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TitleHigh-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 4052, Year 2023
Publish dateJul 8, 2023
AuthorsFenglin Li / Chun-Feng David Hou / Ravi K Lokareddy / Ruoyu Yang / Francesca Forti / Federica Briani / Gino Cingolani /
PubMed AbstractE217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after ...E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.
External linksNat Commun / PubMed:37422479 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.96 Å
Structure data

28280

8env

EMDB-28280, PDB-8env:
In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C6 map
Method: EM (single particle) / Resolution: 3.42 Å

28405

8eon

EMDB-28405, PDB-8eon:
Pseudomonas phage E217 baseplate complex
Method: EM (single particle) / Resolution: 3.6 Å

29406

8frs

EMDB-29406, PDB-8frs:
Pseudomonas phage E217 5-fold vertex (capsid and decorating proteins)
Method: EM (single particle) / Resolution: 3.96 Å

29481

8fuv

EMDB-29481, PDB-8fuv:
Pseudomonas phage E217 extended sheath and tail tube
Method: EM (single particle) / Resolution: 3.1 Å

29486

8fvg

EMDB-29486, PDB-8fvg:
Pseudomonas phage E217 contracted sheath
Method: EM (single particle) / Resolution: 3.1 Å

29487

8fvh

EMDB-29487, PDB-8fvh:
Pseudomonas phage E217 neck (portal, head-to-tail connector, collar and gateway proteins)
Method: EM (single particle) / Resolution: 3.1 Å

Source
  • pseudomonas phage vb_paem_e217 (virus)
KeywordsVIRUS / Pseudomonas / phage / baseplate / E217 / capsid / decorating proteins

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