8BSU
Crystal structure of the kainate receptor GluK3-H523A ligand binding domain in complex with kainate and the positive allosteric modulator BPAM344 at 2.9A resolution
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | Glutamate receptor ionotropic, kainate 3 | polymer | 258 | 29025.4 | 8 | UniProt (P42264) Pfam (PF10613) Pfam (PF00060) In PDB | Rattus norvegicus (Norway rat) | GluK3,Glutamate receptor 7,GluR-7,GluR7 |
2 | C, A, F, D, B... | 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide | non-polymer | 242.3 | 8 | Chemie (2J9) | |||
3 | E, H, C, A, F... | ZINC ION | non-polymer | 65.4 | 20 | Chemie (ZN) | |||
4 | E, H, C, A, D... | GLYCEROL | non-polymer | 92.1 | 13 | Chemie (GOL) | |||
5 | B, G, H, C, E... | CHLORIDE ION | non-polymer | 35.5 | 22 | Chemie (CL) | |||
6 | B, G, H, E, A... | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
7 | B, G, H, E, C... | 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE | non-polymer | 213.2 | 8 | Chemie (KAI) | |||
8 | B, A, F | ACETATE ION | non-polymer | 59.0 | 3 | Chemie (ACT) | |||
9 | water | water | 18.0 | 115 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 4 - 118 (UniProt: P42264)
A, B, C, D, E, F, G, H: 121 - 258 (UniProt: P42264)
PDB | External Database | Details |
---|---|---|
Gly 1 | - | expression tag |
Pro 2 | - | expression tag |
Gly 3 | - | expression tag |
Ala 95 | His 523 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 119 | - | linker |
Thr 120 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 232203.1 | |
Non-Polymers* | Number of molecules | 82 |
Total formula weight | 7875.0 | |
All* | Total formula weight | 240078.1 |