8U8T
Y229F/V290N Streptomyces coelicolor Laccase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 176.987, 176.987, 176.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.150 - 2.900 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.19780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.024 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.150 | 3.004 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.242 | 1.335 |
Number of reflections | 62634 | 6149 |
<I/σ(I)> | 10.23 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.993 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | Crystals were prepared using hanging drop vapor-diffusion technique at room temperature (~296 K). Protein is at a concentration of 18.5 mg/ml in 50 mM H3BO3, 0.1 M NaCl, pH 9.0 buffer. The well buffer contains 0.1 M glycine, 0.3-0.6 M NaCl, pH 9.0, and 37-39% (v/v) PEG (polyethylene glycol) monomethyl ether 550. 500 uL of well buffer is added to each well and protein is mixed with well buffer at a 1.5 uL:1.5 uL ratio. The crystal growth time was ca. 1-2 weeks |