7JZV
Cryo-EM structure of the BRCA1-UbcH5c/BARD1 E3-E2 module bound to a nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | BRCA1,Ubiquitin-conjugating enzyme E2 D3 | polymer | 258 | 28974.5 | 1 | UniProt (A0A386IN42) UniProt (P61077) Pfam (PF00097) Pfam (PF00179) UniProt (by SIFTS) (P38398) In PDB | Homo sapiens (Human) | BRCA1-UbcH5c,(E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-conjugating enzyme E2(17)KB 3,Ubiquitin-conjugating enzyme E2-17 kDa 3,Ubiquitin-protein ligase D3 |
| 2 | B | BRCA1-associated RING domain protein 1 | polymer | 115 | 12983.0 | 1 | UniProt (Q99728) Pfam (PF14835) In PDB | Homo sapiens (Human) | BARD-1,RING-type E3 ubiquitin transferase BARD1 |
| 3 | N, n | Histone H2A type 2-A | polymer | 133 | 14310.7 | 2 | UniProt (Q6FI13) Pfam (PF00125) Pfam (PF16211) In PDB | Homo sapiens (Human) | H2A-clustered histone 18,H2A-clustered histone 19,Histone H2A.2,Histone H2A/o |
| 4 | O, o | Histone H2B type 1-K | polymer | 125 | 13790.0 | 2 | UniProt (O60814) Pfam (PF00125) In PDB | Homo sapiens (Human) | H2B K,HIRA-interacting protein 1 |
| 5 | P, p | Histone H3.2 | polymer | 135 | 15257.8 | 2 | UniProt (Q71DI3) Pfam (PF00125) In PDB | Homo sapiens (Human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 6 | Q, q | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) Pfam (PF15511) In PDB | Homo sapiens (Human) | |
| 7 | X | Widom 601 153-bp | polymer | 153 | 47457.2 | 1 | synthetic construct | ||
| 8 | Y | Widom 601 153-bp | polymer | 153 | 46998.9 | 1 | synthetic construct | ||
| 9 | A, B | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A: 2 - 104 (UniProt: A0A386IN42)
A: 202 - 347 (UniProt: P61077)
N, n: 1 - 129 (UniProt: Q6FI13)
P, p: 1 - 135 (UniProt: Q71DI3)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Gly 105 | - | linker |
| Ser 106 | - | linker |
| Gly 107 | - | linker |
| Ser 108 | - | linker |
| Gly 109 | - | linker |
| Ser 110 | - | linker |
| Gly 111 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 285 | Cys 85 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 110 | Cys 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 245657.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 245918.9 |
