6ZCC

X-ray structure of the Haloalkane dehalogenase HOB (HaloTag7-based Oligonucleotide Binder) labeled with a chloroalkane-tetramethylrhodamine fluorophore substrate

Summary for 6ZCC

Entry DOI	10.2210/pdb6zcc/pdb
Related	6Y7A 6Y7B
Descriptor	Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, ACETATE ION, ... (5 entities in total)
Functional Keywords	haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, (synthetic) fluorophore, tetramethylrhodamine, oligonucleotide binder, hydrolase
Biological source	Rhodococcus sp.
Total number of polymer chains	1
Total formula weight	34268.95
Authors	Tarnawski, M.,Johnsson, K.,Hiblot, J. (deposition date: 2020-06-10, release date: 2021-04-21, Last modification date: 2024-01-24)
Primary citation	Wilhelm, J.,Kuhn, S.,Tarnawski, M.,Gotthard, G.,Tunnermann, J.,Tanzer, T.,Karpenko, J.,Mertes, N.,Xue, L.,Uhrig, U.,Reinstein, J.,Hiblot, J.,Johnsson, K. Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60:2560-2575, 2021 Cited by PubMed: 34339177 DOI: 10.1021/acs.biochem.1c00258 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.52 Å)

Structure validation