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TitleThree-dimensional structures of Vibrio cholerae typing podophage VP1 in two states.
Journal, issue, pagesStructure, Vol. 32, Issue 12, Page 2364-22374.e2, Year 2024
Publish dateDec 5, 2024
AuthorsHao Pang / Fenxia Fan / Jing Zheng / Hao Xiao / Zhixue Tan / Jingdong Song / Biao Kan / Hongrong Liu /
PubMed AbstractLytic podophages (VP1-VP5) play crucial roles in subtyping Vibrio cholerae O1 biotype El Tor. However, until now no structures of these phages have been available, which hindered our understanding of ...Lytic podophages (VP1-VP5) play crucial roles in subtyping Vibrio cholerae O1 biotype El Tor. However, until now no structures of these phages have been available, which hindered our understanding of the molecular mechanisms of infection and DNA release. Here, we determined the cryoelectron microscopy (cryo-EM) structures of mature and DNA-ejected VP1 structures at near-atomic and subnanometer resolutions, respectively. The VP1 head is composed of 415 copies of the major capsid protein gp7 and 11 turret-shaped spikes. The VP1 tail consists of an adapter, a nozzle, a slender ring, and a tail needle, and is flanked by three extended fibers I and six trimeric fibers II. Conformational changes of fiber II in DNA-ejected VP1 may cause the release of the tail needle and core proteins, forming an elongated tail channel. Our structures provide insights into the molecular mechanisms of infection and DNA release for podophages with a tail needle.
External linksStructure / PubMed:39471801
MethodsEM (single particle)
Resolution2.64 - 6.7 Å
Structure data

60197

8zkk

EMDB-60197, PDB-8zkk:
Portal-tail of Vibrio cholerae typing phage mature VP1
Method: EM (single particle) / Resolution: 3.6 Å

60199

8zkm

EMDB-60199, PDB-8zkm:
portal-tail of Vibrio cholerae typing phage release VP1
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-60230: capsid of Vivrio cholerae typing phage VP1
Method: EM (single particle) / Resolution: 2.64 Å

EMDB-60673: Release tail of Vibrio cholerae typing phage VP1
Method: EM (single particle) / Resolution: 4.65 Å

60702

9in6

EMDB-60702, PDB-9in6:
Capsid of Vibrio cholerae phage mature VP1
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • vibrio cholerae (bacteria)
KeywordsVIRAL PROTEIN / phage / virus / vibrio cholera phage

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