7PI6

Trypanosoma brucei ISG65 bound to human complement C3d

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	65 kDa invariant surface glycoprotein	polymer	400	44369.9	2	UniProt (Q587F5) Pfam (PF11727) In PDB	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
2	B, D	Complement C3dg fragment	polymer	293	32711.4	2	UniProt (P01024) Pfam (PF07678) In PDB	Homo sapiens (Human)
3	A, B, C, D	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	D	2-(2-METHOXYETHOXY)ETHANOL	non-polymer		120.1	1	Chemie (PG0)
5		water	water		18.0	60	Chemie (HOH)

Sequence modifications

A, C: 24 - 385 (UniProt: Q587F5)

PDB	External Database	Details
Ala 22	-	expression tag
Ala 23	-	expression tag
Asn 33	Lys 33	conflict
Lys 55	Glu 55	conflict
Asp 111	Asn 111	conflict
Asp 130	Glu 130	conflict
Glu 140	Lys 140	conflict
Asp 152	Glu 152	conflict
His 153	Tyr 153	conflict
Lys 154	Arg 154	conflict
Ser 174	Arg 174	conflict
Asn 188	Lys 188	conflict
Glu 238	Met 238	conflict
His 260	Pro 260	conflict
Lys 266	Glu 266	conflict
Lys 269	Arg 269	conflict
Gly 273	Asp 273	conflict
His 275	Tyr 275	conflict
Glu 276	Gln 276	conflict
Lys 277	Arg 277	conflict
Arg 279	Lys 279	conflict
Arg 280	Lys 280	conflict
Ala 306	Thr 306	conflict
Glu 314	Asp 314	conflict
Val 330	Ala 330	conflict
Pro 343	Ser 343	conflict
Gly 368	Ser 368	conflict
Glu 371	Asp 371	conflict
Ala 373	Thr 373	conflict
Lys 384	Arg 384	conflict
Gly 386	-	expression tag
Ser 387	-	expression tag
Gly 388	-	expression tag
Ser 389	-	expression tag
Gly 390	-	expression tag
Ser 391	-	expression tag
Ala 392	-	expression tag
Ser 393	-	expression tag
Gly 394	-	expression tag
Gly 395	-	expression tag
Leu 396	-	expression tag
Asn 397	-	expression tag
Asp 398	-	expression tag
Ile 399	-	expression tag
Phe 400	-	expression tag
Glu 401	-	expression tag
Ala 402	-	expression tag
Gln 403	-	expression tag
Lys 404	-	expression tag
Ile 405	-	expression tag
Glu 406	-	expression tag
Trp 407	-	expression tag
His 408	-	expression tag
Glu 409	-	expression tag
Gly 410	-	expression tag
Gly 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag

B, D: 996 - 1287 (UniProt: P01024)

PDB	External Database	Details
Gly 995	-	expression tag
Ala 1010	Cys 1010	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154162.7
Non-Polymers*	Number of molecules	6
	Total formula weight	580.6
All*	Total formula weight	154743.4

*Water molecules are not included.