6Y7B

X-ray structure of the Haloalkane dehalogenase HaloTag7 labeled with a chloroalkane-carbopyronine fluorophore substrate

Summary for 6Y7B

• Entry DOI: 10.2210/pdb6y7b/pdb
• Related: 6Y7A
• Descriptor: Haloalkane dehalogenase, 4-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]-2-[3-(dimethylamino)-6-(dimethyl-$l^{4}-azanylidene)-10,10-dimethyl-anthracen-9-yl]benzoic acid, CHLORIDE ION (3 entities in total)
• Functional Keywords: haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, fluorophore, carbopyronine, hydrolase
• Biological source: Rhodococcus sp.
• Total number of polymer chains: 5
• Total formula weight: 169623.50

Authors

Tarnawski, M.,Johnsson, K.,Hiblot, J. (deposition date: 2020-02-28, release date: 2021-03-31, Last modification date: 2021-11-10)

• Primary citation: Wilhelm, J.,Kuhn, S.,Tarnawski, M.,Gotthard, G.,Tunnermann, J.,Tanzer, T.,Karpenko, J.,Mertes, N.,Xue, L.,Uhrig, U.,Reinstein, J.,Hiblot, J.,Johnsson, K.; Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.; Biochemistry, 60:2560-2575, 2021; PubMed: 34339177; DOI: 10.1021/acs.biochem.1c00258

Experimental method

X-RAY DIFFRACTION (3.1 Å)