8C8J

Long Interspersed Nuclear Element 1 (LINE-1) reverse transcriptase ternary complex with hybrid duplex and dTTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RNA-directed DNA polymerase	polymer	832	98020.7	1	UniProt (G8I2S9) Pfam (PF00078) UniProt (by SIFTS) (O00370) In PDB	Homo sapiens (human)
2	B	DNA (5'-D(P*GP*CP*GP*CP*TP*TP*TP*CP*C)-3')	polymer	9	2682.8	1		synthetic construct
3	C	RNA (5'-R(P*UP*UP*AP*GP*GP*AP*AP*AP*GP*CP*GP*C)-3')	polymer	12	3875.4	1		synthetic construct
4	A	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
5	A	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	1	Chemie (TTP)
6	A, C	1,2-ETHANEDIOL	non-polymer		62.1	14	Chemie (EDO)
7	A	DIMETHYL SULFOXIDE	non-polymer		78.1	2	Chemie (DMS)
8	A	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	1	Chemie (DIO)
9	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
10	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
11		water	water		18.0	219	Chemie (HOH)

Sequence modifications

A: 238 - 1061 (UniProt: G8I2S9)

PDB	External Database	Details
Asp 434	Asn 434	conflict
His 910	Leu 910	conflict
His 1062	-	expression tag
His 1063	-	expression tag
His 1064	-	expression tag
His 1065	-	expression tag
His 1066	-	expression tag
His 1067	-	expression tag
His 1068	-	expression tag
His 1069	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	104578.8
Non-Polymers*	Number of molecules	23
	Total formula weight	2063.8
All*	Total formula weight	106642.6

*Water molecules are not included.