National Institutes of Health/National Human Genome Research Institute
R01 GM047795
United States
National Institutes of Health/National Human Genome Research Institute
S10 OD019995
United States
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2019 Title: Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy. Authors: Alexis Huet / Robert L Duda / Pascale Boulanger / James F Conway / Abstract: The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a ...The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a regulated process that involves several intermediates, including a thick-walled round precursor prohead that expands as the viral DNA is packaged to yield a thin-walled and angular mature capsid. We investigated capsid maturation by comparing cryoelectron microscopy (cryo-EM) structures of the prohead, the empty expanded capsid both with and without decoration protein, and the virion capsid at a resolution of 3.8 Å for the latter. We detail the molecular structure of the mcp, its complex pattern of interactions, and their evolution during maturation. The bacteriophage T5 mcp is a variant of the canonical HK97-fold with a high level of plasticity that allows for the precise assembly of a giant macromolecule and the adaptability needed to interact with other proteins and the packaged DNA.
History
Deposition
Apr 18, 2019
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Header (metadata) release
Jul 24, 2019
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Map release
Oct 2, 2019
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Update
Oct 9, 2019
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Current status
Oct 9, 2019
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Shell ID: 1 / Name: D-head / Diameter: 700.0 Å / T number (triangulation number): 13
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Experimental details
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Structure determination
Method
cryo EM
Processing
single particle reconstruction
Aggregation state
particle
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Sample preparation
Concentration
2 mg/mL
Buffer
pH: 7.6 Component:
Concentration
Formula
Name
100.0 mM
NaCl
Sodium chloride
10.0 mM
Tris
1.0 mM
MGCl2
1.0 mM
CaCL2
Grid
Details: unspecified
Vitrification
Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK III
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Electron microscopy
Microscope
FEI POLARA 300
Image recording
Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 1 / Number real images: 1939 / Average electron dose: 30.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD
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