EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and topography of the synaptic V-ATPase-synaptophysin complex.
ジャーナル・号・ページ	Nature, Vol. 631, Issue 8022, Page 899-904, Year 2024
掲載日	2024年6月5日
著者	Chuchu Wang / Wenhong Jiang / Jeremy Leitz / Kailu Yang / Luis Esquivies / Xing Wang / Xiaotao Shen / Richard G Held / Daniel J Adams / Tamara Basta / Lucas Hampton / Ruiqi Jian / Lihua Jiang / Michael H B Stowell / Wolfgang Baumeister / Qiang Guo / Axel T Brunger /
PubMed 要旨	Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a ...Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a well-defined interface between the synaptic vesicle V-ATPase and synaptophysin by in situ cryo-electron tomography and single-particle cryo-electron microscopy of functional synaptic vesicles isolated from mouse brains. The synaptic vesicle V-ATPase is an ATP-dependent proton pump that establishes the proton gradient across the synaptic vesicle, which in turn drives the uptake of neurotransmitters. Synaptophysin and its paralogues synaptoporin and synaptogyrin belong to a family of abundant synaptic vesicle proteins whose function is still unclear. We performed structural and functional studies of synaptophysin-knockout mice, confirming the identity of synaptophysin as an interaction partner with the V-ATPase. Although there is little change in the conformation of the V-ATPase upon interaction with synaptophysin, the presence of synaptophysin in synaptic vesicles profoundly affects the copy number of V-ATPases. This effect on the topography of synaptic vesicles suggests that synaptophysin assists in their biogenesis. In support of this model, we observed that synaptophysin-knockout mice exhibit severe seizure susceptibility, suggesting an imbalance of neurotransmitter release as a physiological consequence of the absence of synaptophysin.
リンク	Nature / PubMed:38838737 / PubMed Central
手法	EM (単粒子) / EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	3.6 - 18.2 Å
構造データ	44839 9bra EMDB-44839, PDB-9bra: Intact V-ATPase State 2 and synaptophysin complex in mouse brain isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.3 Å 44840 9brq EMDB-44840, PDB-9brq: Intact V-ATPase State 3 and synaptophysin complex in mouse brain isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.3 Å 44841 9brr EMDB-44841, PDB-9brr: Intact V-ATPase State 3 in synaptophysin knock-out isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.5 Å 44842 9brs EMDB-44842, PDB-9brs: Intact V-ATPase State 2 in synaptophysin knock-out isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.4 Å 44843 9brt EMDB-44843, PDB-9brt: Intact V-ATPase State 1 and synaptophysin complex in mouse brain isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.3 Å 44844 9bru EMDB-44844, PDB-9bru: Intact V-ATPase State 1 in synaptophysin knock-out isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 4.4 Å 44845 9bry EMDB-44845, PDB-9bry: V0-only V-ATPase in synaptophysin gene knock-out mouse brain isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 3.6 Å 44846 9brz EMDB-44846, PDB-9brz: V0-only V-ATPase and synaptophysin complex in mouse brain isolated synaptic vesicles 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-44847: Tomograms of isolated synaptic vesicles from Syp-/- mouse brain 手法: EM (トモグラフィー) EMDB-44848: Tomograms of isolated synaptic vesicles from wild-type mouse brain 手法: EM (トモグラフィー) EMDB-44855: Intact state1 V-ATPase of isolated synaptic vesicles from wild-type mouse brain by subtomogram averaging 手法: EM (サブトモグラム平均) / 解像度: 18.2 Å EMDB-44856: Intact state2 V-ATPase of isolated synaptic vesicles from wild-type mouse brain by subtomogram averaging 手法: EM (サブトモグラム平均) / 解像度: 15.9 Å EMDB-44857: Intact state3 V-ATPase of isolated synaptic vesicles from wild-type mouse brain by subtomogram averaging 手法: EM (サブトモグラム平均) / 解像度: 17.1 Å EMDB-44858: V0-only V-ATPase of isolated synaptic vesicles from wild-type mouse brain by subtomogram averaging 手法: EM (サブトモグラム平均) / 解像度: 16.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-BMA: beta-D-mannopyranose / β-D-マンノピラノ-ス
由来	mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN / V-ATPase / synaptic vesicle / V0only V-ATPase