7R78
cryo-EM structure of DNMT5 quaternary complex with hemimethylated DNA, AMP-PNP and SAH
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | PHENIX,SerialEM,Coot,RELION |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |