7REP

Crystal structure of an engineered variant of single-chain Penicillin G Acylase from Kluyvera cryocrescens (A1-Ac Rd3CHis)

Summary for 7REP

• Entry DOI: 10.2210/pdb7rep/pdb
• Related: 7REO
• Descriptor: Penicillin G acylase, CALCIUM ION, phenylmethanesulfonic acid, ... (4 entities in total)
• Functional Keywords: penicillin, acylase, hydrolase
• Biological source: Kluyvera cryocrescens
• Total number of polymer chains: 1
• Total formula weight: 86471.39

Authors

Orth, P. (deposition date: 2021-07-13, release date: 2021-11-17, Last modification date: 2023-10-18)

• Primary citation: Fryszkowska, A.,An, C.,Alvizo, O.,Banerjee, G.,Canada, K.A.,Cao, Y.,DeMong, D.,Devine, P.N.,Duan, D.,Elgart, D.M.,Farasat, I.,Gauthier, D.R.,Guidry, E.N.,Jia, X.,Kong, J.,Kruse, N.,Lexa, K.W.,Makarov, A.A.,Mann, B.F.,Milczek, E.M.,Mitchell, V.,Nazor, J.,Neri, C.,Orr, R.K.,Orth, P.,Phillips, E.M.,Riggins, J.N.,Schafer, W.A.,Silverman, S.M.,Strulson, C.A.,Subramanian, N.,Voladri, R.,Yang, H.,Yang, J.,Yi, X.,Zhang, X.,Zhong, W.; A chemoenzymatic strategy for site-selective functionalization of native peptides and proteins.; Science, 376:1321-1327, 2022; PubMed: 35709255; DOI: 10.1126/science.abn2009

Experimental method

X-RAY DIFFRACTION (2.192 Å)