7MFP

crystal structure of the L136 aminotransferase K185A from acanthamoeba polyphaga mimivirus in the presence of the UDP-viosamine external aldimine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L136 aminotransferase	polymer	356	40230.7	4	UniProt (Q5UPL1) Pfam (PF01041) In PDB	Acanthamoeba polyphaga mimivirus (APMV)
2	A, B, C, D	(2R,3R,4S,5S,6R)-3,4-dihydroxy-5-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-6-methyloxan-2-yl [(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxyoxolan-2-yl]methyl dihydrogen diphosphate (non-preferred name)	non-polymer		778.4	4	Chemie (Z7P)
3	A, B, C, D	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
4	A, B, C, D	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
5	B, C	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6		water	water		18.0	1354	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 352 (UniProt: Q5UPL1)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 185	Lys 185	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	160922.6
Non-Polymers*	Number of molecules	20
	Total formula weight	3815.8
All*	Total formula weight	164738.4

*Water molecules are not included.