EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An atomic structure of the human 26S proteasome.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 23, Issue 9, Page 778-785, Year 2016
掲載日	2016年7月18日
著者	Xiuliang Huang / Bai Luan / Jianping Wu / Yigong Shi /
PubMed 要旨	We report the cryo-EM structure of the human 26S proteasome at an average resolution of 3.5 Å, allowing atomic modeling of 28 subunits in the core particle (CP) and 18 subunits in the regulatory ...We report the cryo-EM structure of the human 26S proteasome at an average resolution of 3.5 Å, allowing atomic modeling of 28 subunits in the core particle (CP) and 18 subunits in the regulatory particle (RP). The C-terminal residues of Rpt3 and Rpt5 subunits in the RP can be seen inserted into surface pockets formed between adjacent α subunits in the CP. Each of the six Rpt subunits contains a bound nucleotide, and the central gate of the CP α-ring is closed despite RP association. The six pore 1 loops in the Rpt ring are arranged similarly to a spiral staircase along the axial channel of substrate transport, which is constricted by the pore 2 loops. We also determined the cryo-EM structure of the human proteasome bound to the deubiquitinating enzyme USP14 at 4.35-Å resolution. Together, our structures provide a framework for mechanistic understanding of eukaryotic proteasome function.
リンク	Nat Struct Mol Biol / PubMed:27428775
手法	EM (単粒子)
解像度	3.5 - 4.6 Å
構造データ	EMDB-9507: Cryo-EM map of the RP region of human 26S proteasome at 4.3A 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-9508: Cryo-EM map of the human 26S proteasome with C1 symmetry 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-9509: Cryo-EM map of the RP region (Class1) of human 26S proteasome 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-9510: Cryo-EM map of the RP region (Class2) of human 26S proteasome 手法: EM (単粒子) / 解像度: 4.6 Å 9511 5gjq EMDB-9511: Cryo-EM map of the human 26S proteasome bound to USP14_UbAl PDB-5gjq: Structure of the human 26S proteasome bound to USP14-UbAl 手法: EM (単粒子) / 解像度: 4.35 Å 9512 5gjr EMDB-9512: Cryo-EM map of the human 26S proteasome at 3.5A resolution with C2 symmetry PDB-5gjr: An atomic structure of the human 26S proteasome 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM / アデノシン二リン酸
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	HYDROLASE (加水分解酵素) / protein complex (タンパク質複合体) / human proteasome (プロテアソーム)