8ODZ

Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Interleukin-12 subunit alpha	polymer	231	25927.5	1	UniProt (P43431) Pfam (PF03039) In PDB	Mus musculus (house mouse)	IL-12A,Cytotoxic lymphocyte maturation factor 35 kDa subunit,CLMF p35,IL-12 subunit p35
2	B	Interleukin-12 subunit beta	polymer	313	35837.3	1	UniProt (P43432) Pfam (PF10420) In PDB	Mus musculus (house mouse)	IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40
3	C	Interleukin-12 receptor subunit beta-1,Death-associated protein kinase 1	polymer	572	63789.2	1	UniProt (Q60837) UniProt (P53355) Pfam (PF00041) UniProt (by SIFTS) (Q80YE7) In PDB	Mus musculus (house mouse)	IL-12 receptor subunit beta-1,IL-12R subunit beta-1,IL-12R-beta-1,IL-12 receptor beta component,DAP kinase 1
4	D	Interleukin-12 receptor subunit beta-2,Calmodulin-1	polymer	769	85723.3	1	UniProt (P97378) UniProt (P0DP23) Pfam (PF06328) Pfam (PF00041) Pfam (PF13499) UniProt (by SIFTS) (P0DP26) In PDB	Mus musculus (house mouse)	IL-12 receptor subunit beta-2,IL-12R subunit beta-2,IL-12R-beta-2,IL-12RB2
5	E	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
6	C, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
7		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 23 - 215 (UniProt: P43431)

PDB	External Database	Details
Gly 216	-	expression tag
Thr 217	-	expression tag
Ser 218	-	expression tag
Asp 219	-	expression tag
Glu 220	-	expression tag
Val 221	-	expression tag
Asp 222	-	expression tag
Gly 223	-	expression tag
Gly 224	-	expression tag
Ser 225	-	expression tag
Gly 226	-	expression tag
Gly 227	-	expression tag
Ser 228	-	expression tag
Gly 229	-	expression tag
Leu 230	-	expression tag
Asn 231	-	expression tag
Asp 232	-	expression tag
Ile 233	-	expression tag
Phe 234	-	expression tag
Glu 235	-	expression tag
Ala 236	-	expression tag
Gln 237	-	expression tag
Lys 238	-	expression tag
Ile 239	-	expression tag
Glu 240	-	expression tag
Trp 241	-	expression tag
His 242	-	expression tag
Glu 243	-	expression tag
Gly 244	-	expression tag
Arg 245	-	expression tag
Thr 246	-	expression tag
Lys 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

C: 20 - 561 (UniProt: Q60837)

PDB	External Database	Details
Gly 562	-	linker
Thr 563	-	linker
Gly 564	-	linker
Gly 565	-	linker
Ser 566	-	linker
Gly 567	-	linker
Gly 568	-	linker
Ser 569	-	linker
Gly 570	-	linker
Gly 571	-	linker

C: 572 - 591 (UniProt: P53355)
D: 24 - 637 (UniProt: P97378)

PDB	External Database	Details
Gly 638	-	linker
Thr 639	-	linker
Gly 640	-	linker
Gly 641	-	linker
Ser 642	-	linker
Gly 643	-	linker
Gly 644	-	linker
Ser 645	-	linker
Gly 646	-	linker
Gly 647	-	linker

D: 648 - 792 (UniProt: P0DP23)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	211277.3
Branched	Number of molecules	1
	Total formula weight	748.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1106.0
All*	Total formula weight	213132.0

*Water molecules are not included.