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- EMDB-44839: Intact V-ATPase State 2 and synaptophysin complex in mouse brain ... -
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Basic information
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Title | Intact V-ATPase State 2 and synaptophysin complex in mouse brain isolated synaptic vesicles | |||||||||
![]() | Full map of V-ATPase State 2 synaptophysin complex in wild-type ISVs. | |||||||||
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![]() | V-ATPase / synaptic vesicle / MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() Ion channel transport / regulation of opioid receptor signaling pathway / Amino acids regulate mTORC1 / clathrin-sculpted glutamate transport vesicle membrane / Transferrin endocytosis and recycling / Insulin receptor recycling / eye pigmentation / RHOA GTPase cycle / central nervous system maturation / Metabolism of Angiotensinogen to Angiotensins ...Ion channel transport / regulation of opioid receptor signaling pathway / Amino acids regulate mTORC1 / clathrin-sculpted glutamate transport vesicle membrane / Transferrin endocytosis and recycling / Insulin receptor recycling / eye pigmentation / RHOA GTPase cycle / central nervous system maturation / Metabolism of Angiotensinogen to Angiotensins / transporter activator activity / negative regulation of autophagic cell death / plasma membrane proton-transporting V-type ATPase complex / rostrocaudal neural tube patterning / regulation of synaptic vesicle priming / cellular response to increased oxygen levels / positive regulation of transforming growth factor beta1 production / proton-transporting V-type ATPase, V1 domain / synaptic vesicle lumen acidification / intracellular organelle / ROS and RNS production in phagocytes / proton-transporting V-type ATPase, V0 domain / extrinsic component of synaptic vesicle membrane / P-type proton-exporting transporter activity / endosome to plasma membrane protein transport / lysosomal lumen acidification / clathrin-coated vesicle membrane / endosomal lumen acidification / vacuolar proton-transporting V-type ATPase, V0 domain / vacuolar proton-transporting V-type ATPase, V1 domain / vacuolar transport / proton-transporting V-type ATPase complex / neuron spine / head morphogenesis / osteoclast development / regulation of short-term neuronal synaptic plasticity / protein localization to cilium / neuron projection terminus / vacuolar proton-transporting V-type ATPase complex / dendritic spine membrane / regulation of cellular pH / syntaxin-1 binding / vacuolar acidification / cholesterol binding / : / ATPase complex / regulation of neuronal synaptic plasticity / presynaptic active zone / microvillus / autophagosome membrane / regulation of MAPK cascade / ATPase activator activity / synaptic vesicle endocytosis / excitatory synapse / positive regulation of Wnt signaling pathway / cilium assembly / transmembrane transporter complex / endomembrane system / regulation of macroautophagy / angiotensin maturation / ATP metabolic process / axon terminus / H+-transporting two-sector ATPase / ruffle / RNA endonuclease activity / proton-transporting ATPase activity, rotational mechanism / Neutrophil degranulation / proton transmembrane transport / endoplasmic reticulum-Golgi intermediate compartment membrane / proton-transporting ATP synthase activity, rotational mechanism / SH2 domain binding / receptor-mediated endocytosis / SNARE binding / secretory granule / regulation of long-term neuronal synaptic plasticity / modulation of chemical synaptic transmission / neuromuscular junction / Schaffer collateral - CA1 synapse / terminal bouton / cilium / transmembrane transport / small GTPase binding / synaptic vesicle membrane / endocytosis / positive regulation of canonical Wnt signaling pathway / melanosome / synaptic vesicle / apical part of cell / myelin sheath / presynapse / signaling receptor activity / presynaptic membrane / ATPase binding / cell body / chemical synaptic transmission / postsynaptic membrane / intracellular iron ion homeostasis / receptor-mediated endocytosis of virus by host cell / Hydrolases; Acting on ester bonds / positive regulation of ERK1 and ERK2 cascade Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | |||||||||
![]() | Wang C / Jiang W / Yang K / Wang X / Guo Q / Brunger AT | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and topography of the synaptic V-ATPase-synaptophysin complex. Authors: Chuchu Wang / Wenhong Jiang / Jeremy Leitz / Kailu Yang / Luis Esquivies / Xing Wang / Xiaotao Shen / Richard G Held / Daniel J Adams / Tamara Basta / Lucas Hampton / Ruiqi Jian / Lihua ...Authors: Chuchu Wang / Wenhong Jiang / Jeremy Leitz / Kailu Yang / Luis Esquivies / Xing Wang / Xiaotao Shen / Richard G Held / Daniel J Adams / Tamara Basta / Lucas Hampton / Ruiqi Jian / Lihua Jiang / Michael H B Stowell / Wolfgang Baumeister / Qiang Guo / Axel T Brunger / ![]() ![]() ![]() ![]() Abstract: Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a ...Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here we discovered a well-defined interface between the synaptic vesicle V-ATPase and synaptophysin by in situ cryo-electron tomography and single-particle cryo-electron microscopy of functional synaptic vesicles isolated from mouse brains. The synaptic vesicle V-ATPase is an ATP-dependent proton pump that establishes the proton gradient across the synaptic vesicle, which in turn drives the uptake of neurotransmitters. Synaptophysin and its paralogues synaptoporin and synaptogyrin belong to a family of abundant synaptic vesicle proteins whose function is still unclear. We performed structural and functional studies of synaptophysin-knockout mice, confirming the identity of synaptophysin as an interaction partner with the V-ATPase. Although there is little change in the conformation of the V-ATPase upon interaction with synaptophysin, the presence of synaptophysin in synaptic vesicles profoundly affects the copy number of V-ATPases. This effect on the topography of synaptic vesicles suggests that synaptophysin assists in their biogenesis. In support of this model, we observed that synaptophysin-knockout mice exhibit severe seizure susceptibility, suggesting an imbalance of neurotransmitter release as a physiological consequence of the absence of synaptophysin. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 322.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 38.9 KB 38.9 KB | Display Display | ![]() |
Images | ![]() | 128.9 KB | ||
Filedesc metadata | ![]() | 9.9 KB | ||
Others | ![]() ![]() ![]() ![]() | 318.5 MB 274 MB 318.1 MB 318.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9braMC ![]() 9brqC ![]() 9brrC ![]() 9brsC ![]() 9brtC ![]() 9bruC ![]() 9bryC ![]() 9brzC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Full map of V-ATPase State 2 synaptophysin complex in wild-type ISVs. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.11138 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: EMhancer map of V-ATPase State 2 synaptophysin complex...
File | emd_44839_additional_1.map | ||||||||||||
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Annotation | EMhancer map of V-ATPase State 2 synaptophysin complex in wild-type ISVs. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Unsharpened map of V-ATPase State 2
File | emd_44839_additional_2.map | ||||||||||||
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Annotation | Unsharpened map of V-ATPase State 2 | ||||||||||||
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Density Histograms |
-Half map: Half map of V-ATPase State 2 synaptophysin complex in wild-type ISVs.
File | emd_44839_half_map_1.map | ||||||||||||
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Annotation | Half map of V-ATPase State 2 synaptophysin complex in wild-type ISVs. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map of V-ATPase State 2 synaptophysin complex in wild-type ISVs.
File | emd_44839_half_map_2.map | ||||||||||||
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Annotation | Half map of V-ATPase State 2 synaptophysin complex in wild-type ISVs. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Mouse brain isolated glutamatergic synaptic vesicles
+Supramolecule #1: Mouse brain isolated glutamatergic synaptic vesicles
+Macromolecule #1: V-type proton ATPase subunit E 1
+Macromolecule #2: V-type proton ATPase subunit G 2
+Macromolecule #3: V-type proton ATPase catalytic subunit A
+Macromolecule #4: V-type proton ATPase subunit B, brain isoform
+Macromolecule #5: V-type proton ATPase subunit C 1
+Macromolecule #6: V-type proton ATPase subunit D
+Macromolecule #7: V-type proton ATPase subunit H
+Macromolecule #8: V-type proton ATPase subunit F
+Macromolecule #9: V-type proton ATPase 116 kDa subunit a 1
+Macromolecule #10: V-type proton ATPase 21 kDa proteolipid subunit c''
+Macromolecule #11: V-type proton ATPase subunit d 1
+Macromolecule #12: V-type proton ATPase 16 kDa proteolipid subunit c
+Macromolecule #13: Renin receptor cytoplasmic fragment
+Macromolecule #14: V-type proton ATPase subunit S1
+Macromolecule #15: Ribonuclease kappa
+Macromolecule #16: Synaptophysin
+Macromolecule #17: V-type proton ATPase subunit e 2
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
Details | The specimen state should be an intact subcellular component. |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 25667 |
Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Refinement | Protocol: FLEXIBLE FIT |
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Output model | ![]() PDB-9bra: |