7RPX

Archaeal DNA ligase and heterotrimeric PCNA in complex with end-joined DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase sliding clamp 1	polymer	258	28712.0	1	UniProt (P57766) Pfam (PF00705) In PDB	Saccharolobus solfataricus (Sulfolobus solfataricus)	Proliferating cell nuclear antigen homolog 1,PCNA1
2	B	DNA polymerase sliding clamp 2	polymer	245	27461.1	1	UniProt (Q97Z84) Pfam (PF00705) Pfam (PF02747) In PDB	Saccharolobus solfataricus (Sulfolobus solfataricus)	Proliferating cell nuclear antigen homolog 2,PCNA2
3	C	DNA polymerase sliding clamp 3	polymer	252	28560.3	1	UniProt (P57765) Pfam (PF00705) Pfam (PF02747) In PDB	Saccharolobus solfataricus (Sulfolobus solfataricus)	Proliferating cell nuclear antigen homolog 3,PCNA3
4	X	End-joined DNA	polymer	47	14549.3	1		Homo sapiens
5	Z	Template strand DNA	polymer	47	14403.3	1		Homo sapiens
6	E	DNA ligase	polymer	621	69998.4	1	UniProt (Q980T8) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) In PDB	Saccharolobus solfataricus (Sulfolobus solfataricus)	Polydeoxyribonucleotide synthase [ATP]
7	X, E	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

A: 2 - 249 (UniProt: P57766)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Leu 250	-	expression tag
Glu 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag

C: 1 - 244 (UniProt: P57765)

PDB	External Database	Details
Leu 245	-	expression tag
Glu 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag

E: 1 - 601 (UniProt: Q980T8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	183684.3
Non-Polymers*	Number of molecules	3
	Total formula weight	164.8
All*	Total formula weight	183849.1

*Water molecules are not included.