1KL5
an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm2-StrepII
Summary for 1KL5
| Entry DOI | 10.2210/pdb1kl5/pdb |
| Related | 1KFF 1KL3 1KL4 1rsu |
| Descriptor | streptavidin, strep-tag II (3 entities in total) |
| Functional Keywords | biotin, protein engineering, strep-tag, streptavidin, peptide binding protein |
| Biological source | Streptomyces avidinii More |
| Cellular location | Secreted: P22629 |
| Total number of polymer chains | 8 |
| Total formula weight | 58111.19 |
| Authors | Korndoerfer, I.P.,Skerra, A. (deposition date: 2001-12-11, release date: 2002-04-03, Last modification date: 2023-08-16) |
| Primary citation | Korndorfer, I.P.,Skerra, A. Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci., 11:883-893, 2002 Cited by PubMed: 11910031DOI: 10.1110/ps.4150102 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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