7OLM
Dioxygenase AsqJ mutant (V72I) in complex with 2b-O-O and Tris
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Iron/alpha-ketoglutarate-dependent dioxygenase asqJ | polymer | 308 | 33992.9 | 1 | UniProt (Q5AR53) Pfam (PF05721) In PDB | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) | 4'-methoxyviridicatin/aspoquinolone biosynthesis cluster protein asqJ,Aspoquinolone biosynthesis protein J |
| 2 | A | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
| 3 | A | 3-[(S)-dioxidanyl(phenyl)methyl]-4-methyl-2-oxidanyl-1H-1,4-benzodiazepin-5-one | non-polymer | 312.3 | 1 | Chemie (VJQ) | |||
| 4 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | A | BROMIDE ION | non-polymer | 79.9 | 1 | Chemie (BR) | |||
| 6 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | water | water | 18.0 | 195 | Chemie (HOH) |
Sequence modifications
A: 2 - 308 (UniProt: Q5AR53)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Ile 72 | Val 180 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33992.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 608.5 | |
| All* | Total formula weight | 34601.4 |
