1KL4

AN ENGINEERED STREPTAVIDIN WITH IMPROVED AFFINITY FOR THE STREP-TAG II PEPTIDE : apo-SAM2

Summary for 1KL4

Entry DOI	10.2210/pdb1kl4/pdb
Related	1KFF 1KL3 1KL5 1rsu
Descriptor	streptavidin (2 entities in total)
Functional Keywords	biotin, protein engineering, strep-tag, streptavidin, peptide binding protein
Biological source	Streptomyces avidinii
Cellular location	Secreted: P22629
Total number of polymer chains	4
Total formula weight	53414.14
Authors	Korndoerfer, I.P.,Skerra, A. (deposition date: 2001-12-11, release date: 2002-04-03, Last modification date: 2023-08-16)
Primary citation	Korndorfer, I.P.,Skerra, A. Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci., 11:883-893, 2002 Cited by PubMed: 11910031 DOI: 10.1110/ps.4150102 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation