8D4E

Asymmetric unit of AP-1, Arf1, Nef lattice on MHC-I lipopeptide incorporated wide(r) membrane tubes

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, H, Z	ADP-ribosylation factor 1	polymer	180	20590.5	3	UniProt (P84077) Pfam (PF00025) In PDB	Homo sapiens (human)
2	N, L	Protein Nef	polymer	212	24154.0	2	UniProt (Q90VU7) Pfam (PF00469) In PDB	Human immunodeficiency virus 1	3'ORF,Negative factor,F-protein
3	Y	HLA class I histocompatibility antigen, A alpha chain	polymer	39	4139.4	1	UniProt (P04439) Pfam (PF06623) In PDB	Homo sapiens (human)	Human leukocyte antigen A,HLA-A
4	B	AP-1 complex subunit beta-1	polymer	949	104736.5	1	UniProt (Q10567) Pfam (PF01602) Pfam (PF02883) Pfam (PF09066) In PDB	Homo sapiens (human)	Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit
5	G	AP-1 complex subunit gamma-1	polymer	601	68194.1	1	UniProt (P22892) Pfam (PF01602) In PDB	Mus musculus (house mouse)	Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1
6	M	AP-1 complex subunit mu-1	polymer	422	48475.5	1	UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB	Mus musculus (house mouse)	AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin
7	S	AP-1 complex subunit sigma-3	polymer	154	18305.3	1	UniProt (Q96PC3) Pfam (PF01217) In PDB	Homo sapiens (human)	Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin
8	C, H, Z	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP)
9	C, H, Z	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

N, L: 2 - 206 (UniProt: Q90VU7)

PDB	External Database	Details
Gly 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag

Y: 334 - 365 (UniProt: P04439)

PDB	External Database	Details
Cys 333	-	expression tag
Ser 345	Thr 345	engineered mutation
Gly 349	Ser 349	engineered mutation
Ser 355	Gly 355	engineered mutation
Ala 363	Cys 363	engineered mutation
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag

B: 1 - 949 (UniProt: Q10567)

PDB	External Database	Details
Arg 359	Lys 359	engineered mutation
Lys 476	Glu 476	engineered mutation

G: 1 - 595 (UniProt: P22892)

PDB	External Database	Details
Glu 596	-	expression tag
Asn 597	-	expression tag
Leu 598	-	expression tag
Tyr 599	-	expression tag
Phe 600	-	expression tag
Gln 601	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	353930.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1642.5
All*	Total formula weight	355573.0

*Water molecules are not included.