7PQQ

Structure of thermostabilised human NTCP in complex with Megabody 91

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sodium/bile acid cotransporter	polymer	333	36652.4	1	UniProt (Q14973) Pfam (PF01758) In PDB	Homo sapiens (human)	Cell growth-inhibiting gene 29 protein,Na(+)/bile acid cotransporter,Na(+)/taurocholate transport protein,Sodium/taurocholate cotransporting polypeptide,Solute carrier family 10 member 1, NTCP
2	B	Anti-RON nanobody,Megabody 91,Glucosidase YgjK	polymer	906	101583.0	1	UniProt (P42592) Pfam (PF21152) In PDB	Lama glama (llama)	Megabody 91

Sequence modifications

A: 3 - 328 (UniProt: Q14973)

PDB	External Database	Details
Met 2	-	initiating methionine
Thr 5	Asn 5	engineered mutation
Thr 11	Asn 11	engineered mutation
Val 33	Phe 33	engineered mutation
Ile 37	Phe 37	engineered mutation
Asn 86	Lys 86	engineered mutation
Ile 95	Val 95	engineered mutation
Ile 107	Val 107	engineered mutation
Leu 129	Cys 129	engineered mutation
His 221	Leu 221	engineered mutation
Leu 329	-	expression tag
Glu 330	-	expression tag
Val 331	-	expression tag
Leu 332	-	expression tag
Phe 333	-	expression tag
Gln 334	-	expression tag

B: 1 - 12 (PDB: 7PQQ)
B: 13 - 187 (UniProt: P42592)
B: 188 - 906 (PDB: 7PQQ)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	138235.4
All*	Total formula weight	138235.4

*Water molecules are not included.