7R78

cryo-EM structure of DNMT5 quaternary complex with hemimethylated DNA, AMP-PNP and SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA repair protein Rad8	polymer	2348	263811.7	1	UniProt (J9VI03) Pfam (PF00385) Pfam (PF00145) Pfam (PF00176) Pfam (PF00271) In PDB	Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
2	D	DNA (5'-D(P*CP*AP*GP*(5CM)P*GP*CP*AP*T)-3')	polymer	36	11168.2	1		Cryptococcus neoformans
3	E	DNA (5'-D(*TP*GP*CP*GP*CP*TP*GP*AP*CP*A)-3')	polymer	36	10999.1	1		Cryptococcus neoformans
4	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
5	A	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
6	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
7	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 58 - 2377 (UniProt: J9VI03)

PDB	External Database	Details
Met 30	-	expression tag
Ser 31	-	expression tag
Tyr 32	-	expression tag
Tyr 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
Asp 40	-	expression tag
Tyr 41	-	expression tag
Asp 42	-	expression tag
Ile 43	-	expression tag
Pro 44	-	expression tag
Thr 45	-	expression tag
Thr 46	-	expression tag
Glu 47	-	expression tag
Asn 48	-	expression tag
Leu 49	-	expression tag
Tyr 50	-	expression tag
Phe 51	-	expression tag
Gln 52	-	expression tag
Gly 53	-	expression tag
Ala 54	-	expression tag
Met 55	-	expression tag
Gly 56	-	expression tag
Ser 57	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	285978.9
Non-Polymers*	Number of molecules	8
	Total formula weight	1242.0
All*	Total formula weight	287220.9

*Water molecules are not included.