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- EMDB-15924: S. cerevisiae pol alpha - replisome complex -

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Basic information

Entry
Database: EMDB / ID: EMD-15924
TitleS. cerevisiae pol alpha - replisome complex
Map dataPol alpha - replisome complex. Locally filtered
Sample
  • Complex: S. cerevisiae replisome - pol alpha primase complex
    • Protein or peptide: x 18 types
    • DNA: x 2 types
  • Ligand: x 3 types
KeywordsReplication / helicase / polymerase / pol alpha / priming / replisome
Function / homology
Function and homology information


Inhibition of replication initiation of damaged DNA by RB1/E2F1 / H3-H4 histone complex chaperone activity / maintenance of DNA repeat elements / Unwinding of DNA / replication fork arrest / regulation of nuclear cell cycle DNA replication / DNA replication initiation / meiotic chromosome segregation / RNA-templated DNA biosynthetic process / MCM core complex ...Inhibition of replication initiation of damaged DNA by RB1/E2F1 / H3-H4 histone complex chaperone activity / maintenance of DNA repeat elements / Unwinding of DNA / replication fork arrest / regulation of nuclear cell cycle DNA replication / DNA replication initiation / meiotic chromosome segregation / RNA-templated DNA biosynthetic process / MCM core complex / Assembly of the pre-replicative complex / Switching of origins to a post-replicative state / DNA strand elongation involved in mitotic DNA replication / MCM complex binding / Processive synthesis on the lagging strand / GINS complex / nuclear DNA replication / mitotic DNA replication preinitiation complex assembly / Removal of the Flap Intermediate / premeiotic DNA replication / Polymerase switching / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / anaphase-promoting complex binding / mitotic DNA replication / alpha DNA polymerase:primase complex / Activation of the pre-replicative complex / CMG complex / nuclear pre-replicative complex / DNA replication checkpoint signaling / DNA primase activity / establishment of mitotic sister chromatid cohesion / Activation of ATR in response to replication stress / DNA replication preinitiation complex / MCM complex / primosome complex / telomere capping / DNA replication, synthesis of primer / mitotic DNA replication checkpoint signaling / lagging strand elongation / replication fork protection complex / double-strand break repair via break-induced replication / cellular response to osmotic stress / mitotic DNA replication initiation / single-stranded DNA helicase activity / mitotic intra-S DNA damage checkpoint signaling / silent mating-type cassette heterochromatin formation / regulation of DNA-templated DNA replication initiation / DNA strand elongation involved in DNA replication / mitotic sister chromatid cohesion / DNA synthesis involved in DNA repair / DNA biosynthetic process / leading strand elongation / DNA unwinding involved in DNA replication / replication fork processing / nuclear replication fork / mitotic G2 DNA damage checkpoint signaling / DNA replication origin binding / DNA replication initiation / subtelomeric heterochromatin formation / Ub-specific processing proteases / DNA helicase activity / telomere maintenance / nuclear periphery / replication fork / meiotic cell cycle / helicase activity / transcription elongation by RNA polymerase II / heterochromatin formation / DNA-templated DNA replication / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / double-strand break repair / nuclear envelope / single-stranded DNA binding / 4 iron, 4 sulfur cluster binding / DNA helicase / DNA replication / DNA-directed DNA polymerase / chromosome, telomeric region / DNA-directed DNA polymerase activity / protein stabilization / DNA repair / nucleotide binding / DNA damage response / chromatin binding / ATP hydrolysis activity / mitochondrion / DNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
DNA replication checkpoint mediator, MRC1 domain / MRC1-like domain / Claspin / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / : / DNA polymerase alpha subunit B, OB domain / Timeless, N-terminal / Timeless ...DNA replication checkpoint mediator, MRC1 domain / MRC1-like domain / Claspin / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / : / DNA polymerase alpha subunit B, OB domain / Timeless, N-terminal / Timeless / Timeless protein / DNA polymerase alpha, subunit B / DNA primase, small subunit, eukaryotic/archaeal / DNA primase, large subunit, eukaryotic / DNA primase, small subunit / DNA primase small subunit / DNA primase large subunit, eukaryotic/archaeal / DNA polymerase alpha catalytic subunit, N-terminal domain / DNA polymerase alpha, zinc finger domain superfamily / Eukaryotic and archaeal DNA primase, large subunit / DNA Polymerase alpha zinc finger / DNA polymerase alpha subunit p180 N terminal / Zinc finger, DNA-directed DNA polymerase, family B, alpha / DNA polymerase alpha catalytic subunit, catalytic domain / : / MCM3 winged helix domain / GINS/PriA/YqbF domain / CDC45 family / DNA replication complex GINS protein Psf2 / CDC45-like protein / GINS complex, subunit Psf1 / GINS complex, subunit Psf3 / GINS complex, subunit Psf3 superfamily / DNA replication complex GINS protein SLD5, C-terminal / GINS, helical bundle-like domain superfamily / GINS complex protein Sld5, alpha-helical domain / DNA replication complex GINS protein SLD5 C-terminus / GINS complex subunit Sld5 / GINS subunit, domain A / GINS complex protein helical bundle domain / MCM4, winged helix domain / DNA polymerase alpha/delta/epsilon, subunit B / DNA polymerase alpha/epsilon subunit B / : / MCM5, C-terminal domain / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm6 / DNA replication licensing factor Mcm7 / Mcm6, C-terminal winged-helix domain / MCM6 C-terminal winged-helix domain / DNA replication licensing factor Mcm2 / Mini-chromosome maintenance protein 2 / Mini-chromosome maintenance, conserved site / MCM family signature. / MCM N-terminal domain / MCM N-terminal domain / MCM OB domain / MCM OB domain / Mini-chromosome maintenance protein / MCM, AAA-lid domain / MCM P-loop domain / MCM AAA-lid domain / MCM family domain profile. / minichromosome maintenance proteins / MCM domain / DNA polymerase family B, thumb domain / DNA-directed DNA polymerase, family B, multifunctional domain / DNA polymerase family B signature. / DNA-directed DNA polymerase, family B, conserved site / DNA polymerase family B / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA polymerase, palm domain superfamily / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Winged helix-like DNA-binding domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DNA primase small subunit / DNA polymerase alpha catalytic subunit A / DNA primase large subunit / DNA replication licensing factor MCM3 / Mediator of replication checkpoint protein 1 / DNA replication licensing factor MCM2 / Minichromosome maintenance protein 5 / DNA replication licensing factor MCM4 / DNA polymerase alpha subunit B / DNA replication licensing factor MCM7 ...DNA primase small subunit / DNA polymerase alpha catalytic subunit A / DNA primase large subunit / DNA replication licensing factor MCM3 / Mediator of replication checkpoint protein 1 / DNA replication licensing factor MCM2 / Minichromosome maintenance protein 5 / DNA replication licensing factor MCM4 / DNA polymerase alpha subunit B / DNA replication licensing factor MCM7 / DNA replication complex GINS protein PSF2 / DNA replication licensing factor MCM6 / Topoisomerase 1-associated factor 1 / DNA replication complex GINS protein SLD5 / Chromosome segregation in meiosis protein 3 / Cell division control protein 45 / DNA replication complex GINS protein PSF3 / DNA replication complex GINS protein PSF1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.6 Å
AuthorsJones ML / Yeeles JTP
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_1201/12 United Kingdom
CitationJournal: Mol Cell / Year: 2023
Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication.
Authors: Morgan L Jones / Valentina Aria / Yasemin Baris / Joseph T P Yeeles /
Abstract: During eukaryotic DNA replication, Pol α-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides during discontinuous lagging-strand ...During eukaryotic DNA replication, Pol α-primase generates primers at replication origins to start leading-strand synthesis and every few hundred nucleotides during discontinuous lagging-strand replication. How Pol α-primase is targeted to replication forks to prime DNA synthesis is not fully understood. Here, by determining cryoelectron microscopy (cryo-EM) structures of budding yeast and human replisomes containing Pol α-primase, we reveal a conserved mechanism for the coordination of priming by the replisome. Pol α-primase binds directly to the leading edge of the CMG (CDC45-MCM-GINS) replicative helicase via a complex interaction network. The non-catalytic PRIM2/Pri2 subunit forms two interfaces with CMG that are critical for in vitro DNA replication and yeast cell growth. These interactions position the primase catalytic subunit PRIM1/Pri1 directly above the exit channel for lagging-strand template single-stranded DNA (ssDNA), revealing why priming occurs efficiently only on the lagging-strand template and elucidating a mechanism for Pol α-primase to overcome competition from RPA to initiate primer synthesis.
History
DepositionOct 5, 2022-
Header (metadata) releaseAug 9, 2023-
Map releaseAug 9, 2023-
UpdateAug 30, 2023-
Current statusAug 30, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15924.png

Downloads & links

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Map

FileDownload / File: emd_15924.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPol alpha - replisome complex. Locally filtered
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
2.27 Å/pix.
x 220 pix.
= 498.806 Å		2.27 Å/pix.
x 220 pix.
= 498.806 Å		2.27 Å/pix.
x 220 pix.
= 498.806 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 2.2673 Å
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Contour LevelBy AUTHOR: 1.68
Minimum - Maximum-6.1066723 - 11.148357000000001
Average (Standard dev.)0.035365876 (±0.30480275)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 498.80597 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #2

Fileemd_15924_additional_1.map
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Additional map: #1

Fileemd_15924_additional_2.map
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Half map: Half map A

Fileemd_15924_half_map_1.map
AnnotationHalf map A
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Half map: Half map B

Fileemd_15924_half_map_2.map
AnnotationHalf map B
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Sample components

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Entire : S. cerevisiae replisome - pol alpha primase complex

EntireName: S. cerevisiae replisome - pol alpha primase complex
Components
  • Complex: S. cerevisiae replisome - pol alpha primase complex
    • Protein or peptide: DNA replication licensing factor MCM2
    • Protein or peptide: DNA replication licensing factor MCM3
    • Protein or peptide: DNA replication licensing factor MCM4
    • Protein or peptide: Minichromosome maintenance protein 5
    • Protein or peptide: DNA replication licensing factor MCM6
    • Protein or peptide: DNA replication licensing factor MCM7
    • Protein or peptide: DNA primase large subunit
    • Protein or peptide: DNA polymerase alpha subunit B
    • Protein or peptide: DNA replication complex GINS protein PSF1
    • Protein or peptide: DNA replication complex GINS protein PSF2
    • Protein or peptide: DNA replication complex GINS protein PSF3
    • Protein or peptide: DNA replication complex GINS protein SLD5
    • Protein or peptide: Cell division control protein 45
    • Protein or peptide: Mediator of replication checkpoint protein 1
    • DNA: Leading strand
    • DNA: Lagging strand
    • Protein or peptide: DNA primase small subunit
    • Protein or peptide: Topoisomerase 1-associated factor 1
    • Protein or peptide: Chromosome segregation in meiosis protein 3
    • Protein or peptide: DNA polymerase alpha catalytic subunit A
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: S. cerevisiae replisome - pol alpha primase complex

SupramoleculeName: S. cerevisiae replisome - pol alpha primase complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#20
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: DNA replication licensing factor MCM2

MacromoleculeName: DNA replication licensing factor MCM2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 98.911539 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD IDEVEEQMNE VDLMDDNMYE DYAADHNRD RYDPDQVDDR EQQELSLSER RRIDAQLNER DRLLRNVAYI DDEDEEQEGA AQLDEMGLPV QRRRRRRQYE D LENSDDDL ...String:
MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD IDEVEEQMNE VDLMDDNMYE DYAADHNRD RYDPDQVDDR EQQELSLSER RRIDAQLNER DRLLRNVAYI DDEDEEQEGA AQLDEMGLPV QRRRRRRQYE D LENSDDDL LSDMDIDPLR EELTLESLSN VKANSYSEWI TQPNVSRTIA RELKSFLLEY TDETGRSVYG ARIRTLGEMN SE SLEVNYR HLAESKAILA LFLAKCPEEM LKIFDLVAME ATELHYPDYA RIHSEIHVRI SDFPTIYSLR ELRESNLSSL VRV TGVVTR RTGVFPQLKY VKFNCLKCGS ILGPFFQDSN EEIRISFCTN CKSKGPFRVN GEKTVYRNYQ RVTLQEAPGT VPPG RLPRH REVILLADLV DVSKPGEEVE VTGIYKNNYD GNLNAKNGFP VFATIIEANS IKRREGNTAN EGEEGLDVFS WTEEE EREF RKISRDRGII DKIISSMAPS IYGHRDIKTA VACSLFGGVP KNVNGKHSIR GDINVLLLGD PGTAKSQILK YVEKTA HRA VFATGQGASA VGLTASVRKD PITKEWTLEG GALVLADKGV CLIDEFDKMN DQDRTSIHEA MEQQSISISK AGIVTTL QA RCSIIAAANP NGGRYNSTLP LAQNVSLTEP ILSRFDILCV VRDLVDEEAD ERLATFVVDS HVRSHPENDE DREGEELK N NGESAIEQGE DEINEQLNAR QRRLQRQRKK EEEISPIPQE LLMKYIHYAR TKIYPKLHQM DMDKVSRVYA DLRRESIST GSFPITVRHL ESILRIAESF AKMRLSEFVS SYDLDRAIKV VVDSFVDAQK VSVRRQLRRS FAIYTLGH

UniProtKB: DNA replication licensing factor MCM2

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Macromolecule #2: DNA replication licensing factor MCM3

MacromoleculeName: DNA replication licensing factor MCM3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 111.987562 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEAPVME GSTGFDGDAT TFFAPDAVFG DRVRRFQEFL DTFTSYRDSV RSIQVYNSN NAANYNDDQD DADERDLLGD DDGDDLEKEK KAASSTSLNI LPHRIIISLD DLREFDRSFW SGILVEPAYF I PPAEKALT ...String:
MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEAPVME GSTGFDGDAT TFFAPDAVFG DRVRRFQEFL DTFTSYRDSV RSIQVYNSN NAANYNDDQD DADERDLLGD DDGDDLEKEK KAASSTSLNI LPHRIIISLD DLREFDRSFW SGILVEPAYF I PPAEKALT DLADSMDDVP HPNASAVSSR HPWKLSFKGS FGAHALSPRT LTAQHLNKLV SVEGIVTKTS LVRPKLIRSV HY AAKTGRF HYRDYTDATT TLTTRIPTPA IYPTEDTEGN KLTTEYGYST FIDHQRITVQ EMPEMAPAGQ LPRSIDVILD DDL VDKTKP GDRVNVVGVF KSLGAGGMNQ SNSNTLIGFK TLILGNTVYP LHARSTGVAA RQMLTDFDIR NINKLSKKKD IFDI LSQSL APSIYGHDHI KKAILLMLMG GVEKNLENGS HLRGDINILM VGDPSTAKSQ LLRFVLNTAS LAIATTGRGS SGVGL TAAV TTDRETGERR LEAGAMVLAD RGVVCIDEFD KMTDVDRVAI HEVMEQQTVT IAKAGIHTTL NARCSVIAAA NPVFGQ YDV NRDPHQNIAL PDSLLSRFDL LFVVTDDINE IRDRSISEHV LRTHRYLPPG YLEGEPVRER LNLSLAVGED ADINPEE HS NSGAGVENEG EDDEDHVFEK FNPLLQAGAK LAKNKGNYNG TEIPKLVTIP FLRKYVQYAK ERVIPQLTQE AINVIVKN Y TDLRNDDNTK KSPITARTLE TLIRLATAHA KVRLSKTVNK VDAKVAANLL RFALLGEDIG NDIDEEESEY EEALSKRSP QKSPKKRQRV RQPASNSGSP IKSTPRRSTA SSVNATPSSA RRILRFQDDE QNAGEDDNDI MSPLPADEEA ELQRRLQLGL RVSPRRREH LHAPEEGSSG PLTEVGTPRL PNVSSAGQDD EQQQSVISFD NVEPGTISTG RLSLISGIIA RLMQTEIFEE E SYPVASLF ERINEELPEE EKFSAQEYLA GLKIMSDRNN LMVADDKVWR V

UniProtKB: DNA replication licensing factor MCM3

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Macromolecule #3: DNA replication licensing factor MCM4

MacromoleculeName: DNA replication licensing factor MCM4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 105.138375 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQG RQGRIRSSAS ASGRSRYHSD LRSDRALPTS SSSLGRNGQN RVHMRRNDIH TSDLSSPRRI VDFDTRSGVN T LDTSSSSA ...String:
MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQG RQGRIRSSAS ASGRSRYHSD LRSDRALPTS SSSLGRNGQN RVHMRRNDIH TSDLSSPRRI VDFDTRSGVN T LDTSSSSA PPSEASEPLR IIWGTNVSIQ ECTTNFRNFL MSFKYKFRKI LDEREEFINN TTDEELYYIK QLNEMRELGT SN LNLDARN LLAYKQTEDL YHQLLNYPQE VISIMDQTIK DCMVSLIVDN NLDYDLDEIE TKFYKVRPYN VGSCKGMREL NPN DIDKLI NLKGLVLRST PVIPDMKVAF FKCNVCDHTM AVEIDRGVIQ EPARCERIDC NEPNSMSLIH NRCSFADKQV IKLQ ETPDF VPDGQTPHSI SLCVYDELVD SCRAGDRIEV TGTFRSIPIR ANSRQRVLKS LYKTYVDVVH VKKVSDKRLD VDTST IEQE LMQNKVDHNE VEEVRQITDQ DLAKIREVAA REDLYSLLAR SIAPSIYELE DVKKGILLQL FGGTNKTFTK GGRYRG DIN ILLCGDPSTS KSQILQYVHK ITPRGVYTSG KGSSAVGLTA YITRDVDTKQ LVLESGALVL SDGGVCCIDE FDKMSDS TR SVLHEVMEQQ TISIAKAGII TTLNARSSIL ASANPIGSRY NPNLPVTENI DLPPPLLSRF DLVYLVLDKV DEKNDREL A KHLTNLYLED KPEHISQDDV LPVEFLTMYI SYAKEHIHPI ITEAAKTELV RAYVGMRKMG DDSRSDEKRI TATTRQLES MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP KTGKIDMNLV QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQ INEHSQDRVE SSDIQEALSR LQQEDKVIVL GEGVRRSVRL NNRV

UniProtKB: DNA replication licensing factor MCM4

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Macromolecule #4: Minichromosome maintenance protein 5

MacromoleculeName: Minichromosome maintenance protein 5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 86.505734 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPL FETAITQVAK RISILSRAQS ANNNDKDPEN TSMDTDSLLL NSLPTFQLIL NSNANQIPLR DLDSEHVSKI V RLSGIIIS ...String:
MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPL FETAITQVAK RISILSRAQS ANNNDKDPEN TSMDTDSLLL NSLPTFQLIL NSNANQIPLR DLDSEHVSKI V RLSGIIIS TSVLSSRATY LSIMCRNCRH TTSITINNFN SITGNTVSLP RSCLSTIESE SSMANESNIG DESTKKNCGP DP YIIIHES SKFIDQQFLK LQEIPELVPV GEMPRNLTMT CDRYLTNKVI PGTRVTIVGI YSIYNSKNGA GSGRSGGGNG GSG VAIRTP YIKILGIQSD VETSSIWNSV TMFTEEEEEE FLQLSRNPKL YEILTNSIAP SIFGNEDIKK AIVCLLMGGS KKIL PDGMR LRGDINVLLL GDPGTAKSQL LKFVEKVSPI AVYTSGKGSS AAGLTASVQR DPMTREFYLE GGAMVLADGG VVCID EFDK MRDEDRVAIH EAMEQQTISI AKAGITTVLN SRTSVLAAAN PIYGRYDDLK SPGDNIDFQT TILSRFDMIF IVKDDH NEE RDISIANHVI NIHTGNANAM QNQQEENGSE ISIEKMKRYI TYCRLKCAPR LSPQAAEKLS SNFVTIRKQL LINELES TE RSSIPITIRQ LEAIIRITES LAKLELSPIA QERHVDEAIR LFQASTMDAA SQDPIGGLNQ ASGTSLSEIR RFEQELKR R LPIGWSTSYQ TLRREFVDTH RFSQLALDKA LYALEKHETI QLRHQGQNIY RSGV

UniProtKB: Minichromosome maintenance protein 5

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Macromolecule #5: DNA replication licensing factor MCM6

MacromoleculeName: DNA replication licensing factor MCM6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 113.110211 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP FVNDSTQFSS QRLQTDGSAT NDMEGNEPA RSFKSRALNH VKKVDDVTGE KVREAFEQFL EDFSVQSTDT GEVEKVYRAQ IEFMKIYDLN TIYIDYQHLS M RENGALAM ...String:
MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP FVNDSTQFSS QRLQTDGSAT NDMEGNEPA RSFKSRALNH VKKVDDVTGE KVREAFEQFL EDFSVQSTDT GEVEKVYRAQ IEFMKIYDLN TIYIDYQHLS M RENGALAM AISEQYYRFL PFLQKGLRRV VRKYAPELLN TSDSLKRSEG DEGQADEDEQ QDDDMNGSSL PRDSGSSAAP GN GTSAMAT RSITTSTSPE QTERVFQISF FNLPTVHRIR DIRSEKIGSL LSISGTVTRT SEVRPELYKA SFTCDMCRAI VDN VEQSFK YTEPTFCPNP SCENRAFWTL NVTRSRFLDW QKVRIQENAN EIPTGSMPRT LDVILRGDSV ERAKPGDRCK FTGV EIVVP DVTQLGLPGV KPSSTLDTRG ISKTTEGLNS GVTGLRSLGV RDLTYKISFL ACHVISIGSN IGASSPDANS NNRET ELQM AANLQANNVY QDNERDQEVF LNSLSSDEIN ELKEMVKDEH IYDKLVRSIA PAVFGHEAVK KGILLQMLGG VHKSTV EGI KLRGDINICV VGDPSTSKSQ FLKYVVGFAP RSVYTSGKAS SAAGLTAAVV RDEEGGDYTI EAGALMLADN GICCIDE FD KMDISDQVAI HEAMEQQTIS IAKAGIHATL NARTSILAAA NPVGGRYNRK LSLRGNLNMT APIMSRFDLF FVILDDCN E KIDTELASHI VDLHMKRDEA IEPPFSAEQL RRYIKYARTF KPILTKEARS YLVEKYKELR KDDAQGFSRS SYRITVRQL ESMIRLSEAI ARANCVDEIT PSFIAEAYDL LRQSIIRVDV DDVEMDEEFD NIESQSHAAS GNNDDNDDGT GSGVITSEPP ADIEEGQSE ATARPGTSEK KKTTVTYDKY VSMMNMIVRK IAEVDREGAE ELTAVDIVDW YLLQKENDLG SLAEYWEERR L AFKVIKRL VKDRILMEIH GTRHNLRDLE NEENENNKTV YVIHPNCEVL DQLEPQDSS

UniProtKB: DNA replication licensing factor MCM6

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Macromolecule #6: DNA replication licensing factor MCM7

MacromoleculeName: DNA replication licensing factor MCM7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 95.049875 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG PKYMAMLQKV ANRELNSVII DLDDILQYQ NEKFLQGTQA DDLVSAIQQN ANHFTELFCR AIDNNMPLPT KEIDYKDDVL DVILNQRRLR NERMLSDRTN E IRSENLMD ...String:
MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG PKYMAMLQKV ANRELNSVII DLDDILQYQ NEKFLQGTQA DDLVSAIQQN ANHFTELFCR AIDNNMPLPT KEIDYKDDVL DVILNQRRLR NERMLSDRTN E IRSENLMD TTMDPPSSMN DALREVVEDE TELFPPNLTR RYFLYFKPLS QNCARRYRKK AISSKPLSVR QIKGDFLGQL IT VRGIITR VSDVKPAVEV IAYTCDQCGY EVFQEVNSRT FTPLSECTSE ECSQNQTKGQ LFMSTRASKF SAFQECKIQE LSQ QVPVGH IPRSLNIHVN GTLVRSLSPG DIVDVTGIFL PAPYTGFKAL KAGLLTETYL EAQFVRQHKK KFASFSLTSD VEER VMELI TSGDVYNRLA KSIAPEIYGN LDVKKALLLL LVGGVDKRVG DGMKIRGDIN VCLMGDPGVA KSQLLKAICK ISPRG VYTT GKGSSGVGLT AAVMKDPVTD EMILEGGALV LADNGICCID EFDKMDESDR TAIHEVMEQQ TISISKAGIN TTLNAR TSI LAAANPLYGR YNPRLSPLDN INLPAALLSR FDILFLMLDI PSRDDDEKLA EHVTYVHMHN KQPDLDFTPV EPSKMRE YI AYAKTKRPVM SEAVNDYVVQ AYIRLRQDSK REMDSKFSFG QATPRTLLGI IRLSQALAKL RLADMVDIDD VEEALRLV R VSKESLYQET NKSKEDESPT TKIFTIIKKM LQETGKNTLS YENIVKTVRL RGFTMLQLSN CIQEYSYLNV WHLINEGNT LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI DLQDA

UniProtKB: DNA replication licensing factor MCM7

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Macromolecule #7: DNA primase large subunit

MacromoleculeName: DNA primase large subunit / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 62.348551 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL YARLYESKLS FYDLPPQGEI TLEQFEIWAI DRLKILLEI ESCLSRNKSI KEIETIIKPQ FQKLLPFNTE SLEDRKKDYY SHFILRLCFC RSKELREKFV RAETFLFKIR F NMLTSTDQ ...String:
MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL YARLYESKLS FYDLPPQGEI TLEQFEIWAI DRLKILLEI ESCLSRNKSI KEIETIIKPQ FQKLLPFNTE SLEDRKKDYY SHFILRLCFC RSKELREKFV RAETFLFKIR F NMLTSTDQ TKFVQSLDLP LLQFISNEEK AELSHQLYQT VSASLQFQLN LNEEHQRKQY FQQEKFIKLP FENVIELVGN RL VFLKDGY AYLPQFQQLN LLSNEFASKL NQELIKTYQY LPRLNEDDRL LPILNHLSSG YTIADFNQQK ANQFSENVDD EIN AQSVWS EEISSNYPLC IKNLMEGLKK NHHLRYYGRQ QLSLFLKGIG LSADEALKFW SEAFTRNGNM TMEKFNKEYR YSFR HNYGL EGNRINYKPW DCHTILSKPR PGRGDYHGCP FRDWSHERLS AELRSMKLTQ AQIISVLDSC QKGEYTIACT KVFEM THNS ASADLEIGEQ THIAHPNLYF ERSRQLQKKQ QKLEKEKLFN NGNH

UniProtKB: DNA primase large subunit

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Macromolecule #8: DNA polymerase alpha subunit B

MacromoleculeName: DNA polymerase alpha subunit B / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 78.865938 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR QTHTDLTSKN IDEFKQFLQL QMEKRANQIS SSSKVNTST KKPVIKKSLN SSPLFGLSIP KTPTLKKRKL HGPFSLSDSK QTYNVGSEAE TNEKGNSSLK LEFTPGMAED A VGDSAPLS ...String:
MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR QTHTDLTSKN IDEFKQFLQL QMEKRANQIS SSSKVNTST KKPVIKKSLN SSPLFGLSIP KTPTLKKRKL HGPFSLSDSK QTYNVGSEAE TNEKGNSSLK LEFTPGMAED A VGDSAPLS HAKSSDAKTP GSSTFQTPTT NTPTTSRQNV PAGEILDSLN PENIEISSGN PNVGLLSTEE PSYNQVKVEP FY DAKKYKF RTMRQNLQEA SDVLDDQIES FTKIIQNHYK LSPNDFADPT IQSQSEIYAV GRIVPDSPTY DKFLNPESLS LET SRMGGV GRRVRLDLSQ VNELSFFLGQ IVAFKGKNAN GDYFTVNSIL PLPYPNSPVS TSQELQEFQA NLEGSSLKVI VTCG PYFAN DNFSLELLQE FIDSINNEVK PHVLIMFGPF IDITHPLIAS GKLPNFPQFK TQPKTLDELF LKLFTPILKT ISPHI QTVL IPSTKDAISN HAAYPQASLI RKALQLPKRN FKCMANPSSF QINEIYFGCS NVDTFKDLKE VIKGGTTSSR YRLDRV SEH ILQQRRYYPI FPGSIRTRIK PKDVSTKKET NDMESKEEKV YEHISGADLD VSYLGLTEFV GGFSPDIMII PSELQHF AR VVQNVVVINP GRFIRATGNR GSYAQITVQC PDLEDGKLTL VEGEEPVYLH NVWKRARVDL IAS

UniProtKB: DNA polymerase alpha subunit B

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Macromolecule #9: DNA replication complex GINS protein PSF1

MacromoleculeName: DNA replication complex GINS protein PSF1 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.230576 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAY QRLRTDILDS MAWNNNGLDL MSSITFSQQD TNNLSHQEQE YLKEYCDLIT DLKSGDLVDI DLSGSLVPPS D VFIDVRVL ...String:
MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAY QRLRTDILDS MAWNNNGLDL MSSITFSQQD TNNLSHQEQE YLKEYCDLIT DLKSGDLVDI DLSGSLVPPS D VFIDVRVL KDAGEIQTEY GVFNLIKDSQ FFVRQSDVER LIQQGYLQKI

UniProtKB: DNA replication complex GINS protein PSF1

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Macromolecule #10: DNA replication complex GINS protein PSF2

MacromoleculeName: DNA replication complex GINS protein PSF2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 25.096807 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL NNMVAMRSTE VVLWIALLLK QQSKCSIVA PQWLTTKELD RKIQYEKTHP DRFSELPWNW LVLARILFNK AKDDFHDPIH ELRGKIQDLR EIRQIKVLKG L KYLNESHL ...String:
MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL NNMVAMRSTE VVLWIALLLK QQSKCSIVA PQWLTTKELD RKIQYEKTHP DRFSELPWNW LVLARILFNK AKDDFHDPIH ELRGKIQDLR EIRQIKVLKG L KYLNESHL QLDNLSLLEI NELRPFITEI MDKLREIHTA SLTAGTENDE EEFNI

UniProtKB: DNA replication complex GINS protein PSF2

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Macromolecule #11: DNA replication complex GINS protein PSF3

MacromoleculeName: DNA replication complex GINS protein PSF3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.437859 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MGSSHHHHHH SSGLVPRGSH MASMGYYDID DVLADGTEFP CKFQYDIPGL GYLENNPGRP ITKNTKLSLP LWLARILAIV GGDEALVDE EPVPFVELLP PDMFSTKVMN AIKTDPVALD LHSINSHFFS LAIKWIMLFS EKELANVVSE LLLQRAQELN H HASSLSID ...String:
MGSSHHHHHH SSGLVPRGSH MASMGYYDID DVLADGTEFP CKFQYDIPGL GYLENNPGRP ITKNTKLSLP LWLARILAIV GGDEALVDE EPVPFVELLP PDMFSTKVMN AIKTDPVALD LHSINSHFFS LAIKWIMLFS EKELANVVSE LLLQRAQELN H HASSLSID LNADSTGKNS ANTNIATSTF LLKLEEMEKE IYKKSHESYK DTKRWMFKK

UniProtKB: DNA replication complex GINS protein PSF3

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Macromolecule #12: DNA replication complex GINS protein SLD5

MacromoleculeName: DNA replication complex GINS protein SLD5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 33.983617 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLN RISMQSQLIE NISMGFLDMQ NASNANPPMP NESKLPLLCM ETELERLKFV IRSYIRCRLS KIDKFSLYLR Q LNEDENSL ...String:
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLN RISMQSQLIE NISMGFLDMQ NASNANPPMP NESKLPLLCM ETELERLKFV IRSYIRCRLS KIDKFSLYLR Q LNEDENSL ISLTDLLSKD EIKYHDTHSL IWLKLVNDSI LKYMPEELQA INDTEGSVNM IDEPDWNKFV FIHVNGPPDG KW NEDPLLQ ENEFGKPCYT VTIPDLKEEV ELTIGSIYVM RYEVIRDLLR DDKVALI

UniProtKB: DNA replication complex GINS protein SLD5

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Macromolecule #13: Cell division control protein 45

MacromoleculeName: Cell division control protein 45 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 75.154703 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGV IDLEAFLEID PQEYVIDTDE KSGEQSFRRD IYVLDAHRPW NLDNIFGSQI IQCFDDGTVD DTLGEQKEAY Y KLLELDEE ...String:
MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGV IDLEAFLEID PQEYVIDTDE KSGEQSFRRD IYVLDAHRPW NLDNIFGSQI IQCFDDGTVD DTLGEQKEAY Y KLLELDEE SGDDELSGDE NDNNGGDDEA TDADEVTDED YKDDDGDYKD DDETISNKRG NSSIGPNDLS KRKQRKKQIH EY EGVLEEY YSQGTTVVNS ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK RLTPSSRNSV KTP DTLTLN IQPDYYLFLL RHSSLYDSFY YSNYVNAKLS LWNENGKKRL HKMFARMGIP LSTAQETWLY MDHSIKRELG IIFD KNLDR YGLQDIIRDG FVRTLGYRGS ISASEFVEAL TALLEVGNST DKDSVKINND NNDDTDGEEE EDNSAQKLTN LRKRW VSNF WLSWDALDDR KVELLNRGIQ LAQDLQRAIF NTGVAILEKK LIKHLRIYRL CVLQDGPDLD LYRNPLTLLR LGNWLI ECC AESEDKQLLP MVLASIDENT DTYLVAGLTP RYPRGLDTIH TKKPILNNFS MAFQQITAET DAKVRIDNFE SSIIEIR RE DLSPFLEKLT LSGLL

UniProtKB: Cell division control protein 45, Cell division control protein 45

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Macromolecule #14: Mediator of replication checkpoint protein 1

MacromoleculeName: Mediator of replication checkpoint protein 1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 126.078859 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MDDALHALSS LTAKKRTTTY KKVAVPILDE NDNTNGNGPN DIDNPPELTG NGFLFANATL NRVKNRLEGK KAPEQNHNNG KDRSENSLP TQLISNLYDG GEELEKSEVK DNSYSEKNVS SSFTQTQRIP VSIQQDKVFN VPIHSVNDGK PTQLIKEDGL V NETSQALK ...String:
MDDALHALSS LTAKKRTTTY KKVAVPILDE NDNTNGNGPN DIDNPPELTG NGFLFANATL NRVKNRLEGK KAPEQNHNNG KDRSENSLP TQLISNLYDG GEELEKSEVK DNSYSEKNVS SSFTQTQRIP VSIQQDKVFN VPIHSVNDGK PTQLIKEDGL V NETSQALK TPLTTGRPGA TQRIDSSGAT SQTQPIKSIE PQSQIITTSS NHSNALSPKI PIIPTELIGT SPLFQSIQNR GP DTQMDVP PQTAHDEDKT QAIGIPQATH QEQKTQIDTV AQTLQDEVPH TLKIREIQSE LASEDSKREK ARNVEYKKPQ KPI PTKKFF SKESFLADFD DSSSNEDDDI KLENAHPKPV QNDDELHENK SVELNLTDET RINEKRVPLL SSYANNLKRE IDSS KCITL DLDSDSDEYG DDDMDSIKLS KDESVLPISQ LSKATILNLK ARLSKQNQKL SQRPNKSKDP KVDHNVLLNT LRKAS RKQI LDHQKEVIET KGLKLEDMAK EKEIVENLLE QEILRNKRIR QKEKRREKLE ENDFQLNAHD SGSDSGSESS GFALSG NEI ADYESSGSEN DNRRESDSEK EDDEIILKQK KSHHVKHIIN ESDSDTEVEA KPKEKADESL PKRIAINLGH YGDNIGE DT DKFQETNVLD TQNIEEVMAE RNTIENEVKD DVYVNEEADE AIRRQLIDKE KLQLKQKEKE HEAKIKELKK RGVTNFFE M EAEESEDEWH GIGGADGEGS DDYDSDLEKM IDDYSKNNFN PHEIREMLAA ENKEMDIKMI NKILYDIKNG GFRNKRAKN SLELELSDDD EDDVLQQYRL KRRELMRKRR LEIGDDAKLV KNPKSSAFFE SMVEDIIEYK NPFGAEEEYN LDITSTATDL DTQDNSINV GDNTGNNEQK PVDQKNKKVI ISEDFVQKSL SFLKSNNYED FETDKELSRI QHGNDEAIED LYTLKQNSSI K SFTNSQTD STTSKTVNTI IDLEKRPEDE DEVENGDTSL VGVFKHPSII KSFASRTDIN DKFKEGNKTV KILKSYKTVG SS KASITYM GKTRKLIAPK RKTEGSHRYH HDHHNKKMKM KTKTKSNKLF ESGQDSFDND YKDDDGDYKD DD

UniProtKB: Mediator of replication checkpoint protein 1

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Macromolecule #17: DNA primase small subunit

MacromoleculeName: DNA primase small subunit / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 51.82709 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEMTNSV KTNGPSSSDM EYYYKSLYPF KHIFNWLNHS PKPSRDMINR EFAMAFRSG AYKRYNSFNS VQDFKAQIEK ANPDRFEIGA IYNKPPRERD TLLKSELKAL EKELVFDIDM DDYDAFRTCC S GAQVCSKC ...String:
MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEMTNSV KTNGPSSSDM EYYYKSLYPF KHIFNWLNHS PKPSRDMINR EFAMAFRSG AYKRYNSFNS VQDFKAQIEK ANPDRFEIGA IYNKPPRERD TLLKSELKAL EKELVFDIDM DDYDAFRTCC S GAQVCSKC WKFISLAMKI TNTALREDFG YKDFIWVFSG RRGAHCWVSD KRARALTDVQ RRNVLDYVNV IRDRNTDKRL AL KRPYHPH LARSLEQLKP FFVSIMLEEQ NPWEDDQHAI QTLLPALYDK QLIDSLKKYW LDNPRRSSKE KWNDIDQIAT SLF KGPKQD SHIIKLRECK EDLVLMTLYP KLDVEVTKQT IHLLKAPFCI HPATGNVCVP IDESFAPEKA PKLIDLQTEM EKNN DVSLT ALQPFINQFQ AYVSSLLKNE LGSVKRERED DDEPASLDF

UniProtKB: DNA primase small subunit

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Macromolecule #18: Topoisomerase 1-associated factor 1

MacromoleculeName: Topoisomerase 1-associated factor 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 141.296875 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLL PILIDWENKC SLAAKLAKNN PDHEEFRNKA YYDKIALNCL QLLVLMTWPL IVTEQSSSNQ ITLYGELKKH Q LVYKKTIL ...String:
MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLL PILIDWENKC SLAAKLAKNN PDHEEFRNKA YYDKIALNCL QLLVLMTWPL IVTEQSSSNQ ITLYGELKKH Q LVYKKTIL SMESGKVLRA AIRLALDVIK IDRLSRTPRD NMVLKLVLNF FRNVIAIEPG EFTINTKKSM PKKGITSIDT LP PNVSMDD ISLNTVISSF HKNKVFGFLL TLTSSLSKEF DQDFINIPLL EIMFYFTKDV NQELLFPRQF ETGTHSKVVN KNE SSSANN IVTSAGFELS KLLQKEHQMR KNVIKHTSAR HSRFGGLLSI QTPDKTRLTV SGSQALVDEK IALQKLDDSK KWNK RIIKK HQSVAAEGLP NSLLNSQTGK AIFFTESNGK HFKEFINNFI DSGFNILLHS VTNYFTTEQD RMVTLEQVEY LLFFA WFVK YQLLRSKIDN SADIKQVSEA LKEVTFILVS SLLRSAYDLK NWTVTHAGMI AFNELLNLVS RTKAAQEEDS TDIEFI VSR LFSDERIQLL SNLPKIGSKY SLQFMKSCIE LTHSVLKVLE QYSDDKTLVI EGKSRRQKKF NISEGDITKL IEEENVD RD EALDILTSSL RSIEVNFQKV QANYMTEPVI ETYINFLERF RELEDDSIKK VFSFFHRVFV QAKEQALLFR FDLIILLR E MLSPDGLDRM SRSRKYVSQF SDYFLARLKK RLKKSPAWFV GLLFPPLHNS EVGFYQRYGE YNVLNNESMY AAPASQFKP IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRAL LLLIGYSIPR KINEPCFLPG TVEVSDLTVS CELVKKYLST PFETPNGLPS SSYLLRVRSE KDSFSHNEQD G WEGDDDYD YNDPYIVPDD QILSKSDAAY FKDLDNNASD KLKGTKFSKG IARSKKKDKR KRRKGEAKTN LPMFGDQDDE RP QTVRERH GVFSKEFISD SEDDEDLMNP IFFENETYMR WLLDKNNGQL TEDRYIQFAK FAAERMNNGG VVTGDYTSLF GGS IPSIES IRATESSSFA PDKSLISLAS HVASEMSIFD VNNNNNNQLS DDDVNSESRN SLGSSQPSNS QNMFQSEVYS RKES TKRSL EASAADESDE DEEAIRLFGK KSRVVLSQGD SDD

UniProtKB: Topoisomerase 1-associated factor 1

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Macromolecule #19: Chromosome segregation in meiosis protein 3

MacromoleculeName: Chromosome segregation in meiosis protein 3 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 36.588758 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: GEMDQDFDSL LLGFNDSDSV QKDPTVPNGL DGSVVDPTIA DPTAITARKR RPQVKLTAEK LLSDKGLPYV LKNAHKRIRI SSKKNSYDN LSNIIQFYQL WAHELFPKAK FKDFMKICQT VGKTDPVLRE YRVSLFRDEM GMSFDVGTRE TGQDLERQSP M VEEHVTSA ...String:
GEMDQDFDSL LLGFNDSDSV QKDPTVPNGL DGSVVDPTIA DPTAITARKR RPQVKLTAEK LLSDKGLPYV LKNAHKRIRI SSKKNSYDN LSNIIQFYQL WAHELFPKAK FKDFMKICQT VGKTDPVLRE YRVSLFRDEM GMSFDVGTRE TGQDLERQSP M VEEHVTSA EERPIVADSF AQDKRNVNNV DYDNDEDDDI YHLSYRNRRG RVLDERGNNE TVLNNVVPPK EDLDALLKTF RV QGPVGLE ENEKKLLLGW LDAHRKMEKG SMTEEDVQLI QSLEEWEMND IEGQHTHYDL LPGGDEFGVD QDELDAMKEM GF

UniProtKB: Chromosome segregation in meiosis protein 3

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Macromolecule #20: DNA polymerase alpha catalytic subunit A

MacromoleculeName: DNA polymerase alpha catalytic subunit A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed DNA polymerase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 167.027766 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSSKSEKLEK LRKLQAARNG TSIDDYEGDE SDGDRIYDEI DEKEYRARKR QELLHDDFVV DDDGVGYVDR GVEEDWREVD NSSSDEDTG NLASKDSKRK KNIKREKDHQ ITDMLRTQHS KSTLLAHAKK SQKKSIPIDN FDDILGEFES GEVEKPNILL P SKLRENLN ...String:
MSSKSEKLEK LRKLQAARNG TSIDDYEGDE SDGDRIYDEI DEKEYRARKR QELLHDDFVV DDDGVGYVDR GVEEDWREVD NSSSDEDTG NLASKDSKRK KNIKREKDHQ ITDMLRTQHS KSTLLAHAKK SQKKSIPIDN FDDILGEFES GEVEKPNILL P SKLRENLN SSPTSEFKSS IKRVNGNDES SHDAGISKKV KIDPDSSTDK YLEIESSPLK LQSRKLRYAN DVQDLLDDVE NS PVVATKR QNVLQDTLLA NPPSAQSLAD EEDDEDSDED IILKRRTMRS VTTTRRVNID SRSNPSTSPF VTAPGTPIGI KGL TPSKSL QSNTDVATLA VNVKKEDVVD PETDTFQMFW LDYCEVNNTL ILFGKVKLKD DNCVSAMVQI NGLCRELFFL PREG KTPTD IHEEIIPLLM DKYGLDNIRA KPQKMKYSFE LPDIPSESDY LKVLLPYQTP KSSRDTIPSD LSSDTFYHVF GGNSN IFES FVIQNRIMGP CWLDIKGADF NSIRNASHCA VEVSVDKPQN ITPTTTKTMP NLRCLSLSIQ TLMNPKENKQ EIVSIT LSA YRNISLDSPI PENIKPDDLC TLVRPPQSTS FPLGLAALAK QKLPGRVRLF NNEKAMLSCF CAMLKVEDPD VIIGHRL QN VYLDVLAHRM HDLNIPTFSS IGRRLRRTWP EKFGRGNSNM NHFFISDICS GRLICDIANE MGQSLTPKCQ SWDLSEMY Q VTCEKEHKPL DIDYQNPQYQ NDVNSMTMAL QENITNCMIS AEVSYRIQLL TLTKQLTNLA GNAWAQTLGG TRAGRNEYI LLHEFSRNGF IVPDKEGNRS RAQKQRQNEE NADAPVNSKK AKYQGGLVFE PEKGLHKNYV LVMDFNSLYP SIIQEFNICF TTVDRNKED IDELPSVPPS EVDQGVLPRL LANLVDRRRE VKKVMKTETD PHKRVQCDIR QQALKLTANS MYGCLGYVNS R FYAKPLAM LVTNKGREIL MNTRQLAESM NLLVVYGDTD SVMIDTGCDN YADAIKIGLG FKRLVNERYR LLEIDIDNVF KK LLLHAKK KYAALTVNLD KNGNGTTVLE VKGLDMKRRE FCPLSRDVSI HVLNTILSDK DPEEALQEVY DYLEDIRIKV ETN NIRIDK YKINMKLSKD PKAYPGGKNM PAVQVALRMR KAGRVVKAGS VITFVITKQD EIDNAADTPA LSVAERAHAL NEVM IKSNN LIPDPQYYLE KQIFAPVERL LERIDSFNVV RLSEALGLDS KKYFRREGGN NNGEDINNLQ PLETTITDVE RFKDT VTLE LSCPSCDKRF PFGGIVSSNY YRVSYNGLQC KHCEQLFTPL QLTSQIEHSI RAHISLYYAG WLQCDDSTCG IVTRQV SVF GKRCLNDGCT GVMRYKYSDK QLYNQLLYFD SLFDCEKNKK QELKPIYLPD DLDYPKEQLT ESSIKALTEQ NRELMET GR SVVQKYLNDC GRRYVDMTSI FDFMLN

UniProtKB: DNA polymerase alpha catalytic subunit A

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Macromolecule #15: Leading strand

MacromoleculeName: Leading strand / type: dna / ID: 15 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 26.092645 KDa
SequenceString: (DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DG)(DG)(DT)(DA)(DA) (DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG)(DA) (DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG)(DA) (DG) (DT)(DG)(DT)(DG)(DT)(DT) ...String:
(DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DG)(DG)(DT)(DA)(DA) (DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG)(DA) (DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG)(DA) (DG) (DT)(DG)(DT)(DG)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT) (DT)(DT)(DT)(DT)

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Macromolecule #16: Lagging strand

MacromoleculeName: Lagging strand / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 32.046451 KDa
SequenceString: (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT) ...String:
(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT) (DA)(DC)(DA)(DC)(DA)(DC)(DT) (DC)(DT)(DC)(DC)(DA)(DA)(DT)(DT)(DC)(DT) (DC)(DT)(DA) (DA)(DT)(DC)(DA)(DC)(DT) (DT)(DA)(DC)(DC)(DA)(DT)(DC)(DA)(DC)(DT) (DT)(DC)(DC)(DT) (DA)(DC)(DT)(DC)(DT) (DA)

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Macromolecule #21: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 21 / Number of copies: 4 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Macromolecule #22: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 22 / Number of copies: 4 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #23: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 23 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 39.2 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 53964
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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