7XXH

Cryo-EM structure of the purinergic receptor P2Y1R in complex with 2MeSADP and G11

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	P2Y purinoceptor 1	polymer	413	46296.9	1	UniProt (P47900) Pfam (PF00001) In PDB	Homo sapiens (human)	P2Y1,ADP receptor,Purinergic receptor
2	A	Guanine nucleotide-binding protein G(11) subunit alpha	polymer	353	41385.3	1	Pfam (PF00503) UniProt (by SIFTS) (P63096) UniProt (by SIFTS) (P29992) In PDB	Homo sapiens
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38245.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	H	scfv16	polymer	247	26337.3	1		Homo sapiens
6	R	2-(methylsulfanyl)adenosine 5'-(trihydrogen diphosphate)	non-polymer		473.3	1	Chemie (6AD)

Sequence modifications

R: 2 - 373 (UniProt: P47900)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Glu 374	-	expression tag
Phe 375	-	expression tag
Leu 376	-	expression tag
Glu 377	-	expression tag
Val 378	-	expression tag
Leu 379	-	expression tag
Phe 380	-	expression tag
Gln 381	-	expression tag
Gly 382	-	expression tag
Pro 383	-	expression tag
Gly 384	-	expression tag
Ser 385	-	expression tag
Trp 386	-	expression tag
Ser 387	-	expression tag
His 388	-	expression tag
Pro 389	-	expression tag
Gln 390	-	expression tag
Phe 391	-	expression tag
Glu 392	-	expression tag
Lys 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
Gly 396	-	expression tag
Ala 397	-	expression tag
Gly 398	-	expression tag
Ala 399	-	expression tag
Ser 400	-	expression tag
Ala 401	-	expression tag
Gly 402	-	expression tag
Ser 403	-	expression tag
Trp 404	-	expression tag
Ser 405	-	expression tag
His 406	-	expression tag
Pro 407	-	expression tag
Gln 408	-	expression tag
Phe 409	-	expression tag
Glu 410	-	expression tag
Lys 411	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160126.4
Non-Polymers*	Number of molecules	1
	Total formula weight	473.3
All*	Total formula weight	160599.7

*Water molecules are not included.