7AKO

Crystal structure of CHK1 kinase domain in complex with a CLASPIN phosphopeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Serine/threonine-protein kinase Chk1	polymer	300	34357.4	2	UniProt (O14757) Pfam (PF00069) In PDB	Homo sapiens (Human)	CHK1 checkpoint homolog,Cell cycle checkpoint kinase,Checkpoint kinase-1
2	C, D	Claspin	polymer	16	1896.0	2	UniProt (Q9HAW4) In PDB	Homo sapiens	hClaspin
3	A, B	STAUROSPORINE	non-polymer		466.5	2	Chemie (STU)
4	A, B	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
5	C	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6		water	water		18.0	314	Chemie (HOH)

Sequence modifications

A, B: 2 - 289 (UniProt: O14757)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Arg 10	Asp 10	engineered mutation
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72506.7
Non-Polymers*	Number of molecules	10
	Total formula weight	1432.9
All*	Total formula weight	73939.7

*Water molecules are not included.