their distinct cristae. Cryo-EM was used to determine the structure of the hexamer, which is held together by interactions between parasite-specific subunits in the lumenal region. Overall, we identified 17 apicomplexan-specific subunits, and a minimal and nuclear-encoded subunit-a. The hexamer consists of three dimers with an extensive dimer interface that includes bound cardiolipins and the inhibitor IF. Cryo-ET and subtomogram averaging revealed that hexamers arrange into ~20-megadalton pentagonal pyramids in the curved apical membrane regions. Knockout of the linker protein ATPTG11 resulted in the loss of pentagonal pyramids with concomitant aberrantly shaped cristae. Together, this demonstrates that the unique macromolecular arrangement is critical for the maintenance of cristae morphology in Apicomplexa. |
リンク | Nat Commun / PubMed:33402698 / PubMed Central |
手法 | EM (単粒子) / EM (サブトモグラム平均) |
解像度 | 2.8 - 34.0 Å |
構造データ | EMDB-10520: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region map EMDB-10521: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring map EMDB-10522: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, peripheral stalk map EMDB-10523: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, rotor-stator map EMDB-10524: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, consensus map EMDB-10525: Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, membrane region EMDB-10526: EMDB-10527: EMDB-11403: |
化合物 | ChemComp-PC1: ChemComp-LMT: ChemComp-CDL: ChemComp-PEE: ChemComp-ATP: ChemComp-MG: ChemComp-ADP: |
由来 |
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キーワード | MEMBRANE PROTEIN / mitochondrial / ATP synthase / membrane region / lipids / F1 / c-ring / peripheral stalk / OSCP / rotor / stator / dimer / hexamer |