7B4J

Thermostable omega transaminase PjTA-R6 variant W58M/F86L/R417L engineered for asymmetric synthesis of enantiopure bulky amines

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Aspartate aminotransferase family protein	polymer	464	50624.8	2	UniProt (A0A2D8IND4) Pfam (PF00202) UniProt (by SIFTS) (A0A2W0F5X5) In PDB	Pseudomonas sp.
2	A, B	4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE	non-polymer		248.2	2	Chemie (PMP)
3	B	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
4		water	water		18.0	382	Chemie (HOH)

Sequence modifications

A, B: 1 - 455 (UniProt: A0A2D8IND4)

PDB	External Database	Details
Ala 9	Pro 9	engineered mutation
Gln 38	Glu 38	engineered mutation
Met 58	Trp 58	engineered mutation
Val 60	Ala 60	engineered mutation
Leu 86	Phe 86	engineered mutation
Asn 87	Ser 87	engineered mutation
Phe 128	Met 128	engineered mutation
Val 154	Ile 154	engineered mutation
Leu 417	Arg 417	engineered mutation
Pro 456	-	expression tag
Gly 457	-	expression tag
Gly 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	101249.5
Non-Polymers*	Number of molecules	3
	Total formula weight	614.4
All*	Total formula weight	101864.0

*Water molecules are not included.