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8ODZ

Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1).

Functional Information from GO Data
ChainGOidnamespacecontents
A0005125molecular_functioncytokine activity
A0005515molecular_functionprotein binding
A0005576cellular_componentextracellular region
A0005615cellular_componentextracellular space
A0005737cellular_componentcytoplasm
A0005788cellular_componentendoplasmic reticulum lumen
A0005796cellular_componentGolgi lumen
A0007165biological_processsignal transduction
A0008083molecular_functiongrowth factor activity
A0008283biological_processcell population proliferation
A0009986cellular_componentcell surface
A0031904cellular_componentendosome lumen
A0032729biological_processpositive regulation of type II interferon production
A0035711biological_processT-helper 1 cell activation
A0042098biological_processT cell proliferation
A0042102biological_processpositive regulation of T cell proliferation
A0042163molecular_functioninterleukin-12 beta subunit binding
A0042832biological_processdefense response to protozoan
A0043514cellular_componentinterleukin-12 complex
A0045582biological_processpositive regulation of T cell differentiation
A0071222biological_processcellular response to lipopolysaccharide
B0001916biological_processpositive regulation of T cell mediated cytotoxicity
B0002230biological_processpositive regulation of defense response to virus by host
B0002323biological_processnatural killer cell activation involved in immune response
B0002827biological_processpositive regulation of T-helper 1 type immune response
B0002860biological_processpositive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
B0002862biological_processnegative regulation of inflammatory response to antigenic stimulus
B0004896molecular_functioncytokine receptor activity
B0005125molecular_functioncytokine activity
B0005126molecular_functioncytokine receptor binding
B0005515molecular_functionprotein binding
B0005576cellular_componentextracellular region
B0005615cellular_componentextracellular space
B0005737cellular_componentcytoplasm
B0005788cellular_componentendoplasmic reticulum lumen
B0005796cellular_componentGolgi lumen
B0007166biological_processcell surface receptor signaling pathway
B0008083molecular_functiongrowth factor activity
B0008283biological_processcell population proliferation
B0009897cellular_componentexternal side of plasma membrane
B0009986cellular_componentcell surface
B0010033biological_processresponse to organic substance
B0010224biological_processresponse to UV-B
B0016064biological_processimmunoglobulin mediated immune response
B0016477biological_processcell migration
B0019221biological_processcytokine-mediated signaling pathway
B0030101biological_processnatural killer cell activation
B0031904cellular_componentendosome lumen
B0032693biological_processnegative regulation of interleukin-10 production
B0032700biological_processnegative regulation of interleukin-17 production
B0032725biological_processpositive regulation of granulocyte macrophage colony-stimulating factor production
B0032729biological_processpositive regulation of type II interferon production
B0032733biological_processpositive regulation of interleukin-10 production
B0032735biological_processpositive regulation of interleukin-12 production
B0032740biological_processpositive regulation of interleukin-17 production
B0032760biological_processpositive regulation of tumor necrosis factor production
B0032816biological_processpositive regulation of natural killer cell activation
B0032819biological_processpositive regulation of natural killer cell proliferation
B0032946biological_processpositive regulation of mononuclear cell proliferation
B0034393biological_processpositive regulation of smooth muscle cell apoptotic process
B0035722biological_processinterleukin-12-mediated signaling pathway
B0042093biological_processT-helper cell differentiation
B0042098biological_processT cell proliferation
B0042102biological_processpositive regulation of T cell proliferation
B0042104biological_processpositive regulation of activated T cell proliferation
B0042164molecular_functioninterleukin-12 alpha subunit binding
B0042509biological_processregulation of tyrosine phosphorylation of STAT protein
B0042531biological_processpositive regulation of tyrosine phosphorylation of STAT protein
B0042802molecular_functionidentical protein binding
B0042832biological_processdefense response to protozoan
B0043514cellular_componentinterleukin-12 complex
B0044877molecular_functionprotein-containing complex binding
B0045519molecular_functioninterleukin-23 receptor binding
B0045672biological_processpositive regulation of osteoclast differentiation
B0045785biological_processpositive regulation of cell adhesion
B0046982molecular_functionprotein heterodimerization activity
B0048662biological_processnegative regulation of smooth muscle cell proliferation
B0050671biological_processpositive regulation of lymphocyte proliferation
B0050709biological_processnegative regulation of protein secretion
B0050829biological_processdefense response to Gram-negative bacterium
B0051135biological_processpositive regulation of NK T cell activation
B0051142biological_processpositive regulation of NK T cell proliferation
B0051607biological_processdefense response to virus
B0070743cellular_componentinterleukin-23 complex
B0071222biological_processcellular response to lipopolysaccharide
B0071346biological_processcellular response to type II interferon
B1900747biological_processnegative regulation of vascular endothelial growth factor signaling pathway
B1903588biological_processnegative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
D0000086biological_processG2/M transition of mitotic cell cycle
D0000922cellular_componentspindle pole
D0002027biological_processregulation of heart rate
D0005509molecular_functioncalcium ion binding
D0005513biological_processdetection of calcium ion
D0005515molecular_functionprotein binding
D0005576cellular_componentextracellular region
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005819cellular_componentspindle
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005876cellular_componentspindle microtubule
D0005886cellular_componentplasma membrane
D0007186biological_processG protein-coupled receptor signaling pathway
D0008076cellular_componentvoltage-gated potassium channel complex
D0010800biological_processpositive regulation of peptidyl-threonine phosphorylation
D0010801biological_processnegative regulation of peptidyl-threonine phosphorylation
D0010856molecular_functionadenylate cyclase activator activity
D0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
D0016020cellular_componentmembrane
D0016240biological_processautophagosome membrane docking
D0019855molecular_functioncalcium channel inhibitor activity
D0019901molecular_functionprotein kinase binding
D0021762biological_processsubstantia nigra development
D0030017cellular_componentsarcomere
D0031432molecular_functiontitin binding
D0031514cellular_componentmotile cilium
D0031954biological_processpositive regulation of protein autophosphorylation
D0031982cellular_componentvesicle
D0032465biological_processregulation of cytokinesis
D0032516biological_processpositive regulation of phosphoprotein phosphatase activity
D0032991cellular_componentprotein-containing complex
D0034704cellular_componentcalcium channel complex
D0035307biological_processpositive regulation of protein dephosphorylation
D0035458biological_processcellular response to interferon-beta
D0043209cellular_componentmyelin sheath
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044325molecular_functiontransmembrane transporter binding
D0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
D0046872molecular_functionmetal ion binding
D0048306molecular_functioncalcium-dependent protein binding
D0050848biological_processregulation of calcium-mediated signaling
D0051343biological_processpositive regulation of cyclic-nucleotide phosphodiesterase activity
D0051592biological_processresponse to calcium ion
D0055117biological_processregulation of cardiac muscle contraction
D0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
D0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
D0060316biological_processpositive regulation of ryanodine-sensitive calcium-release channel activity
D0071346biological_processcellular response to type II interferon
D0071902biological_processpositive regulation of protein serine/threonine kinase activity
D0072542molecular_functionprotein phosphatase activator activity
D0097225cellular_componentsperm midpiece
D0098901biological_processregulation of cardiac muscle cell action potential
D0140056biological_processorganelle localization by membrane tethering
D1901842biological_processnegative regulation of high voltage-gated calcium channel activity
D1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
D1902494cellular_componentcatalytic complex
D1905913biological_processnegative regulation of calcium ion export across plasma membrane
D1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
Functional Information from PROSITE/UniProt
site_idPS01354
Number of Residues42
DetailsHEMATOPO_REC_L_F3 Long hematopoietin receptor, soluble alpha chains family signature. IkPDPPknLqmkplknsq..VevsWeyPdSWstphsyFsLkFfV
ChainResidueDetails
BILE231-VAL272

site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
DASP664-LEU676
DASP700-PHE712
DASP737-LEU749
DASP773-PHE785

site_idPS01353
Number of Residues58
DetailsHEMATOPO_REC_L_F2 Long hematopoietin receptor, gp130 family signature. NskgaSapthinivdlcgtgllaphqvsaksenmdnil...VtWqp.PkkadsavreYi.VeW
ChainResidueDetails
DASN416-TRP473

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues613
DetailsTOPO_DOM: Extracellular => ECO:0000255
ChainResidueDetails
DASN24-ASN637
CASN73
CASN86
CASN130
CASN144
CASN169
CASN188
CASN330
CASN368
CASN374
CASN401
CASN463
CASN477

site_idSWS_FT_FI2
Number of Residues14
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
DASN48
DASN101
DASN114
DASN142
DASN151
DASN169
DASN179
DASN224
DASN252
DASN279
DASN287
DASN323
DASN391
DASN495

site_idSWS_FT_FI3
Number of Residues20
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0007744|PDB:4HEX, ECO:0007744|PDB:4ZLK
ChainResidueDetails
DASP737
DASP739
DASN741
DTYR743
DGLU748
DASP773
DASP775
DASP777
DGLN779
DGLU784
DASP664
DASP666
DASP668
DTHR670
DGLU675
DASP700
DASP702
DASN704
DTHR706
DGLU711

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:23806337
ChainResidueDetails
DLYS665

site_idSWS_FT_FI5
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29
ChainResidueDetails
DTHR688

site_idSWS_FT_FI6
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
DSER725

site_idSWS_FT_FI7
Number of Residues1
DetailsMOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
DLYS738

site_idSWS_FT_FI8
Number of Residues1
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455, ECO:0007744|PubMed:21183079
ChainResidueDetails
DTYR743

site_idSWS_FT_FI9
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
DSER745

site_idSWS_FT_FI10
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
DTHR754

site_idSWS_FT_FI11
Number of Residues1
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
DLYS759

site_idSWS_FT_FI12
Number of Residues1
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
DTYR782

site_idSWS_FT_FI13
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
DLYS665

219869

PDB entries from 2024-05-15

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