8U8T

Y229F/V290N Streptomyces coelicolor Laccase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Copper oxidase	polymer	351	38002.1	3	UniProt (Q9XAL8) Pfam (PF07732) Pfam (PF07731) In PDB	Streptomyces coelicolor	Laccase
2	A, C, B	COPPER (II) ION	non-polymer		63.5	12	Chemie (CU)
3	A, C, B	HYDROXIDE ION	non-polymer		17.0	3	Chemie (OH)
4	B, A, C	GLYCINE	non-polymer		75.1	15	Chemie (GLY)
5	B, A, C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
6	B, A, C	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
7	B, A, C	TETRAETHYLENE GLYCOL	non-polymer		194.2	6	Chemie (PG4)
8	A	BORIC ACID	non-polymer		61.8	1	Chemie (BO3)
9		water	water		18.0	141	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 343 (UniProt: Q9XAL8)

PDB	External Database	Details
Phe 229	Tyr 229	engineered mutation
Asn 290	Val 290	engineered mutation
Leu 344	-	expression tag
Glu 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	114006.4
Non-Polymers*	Number of molecules	43
	Total formula weight	3935.6
All*	Total formula weight	117942.0

*Water molecules are not included.