EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 16, Page 2911-2924.e16, Year 2023
掲載日	2023年8月17日
著者	Morgan L Jones / Valentina Aria / Yasemin Baris / Joseph T P Yeeles /
PubMed 要旨	During eukaryotic DNA replication, Pol α-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides during discontinuous lagging-strand ...During eukaryotic DNA replication, Pol α-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides during discontinuous lagging-strand replication. How Pol α-primase is targeted to replication forks to prime DNA synthesis is not fully understood. Here, by determining cryoelectron microscopy (cryo-EM) structures of budding yeast and human replisomes containing Pol α-primase, we reveal a conserved mechanism for the coordination of priming by the replisome. Pol α-primase binds directly to the leading edge of the CMG (CDC45-MCM-GINS) replicative helicase via a complex interaction network. The non-catalytic PRIM2/Pri2 subunit forms two interfaces with CMG that are critical for in vitro DNA replication and yeast cell growth. These interactions position the primase catalytic subunit PRIM1/Pri1 directly above the exit channel for lagging-strand template single-stranded DNA (ssDNA), revealing why priming occurs efficiently only on the lagging-strand template and elucidating a mechanism for Pol α-primase to overcome competition from RPA to initiate primer synthesis.
リンク	Mol Cell / PubMed:37506699 / PubMed Central
手法	EM (単粒子)
解像度	2.98 - 8.75098 Å
構造データ	EMDB-15303: S. cerevisiae pol alpha bound to the core replisome engaged with a fork DNA substrate containing a 60 nucleotide lagging strand - unbinned. 手法: EM (単粒子) / 解像度: 3.34 Å EMDB-15304: Csm3/Tof1 local refinement - pol alpha bound replisome 手法: EM (単粒子) / 解像度: 3.93 Å EMDB-15305: C-tier local refinement, pol alpha - replisome complex. Locally filtered 手法: EM (単粒子) / 解像度: 3.23 Å EMDB-15306: Pol12, PolA1 and Pri2 N-terminal domain local refinement. Pol alpha - replisome complex. 手法: EM (単粒子) / 解像度: 5.04 Å 15309 8b9a 8b9b EMDB-15309: Pol alpha - replisome complex containing Ctf4. Engaged on a DNA fork containing a 60 nucleotide lagging strand. PDB-8b9a: S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. PDB-8b9b: S. cerevisiae replisome + Ctf4, bound by pol alpha. Complex engaged with a fork DNA substrate containing a 60 nucleotide lagging strand. 手法: EM (単粒子) / 解像度: 4.64 Å EMDB-15310: Ctf4 - GINS - Cdc45 local refinement. Pol alpha - replisome complex. 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-15340: MCM C-tier local refinement. Human replisome bound by pol alpha, engaged on fork DNA with 60 nt lagging strand. 手法: EM (単粒子) / 解像度: 3.7 Å 15341 8b9d EMDB-15341: Human replisome bound by pol alpha, engaged on fork DNA containing a 60 nt lagging strand. PDB-8b9d: Human replisome bound by Pol Alpha 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-15342: AND-1, CDC45, GINS local refinement. Human replisome bound by pol alpha - engaged on a 60 nt lagging strand DNA fork substrate. 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-15349: Human replisome bound by Pol Alpha, locally filtered. Strong PRIM1 density. Engaged on a fork DNA substrate containing a 60 nt lagging strand. 手法: EM (単粒子) / 解像度: 4.33 Å EMDB-15356: TIMELESS/TIPIN local refinement. Human replisome bound by Pol Alpha engaged on fork DNA with a 60 nt lagging strand. 手法: EM (単粒子) / 解像度: 4.05 Å EMDB-15902: S. cerevisiae replisome + Ctf4 + pol alpha. Unbinned, locally filtered. 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-15904: Composite map - human replisome + Pol alpha 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-15918: Human replisome + pol alpha. Not engaged on DNA. 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-15922: Human replisome bound to pol alpha engaged on a DNA fork substrate containing a 15 nucleotide lagging strand. 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-15923: Human replisome prepared in the presence of a fork DNA substrate containing a 60 nucleotide lagging strand. Off DNA. 手法: EM (単粒子) / 解像度: 3.6 Å 15924 8b9c EMDB-15924, PDB-8b9c: S. cerevisiae pol alpha - replisome complex 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-16247: Local refinement, Pri1:Pri2-CTD. Pol alpha primase associated budding yeast replisome. 手法: EM (単粒子) / 解像度: 8.75098 Å EMDB-16248: Pol alpha primase associated budding yeast replisome. Consensus refinement containing clear density for the Pri2-CTD and Pol1 exo/cat domains. 手法: EM (単粒子) / 解像度: 4.61857 Å EMDB-16320: Pol-alpha primase associated replisome binned consensus refinement in the absence of Ctf4, 60 nucleotide 5'-flap DNA fork 手法: EM (単粒子) / 解像度: 4.64 Å EMDB-16322: Pol alpha-primase associated replisome unbinned consensus refinement in the absence of Ctf4, 60 nucleotide 5'-flap DNA fork 手法: EM (単粒子) / 解像度: 3.47 Å EMDB-16323: Un-binned consensus refinement of Pol alpha-primase associated with the replisome only via the Pri2:Mcm5ZnF interface, 60 nucleotide 5'-flap DNA fork 手法: EM (単粒子) / 解像度: 2.98 Å EMDB-16885: Pol12:Pol1CTD local refinement 手法: EM (単粒子) / 解像度: 4.8 Å
化合物	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN*: Unknown entry
由来	saccharomyces cerevisiae (パン酵母) homo sapiens (ヒト)
キーワード	REPLICATION / helicase / polymerase / pol alpha / priming / replisome / human