7VZQ
The structure of GdmN V24Y/G157A/R158A/G188R mutant in complex with carbamoyl adenylate intermediate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | GdmN | polymer | 702 | 76629.1 | 2 | UniProt (Q84G19) Pfam (PF02543) Pfam (PF16861) In PDB | Streptomyces hygroscopicus | |
2 | A | PHOSPHORIC ACID MONO(FORMAMIDE)ESTER | non-polymer | 141.0 | 1 | Chemie (CP) | |||
3 | A, B | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
4 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
5 | A, B | 5'-O-[(S)-(carbamoyloxy)(hydroxy)phosphoryl]adenosine | non-polymer | 390.2 | 2 | Chemie (CA0) | |||
6 | A, B | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
7 | B | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
8 | water | water | 18.0 | 261 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 682 (UniProt: Q84G19)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Tyr 24 | Val 24 | engineered mutation |
Ala 157 | Gly 157 | engineered mutation |
Ala 158 | Arg 158 | engineered mutation |
Arg 188 | Gly 188 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 153258.2 | |
Non-Polymers* | Number of molecules | 20 |
Total formula weight | 2209.6 | |
All* | Total formula weight | 155467.7 |