1ONA

CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE

1ONA の概要

• エントリーDOI: 10.2210/pdb1ona/pdb
• 分子名称: CONCANAVALIN A, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]methyl alpha-D-mannopyranoside, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: plant lectin, manganese, glycoprotein
• 由来する生物種: Canavalia ensiformis (jack bean)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 104943.46

構造登録者

Bouckaert, J.,Maes, D.,Poortmans, F.,Wyns, L.,Loris, R. (登録日: 1996-07-07, 公開日: 1997-09-17, 最終更新日: 2024-05-22)

主引用文献

Loris, R.,Maes, D.,Poortmans, F.,Wyns, L.,Bouckaert, J.
A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes.
J.Biol.Chem., 271:30614-30618, 1996

PubMed Abstract: The structure of concanavalin A in complex with the trimannoside methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside has been determined in a novel space group. In three of the four subunits of the concanavalin A tetramer, the interactions between the protein and the bound saccharide are essentially identical to those reported previously by other authors (Naismith, J. H., and Field, R. A. (1996) J. Biol. Chem. 271, 972-976). In the fourth subunit, however, the alpha1-->3 linkage has a different conformation, resulting in a different part of the alpha1-->3-linked mannose interacting with essentially the same surface of the protein. Furthermore, significant differences are observed in the quaternary associations of the subunits compared with the saccharide-free structures and other carbohydrate complexes, suggesting that the concanavalin A tetramer is a rather flexible entity.

PubMed: 8940035
DOI: 10.1074/jbc.271.48.30614

実験手法

X-RAY DIFFRACTION (2.35 Å)