8ITG

Crystal structure of lasso peptide epimerase MslH in complexed with precursor peptide variant MslAW21G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Poly-gamma-glutamate synthesis protein (Capsule biosynthesis protein)	polymer	440	47319.4	1	UniProt (A0A4V2TW40) Pfam (PF09587) In PDB	Streptomyces griseorubiginosus
2	B	Tricyclic peptide MS-271	polymer	42	4439.1	1	UniProt (A0A385ZG42) In PDB	Streptomyces griseorubiginosus
3	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5		water	water		18.0	214	Chemie (HOH)

Sequence modifications

A: 1 - 440 (UniProt: A0A4V2TW40)

PDB	External Database	Details
Arg 34	His 34	conflict
Val 59	Ala 59	conflict
Met 101	Leu 101	conflict
His 287	Asp 287	conflict
Ala 380	Val 380	conflict
Ile 425	Val 425	conflict

B: -20 - 20 (UniProt: A0A385ZG42)

PDB	External Database	Details
Gly 21	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51758.5
Non-Polymers*	Number of molecules	2
	Total formula weight	162.2
All*	Total formula weight	51920.7

*Water molecules are not included.