8A40
Structure of mammalian Pol II-TFIIS elongation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA-directed RNA polymerase subunit | polymer | 1984 | 218889.5 | 1 | UniProt (A0A7M4DUC2) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB | Sus scrofa (pig) | |
| 2 | B | DNA-directed RNA polymerase subunit beta | polymer | 1251 | 142426.1 | 1 | UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB | Sus scrofa (pig) | |
| 3 | C | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) In PDB | Sus scrofa (pig) | |
| 4 | D | RNA polymerase II subunit D | polymer | 142 | 16331.3 | 1 | UniProt (A0A287ADR4) Pfam (PF03874) UniProt (by SIFTS) (A0A4X1VM56) In PDB | Sus scrofa (pig) | |
| 5 | E | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24514.2 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) In PDB | Sus scrofa (pig) | RPB5 homolog |
| 6 | F | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14491.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 7 | G | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) In PDB | Sus scrofa (pig) | |
| 8 | H | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) In PDB | Sus scrofa (pig) | |
| 9 | I | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) In PDB | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) Pfam (PF01194) UniProt (by SIFTS) (A0A8W4F9W9) In PDB | Sus scrofa (pig) | |
| 11 | K | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) Pfam (PF01193) In PDB | Sus scrofa (pig) | |
| 12 | L | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A8D0JYF1) Pfam (PF03604) UniProt (by SIFTS) (A0A4X1TRS6) In PDB | Sus scrofa (pig) | |
| 13 | N | Non-template DNA | polymer | 48 | 14932.5 | 1 | synthetic construct | ||
| 14 | P | RNA | polymer | 14 | 4508.8 | 1 | synthetic construct | ||
| 15 | T | Template DNA | polymer | 41 | 12464.9 | 1 | synthetic construct | ||
| 16 | U | Transcription elongation factor A protein 1 | polymer | 304 | 34295.0 | 1 | UniProt (P23193) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096) In PDB | Homo sapiens (human) | Transcription elongation factor S-II protein 1,Transcription elongation factor TFIIS.o |
| 17 | I, U, A, B | ZINC ION | non-polymer | 65.4 | 11 | Chemie (ZN) | |||
| 18 | A | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
E: 1 - 210 (UniProt: A0A4X1VTX4)
F: 1 - 127 (UniProt: A0A4X1VEK9)
U: 1 - 301 (UniProt: P23193)
| PDB | External Database | Details |
|---|---|---|
| Gly 32 | Glu 32 | conflict |
| Gly 46 | Asp 46 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Thr 126 | Ser 126 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 593293.9 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 792.4 | |
| All* | Total formula weight | 594086.3 |
