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- EMDB-0232: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -

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Basic information

Entry
Database: EMDB / ID: EMD-0232
TitleCryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the body of the small mitoribosomal subunit in complex with mt-IF-3
Map datamap of the T. brucei mitoribosome small subunit body in complex with mt-IF3
Sample
  • Complex: body of the T. brucei mitoribosome small subunit in complex with mt-IF-3
    • Protein or peptide: x 40 types
    • RNA: x 1 types
  • Ligand: x 4 types
Function / homology
Function and homology information


modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / kinetoplast / regulation of protein kinase A signaling / quorum sensing / enoyl-CoA hydratase activity ...modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / kinetoplast / regulation of protein kinase A signaling / quorum sensing / enoyl-CoA hydratase activity / ciliary plasm / mRNA stabilization / mitochondrial small ribosomal subunit / fatty acid beta-oxidation / superoxide dismutase / protein kinase A regulatory subunit binding / superoxide dismutase activity / ribosome / structural constituent of ribosome / translation / mitochondrion / RNA binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Nucleoid-associated protein YbaB-like domain superfamily / : / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / : / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase ...Nucleoid-associated protein YbaB-like domain superfamily / : / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / : / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Cyclic phosphodiesterase / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / ClpP/crotonase-like domain superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S5, C-terminal domain / Ubiquitin family / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S9 / Ribosomal protein S9/S16 / Ubiquitin domain profile. / Ribosomal protein S17/S11 / Ribosomal protein S17 / Ubiquitin-like domain / Ribosomal protein S11 superfamily / S15/NS1, RNA-binding / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold
Similarity search - Domain/homology
A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Uncharacterized protein / Probable Zinc-ribbon domain-containing protein / 30S ribosomal protein S8, putative / Small ribosomal subunit protein mS41 / Uncharacterized protein / Mitochondrial ribosomal protein S18 ...A-kinase anchor protein 7-like phosphoesterase domain-containing protein / Uncharacterized protein / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Uncharacterized protein / Probable Zinc-ribbon domain-containing protein / 30S ribosomal protein S8, putative / Small ribosomal subunit protein mS41 / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Small ribosomal subunit protein uS5 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Trans-sialidase / Uncharacterized protein / 30S Ribosomal protein S17, putative / Uncharacterized protein / Opioid growth factor receptor (OGFr) conserved domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S23 mitochondrial conserved domain-containing protein / Uncharacterized protein / Ribosomal protein S9 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ubiquitin-like domain-containing protein / Manganese/iron superoxide dismutase C-terminal domain-containing protein / Superoxide dismutase, putative / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesTrypanosoma brucei brucei (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.27 Å
AuthorsRamrath DJF / Niemann M / Leibundgut M / Bieri P / Prange C / Horn EK / Leitner A / Boehringer D / Schneider A / Ban N
Funding support Switzerland, 1 items
OrganizationGrant numberCountry
Swiss National Science Foundation Switzerland
CitationJournal: Science / Year: 2018
Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
History
DepositionAug 31, 2018-
Header (metadata) releaseSep 26, 2018-
Map releaseSep 26, 2018-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6hiy
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0232.png

Downloads & links

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Map

FileDownload / File: emd_0232.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmap of the T. brucei mitoribosome small subunit body in complex with mt-IF3
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.39 Å/pix.
x 320 pix.
= 444.8 Å		1.39 Å/pix.
x 320 pix.
= 444.8 Å		1.39 Å/pix.
x 320 pix.
= 444.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.39 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0115 / Movie #1: 0.15
Minimum - Maximum-0.26455936 - 0.51369876
Average (Standard dev.)0.0014350519 (±0.017512508)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 444.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z444.800444.800444.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.2650.5140.001

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Supplemental data

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Half map: half map (even) of the T. brucei mitoribosome...

Fileemd_0232_half_map_1.map
Annotationhalf map (even) of the T. brucei mitoribosome small subunit body in complex with mt-IF3
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map (odd) of the T. brucei mitoribosome...

Fileemd_0232_half_map_2.map
Annotationhalf map (odd) of the T. brucei mitoribosome small subunit body in complex with mt-IF3
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : body of the T. brucei mitoribosome small subunit in complex with ...

EntireName: body of the T. brucei mitoribosome small subunit in complex with mt-IF-3
Components
  • Complex: body of the T. brucei mitoribosome small subunit in complex with mt-IF-3
    • Protein or peptide: mS48
    • Protein or peptide: mS51
    • Protein or peptide: mS56
    • Protein or peptide: mS59
    • Protein or peptide: mS60
    • Protein or peptide: mS61
    • Protein or peptide: mS62
    • Protein or peptide: mS63
    • Protein or peptide: mS64
    • Protein or peptide: mS65
    • Protein or peptide: mS66
    • Protein or peptide: mS68
    • Protein or peptide: mS73
    • Protein or peptide: mS74
    • Protein or peptide: mS55m
    • Protein or peptide: bS6m
    • Protein or peptide: uS8m
    • Protein or peptide: uS9m
    • Protein or peptide: uS11m
    • Protein or peptide: uS12m
    • Protein or peptide: uS15m
    • Protein or peptide: bS16m
    • Protein or peptide: uS17m
    • Protein or peptide: bS18m
    • Protein or peptide: uS21m
    • Protein or peptide: mt-IF-3
    • Protein or peptide: mS22
    • Protein or peptide: mS23
    • Protein or peptide: mS26
    • Protein or peptide: mS34
    • Protein or peptide: mS37
    • Protein or peptide: mS38
    • Protein or peptide: mS41
    • Protein or peptide: mS42
    • Protein or peptide: mS43
    • Protein or peptide: mS47
    • RNA: 9S rRNA
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: SPERMIDINE
  • Ligand: SPERMINE

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Supramolecule #1: body of the T. brucei mitoribosome small subunit in complex with ...

SupramoleculeName: body of the T. brucei mitoribosome small subunit in complex with mt-IF-3
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Molecular weightExperimental: 1.35 MDa

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Macromolecule #1: mS48

MacromoleculeName: mS48 / type: protein_or_peptide / ID: 1 / Details: residues 1470-1487 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 201.811188 KDa
SequenceString: MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL ...String:
MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL YQAGAHGAQL SNPRSPFTAK VLGDELMQVR DIKSVEHCKA WFDRLQYLIN LHYEAVGDIG EFKSRHTRHV HE FFVAFHD ALSSLDFRDT YLFDQFKAVR PPELSDLFGI FLEMEANYVH EDYCPRCSLP YSTTRYCGEG DVDTPFRKHR GRW APHQKW GREWYDVVAR RAEALWYRAT EDPYFGTPQH TQRQAEALLK VYVQAKQRGK AIDFMNKLRG SVEYLTGDIT ITTV MQESY DALLDTTPHP HLLTNGFTLE SDAAKYTGEV NKAPLSPLQF RIDMEMNKYR RQQKEEGVVR VPPALWRLDT SAIVP YKVD SKTRRIVNWR EVKEGIEKSF LSVGLPKEAY TGNEWREMLY LHDVIANREA KAAVLEQQQK LDREKLKARE RASLKG EKS SNSASNGIGI IFPDKDGYQV FFVDEASLRP FGIGASGTLF KAVARVYPSP AAVPYDDPVH GKQFLIDTTD ETCHLFG GF EHGDRLLLRA KSSGGDVDGG NNSNCRNTGS FTDEEEEVIV MGVSTEGSDA ERMLCAMHVD TGKQRERGIV FLGTDCID I RERWESVRYA ASAYVKGRVT LLESQRTARE EFMGQVVGIR DGVLFVQWRL LKGGGSVLDR SVAVPIGDST QVRELFVET KVLGVEPLQS PPSWRTPFRN DFAEERLKEL QRAPFKREKY VSLIQGKYTP KVKKFGYTQH TTVDDFETKE YKDRLLSKQF FQNPQAFEV VPDRHERSVQ FGGKWEYQRT HGLPTVDRNE LENGWSEVEP ISDGEMKVIE QALRDISGPR PGNFVKPPSK T KSLQLSES WWEPLGYGWE QHNEEQKALC DVTEQRLIDG SNLPFGGKLP PFGTSYGMGE RMRAIIEDYS KGFGLGPHGH SP THDTIHY NTLNAEGERV RDLGYTDALG RLFSEKLGDR DVHQWAVESC ADGEADVRQL LLSLHEWRER GRPPSLLLAN VLS KYLEEE IAAFNKGIPE NAPKLQLETS DGTLAHSGSG GSRSGTMWAD VDPTTFALHQ ASQSTRSTRC DEPFILHLVK RAKL GECVT NFTDTAYIAH LESSVINEFH LALTKLVGKG ISPTLLAQKT GQLHRGSVRV GGNVVPFVKS RELSRLLERM GLTSE SIAV ITRGLANCPE QESVGDDFAV PVSLILSWDG PGSSGGSGSG DRRLNTTAAR NEQQRKGSAA LSSAIRQLSQ HQKQRG SAS GRGWQNEDKM VVHVLEELSL RNDGLVMDIQ YAIRENKKNR RLRWEFVTTL LPVFGGNEAK VEQLYSDYCD GKYVPNI TV AVEAFIAFLH NATQHPETYS ASDYFDIDDG KSSTASGTGD QPSAGQYTTL KLLDPLEGPF VFDNVKVEYI QTVERFRR H GIRAGPVMAP ATGFIAANCK SLNYFTRRQE EVVYVTNDSD QGLRRSLENS AYHKTIAANP ALQYLLKARR GAALVETFN RFFYRTMPML NFYQNVLKHY SETIQPLRQA AKSSTRGLAR ALESERSAAM EEFRRNSERY WRNIIEGRSV EQVVLANGES NRRTVGGGA GEQLQKPQGN RDAVRTNFTG EATDGSRKGQ RQHQQPPLSQ QQQHSRGEGE RSAVHTSPRQ GGSRGMADLL S KLNTPKTG SGVKGSTAKN TGNNRRGPKS

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Macromolecule #2: mS51

MacromoleculeName: mS51 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 95.080992 KDa
SequenceString: MLRCTVVGHH RSGKARAFVF RDPSLRMMRA GSGYQQLRRM GMPMQVGMGW RKVDSFHANT QYQHAWPLLS HDDLGNSDQS NNTKNIMYS MYMPKRNKGT APWFRGADTY SVKYCEQGRY EYQRYLMINR FPSEYKKHFL SFLSNIRMSS GSATIPQEAL H WLLRMIVD ...String:
MLRCTVVGHH RSGKARAFVF RDPSLRMMRA GSGYQQLRRM GMPMQVGMGW RKVDSFHANT QYQHAWPLLS HDDLGNSDQS NNTKNIMYS MYMPKRNKGT APWFRGADTY SVKYCEQGRY EYQRYLMINR FPSEYKKHFL SFLSNIRMSS GSATIPQEAL H WLLRMIVD NFNPQHVHYI AAMKTLQSAG ELDMARDVWK IMERQQTWPC TATICAYLDV CVEAGEKTWA MEAWNRYCTE LK FLEPGEV DPKPISRVPF SLTREELLYL PKWKKHFDHD PNLDVMDLNR FNRTREVYLR MAQVMLAGGE RNAFQHFFTK LEE AMLNKP TPVPEPPNPH LVRRPRWAPY EHCKSVHHSP WRLQNNGRAL ALGPPVTIED EMQSRFFSND QFLVHSVKEV LRIV LQEHK RAHPTECTRC KTEAFFYKTK DADETLKFCD DLIERLFASL GVRLSNLNTS SLLSTILEVF RVVGKESGAA LLQRA NEFL ERKASLGDAE GSRENLTASN YLQVLSGFAD ESAFVYNTKK DGTCQYKTGF DPRTTMRHLA DVVQEIAGNP HVTWAA DMH LQVVETMVGC GTMKANDYFV RNVLRQFSWD SRFLEALYVE YRRQDDVDMW AELTKRALVW TARYNAPASE RLRRLIE DD YDTIRVQTRT FRELAVFQFR DVEERRHSRD VVNELPNPWY DYVAHALPFP DRDAGYPDEY GDLGQWRAPG GPGSPVRG P GYYAPPMEGE HQRGYTAEWR DLRNPMRPPE FPTPWERKYR QYARGQHPSY DMVYAGPMPE IFPMRRDFRK PTRWDFHDI EKQGKYRTSG PY

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Macromolecule #3: mS56

MacromoleculeName: mS56 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 47.306836 KDa
SequenceString: MRKFCHFMAN CWNSARSHAT YGAVPLTHSQ VTSVYATDGG KVDELGLLEL VEERIFSWKL NKWEMRIPPN LPNDQKELIR QEQENLKQI LSEWRKCFGA LNADILQISS LTGVPKDVVR EKNRTWLQEE VAKLRWMGEV NKAALLRDAF MRLEAFGSRD F MFMERLCC ...String:
MRKFCHFMAN CWNSARSHAT YGAVPLTHSQ VTSVYATDGG KVDELGLLEL VEERIFSWKL NKWEMRIPPN LPNDQKELIR QEQENLKQI LSEWRKCFGA LNADILQISS LTGVPKDVVR EKNRTWLQEE VAKLRWMGEV NKAALLRDAF MRLEAFGSRD F MFMERLCC IYGLARQGTF DEAFTNYITE DPVTNDIFVD ERNPFKELVA HIVRNYSQID IIYDFLGFNY SEGYRSSLRR YM EYLQCKT AENVRASGRL VTGDKGEHNI LFDYCVSRES LVSGDSCQGI IDFLYINGND VTLIIIASDN PWLRNRQLPH RRQ MEGIAR RVCFVLGIPP SEVRIRNLLL PPTYLDKGSI VRLNDIVFRL SNEQSNLLIP WLTNYNKELD PKDVDYTALA KTTN EEEWL TL

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Macromolecule #4: mS59

MacromoleculeName: mS59 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 35.395676 KDa
SequenceString: MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES ...String:
MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES AVRSPAYREI AKRQLEGGAA TRLAGISAAA DVMRRMGLTR RPMSSYFELY QRIVERSEAM TPWGFPPLFQ FE ERLSLEP RLKFFSRASQ QSLERRRRGH VMTPHTTLHG RRIFWIPPTW NRAGRFLGPH VTLYPGMTPD

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Macromolecule #5: mS60

MacromoleculeName: mS60 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 34.546641 KDa
SequenceString: MEVYRRITER VRGALWAHHG RLMSERQFHR ILAFVYNNKY EYQIRFDRME VVGREWGGRV EVVTTPAGFL KRVRVNPCVE DLTLYQQQQ LILSAYANAC ADGRKLMEQA EMNIYKQFLK DLKPIVMGIR DNPEFYTVSE NSIETIGGVL QTGDSNNLCH T HRTIPAAK ...String:
MEVYRRITER VRGALWAHHG RLMSERQFHR ILAFVYNNKY EYQIRFDRME VVGREWGGRV EVVTTPAGFL KRVRVNPCVE DLTLYQQQQ LILSAYANAC ADGRKLMEQA EMNIYKQFLK DLKPIVMGIR DNPEFYTVSE NSIETIGGVL QTGDSNNLCH T HRTIPAAK ARQPADEVRA RQEWEQKWIN SPRGQSWALT LRGKTYFALH GPQYRPRGAP GAKKETLPLD IPAPYTAMDE KR LLKKNWM AYLDNKHVAE VMWTRVKIAD REKRQRLLQE TGRAWHRPIN EEAINRW

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Macromolecule #6: mS61

MacromoleculeName: mS61 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 33.48234 KDa
SequenceString: MLCRTFLRQF RMSGGDMFVE YKVLSRDHRR SIRVEDAIVD PTFKRTVLPL GWLELLRSPS LRLPTGYFVE ETVHVSLPNA TSNGGKKEA RPQKGGFASG SPSVGRNEAN AIIAGPVVLY ITGQSVPVVL NPYFVPEGTW DMRTRDGELD LRLGMDAIEQ C TLFSELRP ...String:
MLCRTFLRQF RMSGGDMFVE YKVLSRDHRR SIRVEDAIVD PTFKRTVLPL GWLELLRSPS LRLPTGYFVE ETVHVSLPNA TSNGGKKEA RPQKGGFASG SPSVGRNEAN AIIAGPVVLY ITGQSVPVVL NPYFVPEGTW DMRTRDGELD LRLGMDAIEQ C TLFSELRP GGLLYGKLPE NPNVRRNESL RATLGRYGMK CDLAESPLVP RPWTRMRYMF IDELQRGPKL TEFVGHNPRN GT PWRFSQN TKYFRIGIWR DTIRRNDMNE GLHAHSSWQK SPQQSVPEVR FLAPYP

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Macromolecule #7: mS62

MacromoleculeName: mS62 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 32.0681 KDa
SequenceString: MRRFCTARVS SYVKRAPLLL PRGGRRWRSG AGTTADGGGE KEYIADSFES TAGSNAYAAM ELAAEARREI HELWLSAETA LEREKRVQQ VAALIEKYKL DPSTPREADV SRGLGDAFDR LLLLCLPLGK TDAKGTDNLE RLMHLAGRNG RELSVRTIQH L FARTDSFA ...String:
MRRFCTARVS SYVKRAPLLL PRGGRRWRSG AGTTADGGGE KEYIADSFES TAGSNAYAAM ELAAEARREI HELWLSAETA LEREKRVQQ VAALIEKYKL DPSTPREADV SRGLGDAFDR LLLLCLPLGK TDAKGTDNLE RLMHLAGRNG RELSVRTIQH L FARTDSFA EALAVFYTMR RCHVAMNMEA YYSMLYSLQR LEEEGWGQHF RNEYEENGAP SEQAMDFIVK GISNALLPEN KP WLGRVMF QDRNVPDRRY DTRDFDELDT AWTQRYKSGT PAGAH

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Macromolecule #8: mS63

MacromoleculeName: mS63 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 31.922178 KDa
SequenceString: MLHGTPVRRA SLRYRRPYWM MFLKGVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHMLRK ERLDGPETP LEKYVLEWHK KFHSFQGTER PTPDDLHTAL DLVERPLDLS YALQLLGQCR NLNNIRFAKE TFLVFLEACL R VGRRDCAE ...String:
MLHGTPVRRA SLRYRRPYWM MFLKGVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHMLRK ERLDGPETP LEKYVLEWHK KFHSFQGTER PTPDDLHTAL DLVERPLDLS YALQLLGQCR NLNNIRFAKE TFLVFLEACL R VGRRDCAE YALEHAEPLG FWFIDEDHRR YLQGEQTWYK LSPLDNLYYP VEENAKLNEG RKPITRLSPA TESPGSGTAI SD GEPSTDV EGETTVDDEI AQLEAELAAL EREGGGK

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Macromolecule #9: mS64

MacromoleculeName: mS64 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 30.742207 KDa
SequenceString: MLRRVPRVLQ KGERGLYFPI NHRIVDHRIA PGVTVEETDV QSRYRRELRT NFATGETRQT IAPPWSARER PTHFFSLRLP SRNSVTLRV KEMHNHLLFS HQQCAPMLVS PTKLHITLGV MTIPENSREE LVKNIQQSMS ETFACVQPLQ LRFRGLGTFD F GRVLFIRV ...String:
MLRRVPRVLQ KGERGLYFPI NHRIVDHRIA PGVTVEETDV QSRYRRELRT NFATGETRQT IAPPWSARER PTHFFSLRLP SRNSVTLRV KEMHNHLLFS HQQCAPMLVS PTKLHITLGV MTIPENSREE LVKNIQQSMS ETFACVQPLQ LRFRGLGTFD F GRVLFIRV VPEADFGRLE RAVCDIRRRV GGVLGVDLKG NPHDSYVPHV TVAKIRPNQR QQLGDTIPQS LWADYQHHDF GD VTLSTVD LCRMKGSPDG YYHTEGAVHL S

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Macromolecule #10: mS65

MacromoleculeName: mS65 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 30.617381 KDa
SequenceString: MFSESLCILS RRFRYNTKFP ALVSYNKLPW EVVNHETPQF HMHVAPHYEQ LLTLAASSPV PHIIGSKHID VPREHRLRLL PGMLYLLDG DTLPGEFTIN RVLDPTALQY YGRLSSQIVT VEAVRMLVPD DLRLLCNCIT FKGPLHLPVA PYASLASLRG A SQGGTTGS ...String:
MFSESLCILS RRFRYNTKFP ALVSYNKLPW EVVNHETPQF HMHVAPHYEQ LLTLAASSPV PHIIGSKHID VPREHRLRLL PGMLYLLDG DTLPGEFTIN RVLDPTALQY YGRLSSQIVT VEAVRMLVPD DLRLLCNCIT FKGPLHLPVA PYASLASLRG A SQGGTTGS ETGSNCFTLY HFVRPNRPPK ELQLEKYYIH APCVAPLSEF ASNSDERGNW RPRLQAPKRT RRATPLPAYR PP QSYLMGL AERLAVVPGG CFGRRSLMWG HWF

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Macromolecule #11: mS66

MacromoleculeName: mS66 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 29.960182 KDa
SequenceString: MFNRNFILRA AEFKQKSRWS SVWPNMRYGS MFLSYSVGRK LPMKGVNWVT RDSNRLLNFS NRYSAVISDI DVKRTEEELN VALSDIRWN DHRRIYWKCS FCGSSYRKNV SVRTKYHAGC NFCKKKYPSE VRGEQHGSPP VSQAAPELTE QLVDNGKRDN L ATLAVTSK ...String:
MFNRNFILRA AEFKQKSRWS SVWPNMRYGS MFLSYSVGRK LPMKGVNWVT RDSNRLLNFS NRYSAVISDI DVKRTEEELN VALSDIRWN DHRRIYWKCS FCGSSYRKNV SVRTKYHAGC NFCKKKYPSE VRGEQHGSPP VSQAAPELTE QLVDNGKRDN L ATLAVTSK FYAEWTCRGC GGSYRSTIRS RTGQVEAGQC TLHPDIVAWS AYCPSCSWRP NMVPIAEEVS RSGQFLGLER SM EELGGQR GKQLPIPRRK RLAC

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Macromolecule #12: mS68

MacromoleculeName: mS68 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 26.472807 KDa
SequenceString: MRRGALSLLK AGLLGHYQQE AFEARKRFEE STTYPGPIRA ATPGDTRFYS GSLESILHDT DRHYWRAVTD DPRVQHLIPL RIRFKIFTW VTSGWEQRMQ VVQIMAPKDS TIAQVKDLVI VENQSPYLCV SSFHLAIDGK ELDPQKTLGE YGITEQSQID A IEQNDHLL ...String:
MRRGALSLLK AGLLGHYQQE AFEARKRFEE STTYPGPIRA ATPGDTRFYS GSLESILHDT DRHYWRAVTD DPRVQHLIPL RIRFKIFTW VTSGWEQRMQ VVQIMAPKDS TIAQVKDLVI VENQSPYLCV SSFHLAIDGK ELDPQKTLGE YGITEQSQID A IEQNDHLL HRDDERPRDW TVDEITAEDV KRSPYKEMEM QPLQNLAPRY EARPKGYFGR TYYSGMKQSS

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Macromolecule #13: mS73

MacromoleculeName: mS73 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 11.360885 KDa
SequenceString:
MLRRSILFRM KYADLELTTR GEFPHGMKEP AFVKKLDQNI PWYFSTYRSM YHWPITGDNW SDLNEAEKHH DLHMFYTLAW WKLGEGIFG VDEDS

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Macromolecule #14: mS74

MacromoleculeName: mS74 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 8.203408 KDa
SequenceString:
MFSLTQTWLI AHWYCGHKFR HRFMRDKRFH PSLQASHDAR NRFSKRRHFK TNRWNYQQAY RDMP

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Macromolecule #15: mS55m

MacromoleculeName: mS55m / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 50.147355 KDa
SequenceString: MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY ...String:
MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP ARRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKRGYYDD VYWKDFFAGS DEHLNEPRMG LRGDEMRRRL EEAQTSPAPT TAKDTRRRTL EDVL KRLGK TTRDLGSIPI VNPRVDVGLP THIKRNYQLH

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Macromolecule #16: bS6m

MacromoleculeName: bS6m / type: protein_or_peptide / ID: 16 / Details: residues 105-114 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 18.861543 KDa
SequenceString:
MVFYSFVLVM KPRQRRFTSQ ALREIGVAVY SNGGLIRSIT NEGIMRPYSR FRDADNTPLT YARYIILQLD MGEEEMGKVD KIIREHQDV LMALKLNNLE RPVGIRSGNK ELQAAYFPLD TFTRLEEEIN WSPQTSADIY TQLEMNWKEF SRTRWSSFLR N

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Macromolecule #17: uS8m

MacromoleculeName: uS8m / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 32.766459 KDa
SequenceString: MLRQSLLSLA KVYGAVPPLG AAQGHRLLHG KREREGSLFA VANDVKRDER LLRQQLNALL EEERMPMQAR LQRNEAVSSH EAVPQTPLV DLPGVERRRD LPADPITRLF FQHKGDHALY YGTYDKPSVQ DDDRVQIEKR VPRRREENLY TPIYDFCHRI R EATEQRKR ...String:
MLRQSLLSLA KVYGAVPPLG AAQGHRLLHG KREREGSLFA VANDVKRDER LLRQQLNALL EEERMPMQAR LQRNEAVSSH EAVPQTPLV DLPGVERRRD LPADPITRLF FQHKGDHALY YGTYDKPSVQ DDDRVQIEKR VPRRREENLY TPIYDFCHRI R EATEQRKR FVVVPSTIET RGCARVMHDH GLVAGFRDFH NDRAFAVELK YFQGDSTINV IEPCSYDGRT EFEWSPKMMR RL LNTHGIH NRLVVYICRT ADNRIIDHIH AVKENIGGRG LMMVH

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Macromolecule #18: uS9m

MacromoleculeName: uS9m / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 50.201016 KDa
SequenceString: MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE ...String:
MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF ARKVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEKY HFT ALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALV KLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

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Macromolecule #19: uS11m

MacromoleculeName: uS11m / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 37.92591 KDa
SequenceString: MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE ...String:
MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRS E GSTDNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLN GPKMPRGVFK

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Macromolecule #20: uS12m

MacromoleculeName: uS12m / type: protein_or_peptide / ID: 20
Details: mitochondrial gene subject to RNA editing alternative start codon
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 10.421635 KDa
SequenceString:
LFFLRMWFLY GCCLRFVLFV LCYYMSPRLP SSGNRRVLYA VFYLYNFVWM LRCFFCCFIG LVMSLFIIEG GGFVDLPGVK YYTRIVS

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Macromolecule #21: uS15m

MacromoleculeName: uS15m / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 50.401191 KDa
SequenceString: MFVRSFCFLR KYGSKSKTPD LKTSFTKTTN FRKAEKISIT TTDGEIVKGR GKIRRRTHSA LKEAIGAMEH PAIWLWYPWR MNPEPPTPH MPQRRALKNV HGAVFNDLTP VQKKRQEQML YGVNIPETRQ MKFEEQHPLL AGALRKLEGQ PKGFPFWYRK Y PTRRHAYE ...String:
MFVRSFCFLR KYGSKSKTPD LKTSFTKTTN FRKAEKISIT TTDGEIVKGR GKIRRRTHSA LKEAIGAMEH PAIWLWYPWR MNPEPPTPH MPQRRALKNV HGAVFNDLTP VQKKRQEQML YGVNIPETRQ MKFEEQHPLL AGALRKLEGQ PKGFPFWYRK Y PTRRHAYE YRFSIPVEML DGYNDDVKKA LSKGMMSIQE KQFAQEAMYM ERYAEHDFDT TSPAVLAVKR ALKCRVLRNH LL TNPHNNI IKTVLANTER KLNHALRRLR KVDFKKYWEI IRDHDVQDIL QPPNLVTYRQ GSYWKYDWNA GLAISTNLAD VMD PRGLNG CVETGRSRSE VARDLGLSYT RPLHENEKKQ LSHQAVYYER LAKFKMEQPE AARAMERERF VRKFSGMFVK MDIR SGAPD FPSTYRRLLG TKVVRWASKR HGPN

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Macromolecule #22: bS16m

MacromoleculeName: bS16m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 22.077805 KDa
SequenceString:
MQVTETVLAR AVIKRRSPQL WGAPGAPIIR MRGHHVVWKF QSYDLVVEHT HKRRNSDIRL LHYLGKHCPH PQKSLWSPDT PVAQDRHLF MLTTVDIDAF KYWFGVKRCR LSMKPWALLA KAGLLPPSLT QNSKIMPKPL FDKESLMRYY LANRKDEDVM A REKYLNYE NSMVKTEEER AAERPVAPYL

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Macromolecule #23: uS17m

MacromoleculeName: uS17m / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 35.632727 KDa
SequenceString: MLRRSLLAFP WWNFQTEHRQ RCVLMYGGAR TKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLSQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV ATNHMVYYPK FNQRVSRTKR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN ...String:
MLRRSLLAFP WWNFQTEHRQ RCVLMYGGAR TKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLSQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV ATNHMVYYPK FNQRVSRTKR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN VEGRERLKLG LKAVPPPLFG YPVSRRIVKL NLTSTEGTQE KLAAAIQEHV QDAYRFSGPT PDQPRNRLAD PV TFEDANN MIAPNAPAAA ALDASDSPPL LDRGEYTEVE QDTRNKKGDD YWMNLQPKEK YDFKSFKKSP

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Macromolecule #24: bS18m

MacromoleculeName: bS18m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 36.988691 KDa
SequenceString: MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI ...String:
MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKRTVEHF AYNWVQTDRI RRYFRELEAV HTSGSASARG SG GGTTAEH KQQDQFYSPE NQPISLHRNN ISYMEDVGRS VKNPTVPGLM STKGMKKKFH NLYSSTSTKR MGFSNPTLGI KKV

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Macromolecule #25: uS21m

MacromoleculeName: uS21m / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 23.087889 KDa
SequenceString: MLHTTRLWLG GYMMYHRKAM GTMKYSKWKG AHGGISHFYG RTPMVEEVRP NEPITLVDRR IMHYVHHSRL RHFQLFRSYQ EKSNSTECK LREGEMLRRR WHRRLQKSFI AFMQFKTMKV LEDQARLVNT YGQAAVNAAL GDPWNATDNV ARERKSAAVR R QVRALPMV ...String:
MLHTTRLWLG GYMMYHRKAM GTMKYSKWKG AHGGISHFYG RTPMVEEVRP NEPITLVDRR IMHYVHHSRL RHFQLFRSYQ EKSNSTECK LREGEMLRRR WHRRLQKSFI AFMQFKTMKV LEDQARLVNT YGQAAVNAAL GDPWNATDNV ARERKSAAVR R QVRALPMV NVVPKHVATM KQIHNDRFNY RWRVN

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Macromolecule #26: mt-IF-3

MacromoleculeName: mt-IF-3 / type: protein_or_peptide / ID: 26 / Details: residues 294-304 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 41.086285 KDa
SequenceString: MMQRCSTSLS RLCFRRLLRT PLLVYSIPPT RDVPSGIAHC PLSCSMRMVT SSNDDEFVFD PTLSIQKDAA IHTAKKSFET IVLEYVPAH APEEARQKVK SYLTQHPIDI LITQPKVQIT HLEDAESGAE TKVSLSPCDL PEALQQARER GMNLVQMGAR G DVAYCRIR ...String:
MMQRCSTSLS RLCFRRLLRT PLLVYSIPPT RDVPSGIAHC PLSCSMRMVT SSNDDEFVFD PTLSIQKDAA IHTAKKSFET IVLEYVPAH APEEARQKVK SYLTQHPIDI LITQPKVQIT HLEDAESGAE TKVSLSPCDL PEALQQARER GMNLVQMGAR G DVAYCRIR RESTRILSLI HTELEALREQ EEKQQGKGRG GVQAAAKMGE LIDHTFRDAV DAHFVGWRSK KIVEDIRRRH PV KLTIKEF QSPECAIGKL REMCQAMQHY AQEKVIYHHF TSIVANDREA SITFAPALPM AKSDSWKHIK YPGEKEWTNA LRR MEDACR KSGRYGTYAK SNKLKLRSLG QTSYRVDKYG RKMD

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Macromolecule #27: mS22

MacromoleculeName: mS22 / type: protein_or_peptide / ID: 27 / Details: residues 20-37 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 73.064141 KDa
SequenceString: MLRRAYIQRR YPFNKRGPRE HKSWKHHVLT EPPKPLQWRD PKVWTRDLSV MKSFDAPQWD LWQSRPRSED MDEALQPFMD MPKSLKDRR YDIPWWANPF GAWYLQNILS LELLKLKSKT NAEKIATYRS YMRSLASGKD NTMSDDDVIR NIIKERWKTL E FGDRNAGY ...String:
MLRRAYIQRR YPFNKRGPRE HKSWKHHVLT EPPKPLQWRD PKVWTRDLSV MKSFDAPQWD LWQSRPRSED MDEALQPFMD MPKSLKDRR YDIPWWANPF GAWYLQNILS LELLKLKSKT NAEKIATYRS YMRSLASGKD NTMSDDDVIR NIIKERWKTL E FGDRNAGY PCTFGDYIQF LNEWFKSLDE EGMQRLREHF DRRIRPLLAV MSPVDILWLE ALTQNSPHNK EQLQRKIAFQ TS LGTPEFF DMSKRLRYEI NEDYKVRDEL GPELFALWSK APERWPPERL SKMYGLDFTL VRKILVWHHF KACYDACVEP DWS LPKRLF ALEWIRDVRA RKHGLFYGKM RFAEQKITFY SDRFLFRDLV NRREASYANV WEMDDPYRFL QTEQDYEDYW GDNY DVYRR MFPEMIGRTG EPVQQYGQMP IWAGPHRQHA NKSEHNWMFA EIGVNVGHEA LKKLELDPTN EKRRRFVIRQ PDGTL RSAK MSEMRAWYWK EEWADFRFWA PQMEWGIENT PSQEQYQEHV PDTTDADFRK QRRIQSRPVK WFYESHYTRT GSFAGF QPL RFMQRRTERE VRWPDVINAA VQIQKRKPAA YIFKAIPEL

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Macromolecule #28: mS23

MacromoleculeName: mS23 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 36.7845 KDa
SequenceString: MRQCVVRRYK MPKNMGVAPR FDTWNEKYEP WEHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPHNASEPT QDDTSTLSP ERVALRDELA RKSRLVASEG MRYYNIFWVR KPLDTMEKEY YELKRRGVDH GEAIRKVLQG FYSGLAVKKR V AAIQAEEA ...String:
MRQCVVRRYK MPKNMGVAPR FDTWNEKYEP WEHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPHNASEPT QDDTSTLSP ERVALRDELA RKSRLVASEG MRYYNIFWVR KPLDTMEKEY YELKRRGVDH GEAIRKVLQG FYSGLAVKKR V AAIQAEEA KLTGRFITMR EATVVLGVLA KLHKEQLTPH QVSLLAKEQG ETTQSGAKLT AIVSRTQPHV NKEASSPATS EA VGSSTEE SLSADALASM LSEDGEQSAV GTRYQVEVKE TANDSVRQLR EKAEDQTGFP DWYTGESPTY SGTSCSRDGF ALM KANK

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Macromolecule #29: mS26

MacromoleculeName: mS26 / type: protein_or_peptide / ID: 29 / Details: residues 221-248 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 51.136684 KDa
SequenceString: MMRSSSFCRR QIRPYYNLPS KSEHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRRV YKRQWLESFR VNADEYIYKY NITKAAQLA QWECEMKEQE KKRIEARQMM DGRQALKKKH LDLLREFHER QFFFWYERAS ERLQNMNLIN YVPHAQLREH I DKELDKYV ...String:
MMRSSSFCRR QIRPYYNLPS KSEHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRRV YKRQWLESFR VNADEYIYKY NITKAAQLA QWECEMKEQE KKRIEARQMM DGRQALKKKH LDLLREFHER QFFFWYERAS ERLQNMNLIN YVPHAQLREH I DKELDKYV AGKNEPYPLN FVGQMPFLED GDGNIVEVPE SLLSNHMAEH PDSTAKPHEP HTSSSISEAA AFEERMLRAM VS AKEEDLK EWLGDDSRAL SETIDDISRE EEEREADIRV ARSMEETDAE REVSRRAYIE RGKTGSRSIF RPPTVSEGAG GTP SAPAGD ANTPMRRRKK GKLDRVHALQ AHQDELLAKL SSQGLKEGVD ASSVPERGKI VQSRGRIRDK AVIPTHEVLM QKPE LAAGS TPGARIQTKD MVDKMYHRGK YKKSGSGDKS DGEDL

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Macromolecule #30: mS34

MacromoleculeName: mS34 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 28.72818 KDa
SequenceString: MLRCARVALR ADPLNGGSSM TLGSKGSKLS PEPHRRRMPW TAAKEYVPGV VLNARDKMVL DGVQLLDIES IDRASQLDPL EVLRAVVAT REYNISTGKN IFQLASQATY NGRGQRFYRK EWQEGTYDKY VTLSAIDFDR DGNKGTAYGY ITFHGETTTR P VQVDFADV ...String:
MLRCARVALR ADPLNGGSSM TLGSKGSKLS PEPHRRRMPW TAAKEYVPGV VLNARDKMVL DGVQLLDIES IDRASQLDPL EVLRAVVAT REYNISTGKN IFQLASQATY NGRGQRFYRK EWQEGTYDKY VTLSAIDFDR DGNKGTAYGY ITFHGETTTR P VQVDFADV PGWYMDFVEE RAVPFTGIVP PPPSIGTDVP VDPHSYRLKA YPYYDAPNPP EFVERLLKDR GVLPDTPTET AD VDKDPTT SDGSVHYDGK

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Macromolecule #31: mS37

MacromoleculeName: mS37 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 24.757896 KDa
SequenceString: MKSSDIFFAY RLTPVVFKSR QHDSGVNQYG LKPTNAYDYI NPTNLINFGR GTTFDNLGVR RAGRGEIDSS PSHSGSPVFT QAKLIGLSG EEQLTMCQSE TMALRLCMAK AGKETCERES RALDSCLGRV GHLRRAMSEA CWEFNDWFIQ NVSDNHTKPF Q HRPHDWRH ...String:
MKSSDIFFAY RLTPVVFKSR QHDSGVNQYG LKPTNAYDYI NPTNLINFGR GTTFDNLGVR RAGRGEIDSS PSHSGSPVFT QAKLIGLSG EEQLTMCQSE TMALRLCMAK AGKETCERES RALDSCLGRV GHLRRAMSEA CWEFNDWFIQ NVSDNHTKPF Q HRPHDWRH FYAQEKLVRE RQQNGHAYGR RPKQFSFGAR YVKTDGYGKR PRLPYNK

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Macromolecule #32: mS38

MacromoleculeName: mS38 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 28.2406 KDa
SequenceString: MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR ...String:
MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR FQMKKHRIIK RFRFRRYKLA AVANLPFAKM IRVGMLPELK SSKTKRGDVV DPTLSGQLVS AVKNTGEKRK GQ RTRPKSK YQV

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Macromolecule #33: mS41

MacromoleculeName: mS41 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 22.421469 KDa
SequenceString:
MLKRTLTVLD QTYGPHKSYK YTYMPDPRKL APIETTQRSE IVPQSIRPPT SYVPNHETFL ERADIHRLRP TSDFKASFKD WNDLFTCDK RQLRVRGIPR MTRDAIRTAV QAFQNGNPPE RFDTKEEWLY YKQFKTVDYS YRVIPELPEK YRPHQSGIDQ A PLPDYREI NKMPQWAECE ERRQSKKTV

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Macromolecule #34: mS42

MacromoleculeName: mS42 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: superoxide dismutase
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 29.748666 KDa
SequenceString: MRLCRRLSYH AYVPKGASVQ MGETFMPSNI ATAVFRETEA AAARADKTKE ETGFFSQPRL NYPVSSGIPA LFSKDQFDMQ YSLFHRDVV ETLNRHTLGT PLEGHNLETV IRKTSFDATQ AVAHTAASEH FNYCFFYKSL RPWGTAPPPR LREALQLQYG T NGSADAMD ...String:
MRLCRRLSYH AYVPKGASVQ MGETFMPSNI ATAVFRETEA AAARADKTKE ETGFFSQPRL NYPVSSGIPA LFSKDQFDMQ YSLFHRDVV ETLNRHTLGT PLEGHNLETV IRKTSFDATQ AVAHTAASEH FNYCFFYKSL RPWGTAPPPR LREALQLQYG T NGSADAMD EVRRIVWVTA ASHQERCGWV YLVWSGVHFD VVEFPHGSCP IASDLIPLLA LNVHESAYIL DYGTSGLKQY VE NYFKACN WPLAERYYLM AIGQVV

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Macromolecule #35: mS43

MacromoleculeName: mS43 / type: protein_or_peptide / ID: 35 / Details: residues 27-35 built as polyalanines / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 48.59041 KDa
SequenceString: MLRRTVTSLR NARELHRLLP ALLPLLDPPV PTGASVCAAH LIVATGHVSK QREPHMSNCP VLERKIPLSE IASDDNFLKH PCVPCLKHR INFLASEDFC YDFVERCGCG TDGALPPEWR CIVERQFGSM AALVEELVIH ASNRREAGWT WLVYDAQQAG E RPLLVVNM ...String:
MLRRTVTSLR NARELHRLLP ALLPLLDPPV PTGASVCAAH LIVATGHVSK QREPHMSNCP VLERKIPLSE IASDDNFLKH PCVPCLKHR INFLASEDFC YDFVERCGCG TDGALPPEWR CIVERQFGSM AALVEELVIH ASNRREAGWT WLVYDAQQAG E RPLLVVNM PAQRSPLLLG LWPLAVVNVT EAVLVEAVRA ESSGSVAAAP PPPWCRAARA SLYDTKAAAE AFAMTAPMSE GV GKKLSEI REKVARDAVS RMNWGFILQQ LKKAETYYAS SDRGEKIRVH RMKLEREAVT KAMSGLRDSG ATFLTHDTVE VTT TPSANS EPSSPERVPG VQAKQPQTEG MDPCKNKASA EPDPKCTQLP DGTWQYSYRN GDVTLLKPDG TRVFRKRELT TTAY PDGSM LFQYANNTSI LDRADGVRVT TFEDGTTKEE MIRR

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Macromolecule #36: mS47

MacromoleculeName: mS47 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 137.935 KDa
SequenceString: MERRRLLHDG RWFTPCVLYG SARRGLKTLD VREYRPLAMP IEFRFYQRYA NHPNRQSGIQ FLTHYNTHQR FRVNKDYIDY MHWGKEQGQ ARLPHRHQRV AFDFNDQLHP TRIGTPEDGG NANHCYPEDG EDGSYAWFAS QDPTFGHHPD LSSSFDPNYR V FSHPEHWN ...String:
MERRRLLHDG RWFTPCVLYG SARRGLKTLD VREYRPLAMP IEFRFYQRYA NHPNRQSGIQ FLTHYNTHQR FRVNKDYIDY MHWGKEQGQ ARLPHRHQRV AFDFNDQLHP TRIGTPEDGG NANHCYPEDG EDGSYAWFAS QDPTFGHHPD LSSSFDPNYR V FSHPEHWN KMFTKRRPGE GDIDLSVLPS RSLLGPLMAH SDTKGIPYFK VDNRGHSNGR VPGVNAPFFG EFDQKMMQAM SR PLNANRT ITGNDGRFSK TIMINEPQCN QALSAKTASE LSKEIDKATN AVYSKLSVLE AAQSGLTDYF CGGLNFEIVG FEL HMAAML RERAAAILER SGAGGLPSAS SATLSSSSTT RRCAMLLEKA DEREVNKLLR DASRYEDRVD DALRQHASLI WRVY TAPRP LMTLTNGKCR GTGCGLSLFA KYCALKDSSE FIFDGPNVGI TPYGGMTRLL ARPETSLKYP GLAEFVVLTG ASLFA GDAL RLGWTDLFTT IPDMTYHIKE WFDTTEHMHN DAVAWQLGHL LETCFKMKES HSSAMERAAI TPTRARWVED AFADQP SVN DIMRTLTEIE QLPFQSTHNT SDESHITPYT LGSVAAGVQR LGEHRLRYTL SPWDITPPED SVVLKHTSEM FRAYVLE RR GATDVVTHRD AERAAAWSQQ RQREYDAYCS LKEAPHPRHV YARLEGCEGK IVSFEFVFEI SCRRSGPQGT DEVLQTTT I SRLKSRILDA LGMPRDRNID LGWYLPTLDT CPIHSDEELM QVLHTDPGIE DPKEKLRYPP IYFIVKRCTL YFSEWAYAV KHQLLLQSPF ALRAAFGLLQ EVRGDGAAEN VMPLAETLGT EFKYFTRLMR RPDFYRVGVH TDKSVEKWEE IKEERRHNIH HTHRPTRPL PDFEEVFERD VEIDGHRFVL RPRWNPRTLQ DVDDSDILRL RAPLTYDMDS TAALHVPTHC GKASRLAGML Q DAGGVQVL DGLGEVDDKG EPKVPPLQSN ASVPTNVSFY EMARHPWEDR ASSWRRDGFT EGSLEYFESQ YRSAERAVYD EE GRGVRNY WPSRDAVEGI AQEEENDAQL LQERLFNTLE TAGTSVEPWA RNLRLNATTG KFGYKTEIAT QEEKIYDDEY YRW FIQPGR HPNPSGLTLN GRKGQFEEGH SRDEELERMW RKVVADGNND FAATTSGMPV YDEDAKNAED SYAAGDDVDV VTTD ANVPV DGDDSS

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Macromolecule #38: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 38 / Details: unassigned UNK residues / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 2.74137 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #39: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 39 / Details: unassigned UNK residues / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 698.854 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #40: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 4.613678 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #41: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 41 / Details: unassigned UNK residues / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 3.762629 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #37: 9S rRNA

MacromoleculeName: 9S rRNA / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote)
Molecular weightTheoretical: 198.481422 KDa
SequenceString: UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA ...String:
UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG UUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGGUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAUAAUAAAA AUAACGUGCA GUAAUUAAUA UAUUUAUAAA AAUAUAUUUU UUUUUUUU

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Macromolecule #42: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 42 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #43: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 43 / Number of copies: 36 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #44: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 44 / Number of copies: 2 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Macromolecule #45: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 45 / Number of copies: 1 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND
Startup modelType of model: OTHER / Details: initial model from 2D class averages in EMAN
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.27 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 31911
Initial angle assignmentType: OTHER
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION

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Atomic model buiding 1

DetailsWe used Coot and O for initial model building and refined the structure using Phenix
RefinementProtocol: AB INITIO MODEL / Overall B value: 41.4
Output model

PDB-6hiy:
Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the body of the small mitoribosomal subunit in complex with mt-IF-3

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