EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation.
ジャーナル・号・ページ	Dev Cell, Year 2024
掲載日	2024年9月23日
著者	Yixin Xu / Hugo Muñoz-Hernández / Rościsław Krutyhołowa / Florina Marxer / Ferdane Cetin / Michal Wieczorek /
PubMed 要旨	Microtubule nucleation is templated by the γ-tubulin ring complex (γ-TuRC), but its structure deviates from the geometry of α-/β-tubulin in the microtubule, explaining the complex's poor ...Microtubule nucleation is templated by the γ-tubulin ring complex (γ-TuRC), but its structure deviates from the geometry of α-/β-tubulin in the microtubule, explaining the complex's poor nucleating activity. Several proteins may activate the γ-TuRC, but the mechanisms underlying activation are not known. Here, we determined the structure of the porcine γ-TuRC purified using CDK5RAP2's centrosomin motif 1 (CM1). We identified an unexpected conformation of the γ-TuRC bound to multiple protein modules containing MZT2, GCP2, and CDK5RAP2, resulting in a long-range constriction of the γ-tubulin ring that brings it in closer agreement with the 13-protofilament microtubule. Additional CDK5RAP2 promoted γ-TuRC decoration and stimulated the microtubule-nucleating activities of the porcine γ-TuRC and a reconstituted, CM1-free human complex in single-molecule assays. Our results provide a structural mechanism for the control of microtubule nucleation by CM1 proteins and identify conformational transitions in the γ-TuRC that prime it for microtubule nucleation.
リンク	Dev Cell / PubMed:39321808
手法	EM (単粒子)
解像度	4.0 - 7.5 Å
構造データ	EMDB-51007: Structure of the Position 1 to 3 Open gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-51008: Structure of the Position 4 to 11 Open gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.37 Å EMDB-51010: Structure of the Position 10 to 12 Open gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-51011: Structure of the Position 1 to 3 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 7.5 Å EMDB-51012: Structure of the Position 4 to 7 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-51013: Structure of the Position 13 to 14 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 7.5 Å EMDB-51014: Structure of the Position 10 to 12 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-51015: Structure of the Position 8 to 9 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-51016: Consensus cryo-EM reconstruction of the S. scrofa gamma-TuRC 手法: EM (単粒子) / 解像度: 4.0 Å 51017 9g3x EMDB-51017, PDB-9g3x: Structure of the Partially-assembled gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.5 Å 51018 9g3y EMDB-51018, PDB-9g3y: Structure of the Native CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 6.8 Å 51019 9g3z EMDB-51019, PDB-9g3z: Structure of the Open gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.3 Å 51020 9g40 EMDB-51020, PDB-9g40: Structure of the Position 7 CMG-decorated gamma-Tubulin Ring Complex from Pig Brain 手法: EM (単粒子) / 解像度: 4.3 Å
由来	sus scrofa (ブタ) homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN / Tubulin Complex