EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.
ジャーナル・号・ページ	Science, Vol. 376, Issue 6595, Page 844-852, Year 2022
掲載日	2022年5月20日
著者	Stanislau Yatskevich / Kyle W Muir / Dom Bellini / Ziguo Zhang / Jing Yang / Thomas Tischer / Masa Predin / Tom Dendooven / Stephen H McLaughlin / David Barford /
PubMed 要旨	Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron ...Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle.
リンク	Science / PubMed:35420891 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.44 - 12.0 Å
構造データ	13437 7pii EMDB-13437, PDB-7pii: Structure of the human CCAN CENP-A alpha-satellite complex 手法: EM (単粒子) / 解像度: 2.68 Å 13473 7pkn EMDB-13473, PDB-7pkn: Structure of the human CCAN deltaCT complex 手法: EM (単粒子) / 解像度: 3.2 Å 14334 7r5r EMDB-14334, PDB-7r5r: Structure of the human CCAN CENP-A alpha-satellite complex 手法: EM (単粒子) / 解像度: 2.44 Å 14336 7r5s EMDB-14336, PDB-7r5s: Structure of the human CCAN bound to alpha satellite DNA 手法: EM (単粒子) / 解像度: 2.83 Å 14341 7r5v EMDB-14341, PDB-7r5v: Structure of the human CCAN CENP-A alpha-satellite complex 手法: EM (単粒子) / 解像度: 4.55 Å 14351 7ywx EMDB-14351, PDB-7ywx: Structure of the human CCAN CENP-A alpha-satellite complex 手法: EM (単粒子) / 解像度: 12.0 Å 14375 7yyh EMDB-14375, PDB-7yyh: Structure of the human CCANdeltaT CENP-A alpha-satellite complex 手法: EM (単粒子) / 解像度: 8.9 Å PDB-7pb4: Cenp-HIK 3-protein complex 手法: X-RAY DIFFRACTION / 解像度: 2.49 Å PDB-7pb8: Crystal structure of the CENP-OPQUR complex 手法: X-RAY DIFFRACTION / 解像度: 3.68 Å
化合物	ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	CELL CYCLE (細胞周期) / inner kinetochore / Kinetochore (動原体) / chromosome segregation / centromere (セントロメア) / Chromosome (染色体) / cell division (細胞分裂)