7SJ9

13pf E254A microtubule from recombinant human tubulin decorated with EB3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, J, K...	Tubulin alpha-1B chain	polymer	457	50975.3	6	UniProt (P68363) Pfam (PF00091) Pfam (PF03953) In PDB	Homo sapiens (Human)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D, F, G, H...	Tubulin beta-3 chain	polymer	456	51276.4	6	UniProt (Q13509) Pfam (PF00091) Pfam (PF03953) In PDB	Homo sapiens (Human)	Tubulin beta-4 chain,Tubulin beta-III
3	M, N	Microtubule-associated protein RP/EB family member 3	polymer	281	32021.2	2	UniProt (Q9UPY8) Pfam (PF00307) Pfam (PF03271) In PDB	Homo sapiens (Human)	EB1 protein family member 3,EBF3,End-binding protein 3,EB3,RP3
4	G, H, I, J, K...	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	12	Chemie (GTP)
5	G, H, I, J, K...	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)

Sequence modifications

A, C, E, J, K, L: 1 - 451 (UniProt: P68363)

PDB	External Database	Details
His 37	-	insertion
His 37	-	insertion
His 37	-	insertion
His 40	-	insertion
His 41	-	insertion
His 42	-	insertion
Ala 254	Glu 254	engineered mutation

B, D, F, G, H, I: 1 - 450 (UniProt: Q13509)

PDB	External Database	Details
Glu 451	-	expression tag
Asn 452	-	expression tag
Leu 453	-	expression tag
Tyr 454	-	expression tag
Phe 455	-	expression tag
Gln 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	677552.3
Non-Polymers*	Number of molecules	24
	Total formula weight	6569.8
All*	Total formula weight	684122.1

*Water molecules are not included.