8DO1
Cryo-EM structure of the human Sec61 complex inhibited by ipomoeassin F
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Protein transport protein Sec61 subunit alpha isoform 1 | polymer | 476 | 52202.4 | 1 | UniProt (P61619) Pfam (PF10559) Pfam (PF00344) In PDB | Homo sapiens (human) | Sec61 alpha-1 |
2 | B | Protein transport protein Sec61 subunit gamma | polymer | 68 | 7752.3 | 1 | UniProt (P60059) Pfam (PF00584) In PDB | Homo sapiens (human) | |
3 | C | Protein transport protein Sec61 subunit beta | polymer | 96 | 9987.5 | 1 | UniProt (P60468) Pfam (PF03911) In PDB | Homo sapiens (human) | |
4 | A | [(1~{S},3~{R},4~{S},5~{R},6~{R},8~{R},10~{S},23~{R},24~{R},25~{R},26~{R})-5-acetyloxy-6-methyl-4,26-bis(oxidanyl)-17,20-bis(oxidanylidene)-10-pentyl-24-[(~{E})-3-phenylprop-2-enoyl]oxy-2,7,9,21,27-pentaoxatricyclo[21.3.1.0^{3,8}]heptacosan-25-yl] (~{E})-2-methylbut-2-enoate | non-polymer | 831.0 | 1 | Chemie (SXF) | |||
5 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 476 (UniProt: P61619)
PDB | External Database | Details |
---|---|---|
Tyr 263 | Val 263 | conflict |
Glu 264 | Asp 264 | engineered mutation |
Arg 268 | Lys 268 | engineered mutation |
Thr 270 | Ala 270 | engineered mutation |
Lys 271 | Arg 271 | engineered mutation |
Val 272 | Tyr 272 | engineered mutation |
Ile 276 | Tyr 276 | engineered mutation |
Gly 277 | Asn 277 | engineered mutation |
Ile 278 | Thr 278 | engineered mutation |
Pro 387 | Ala 387 | conflict |
Arg 388 | Lys 388 | conflict |
Ile 390 | Val 390 | conflict |
Phe 394 | Leu 394 | engineered mutation |
Asp 396 | Glu 396 | conflict |
Gly 398 | Gln 398 | conflict |
Ile 401 | Met 401 | engineered mutation |
Asn 402 | Arg 402 | engineered mutation |
Lys 404 | His 404 | engineered mutation |
Ile 409 | Met 409 | engineered mutation |
Tyr 410 | Val 410 | engineered mutation |
Arg 411 | His 411 | engineered mutation |
Lys 414 | Asn 414 | conflict |
Lys 415 | Arg 415 | conflict |
Ile 416 | Tyr 416 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 69942.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 831.0 | |
All* | Total formula weight | 70773.2 |